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The map label for this gene is tuf [H]

Identifier: 161598436

GI number: 161598436

Start: 5373828

End: 5375018

Strand: Reverse

Name: tuf [H]

Synonym: nfa50750

Alternate gene names: 161598436

Gene position: 5375018-5373828 (Counterclockwise)

Preceding gene: 54027050

Following gene: 54027048

Centisome position: 89.27

GC content: 65.83

Gene sequence:

>1191_bases
GTGGCGAAGGCGAAGTTCGAGCGGACGAAGCCGCACGTCAACATCGGCACCATCGGTCACGTCGACCACGGCAAGACCAC
GCTGACCGCGGCGATCACCAAGGTGCTGGCTGACAAGTACCCGGATCTGAACCAGAGCTTCGCGTTCGATCAGATCGACA
AGGCGCCCGAAGAGAAGGCGCGTGGCATCACGATCAACATCTCGCACGTCGAGTACCAGACCGAGAAGCGCCACTACGCC
CACGTCGACGCGCCGGGTCACGCCGACTACATCAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCCATCCTGGT
GGTCGCCGCGACCGACGGCCCGATGCCGCAGACCCGCGAGCACGTGCTGCTGGCCCGCCAGGTCGGCGTGCCCTACATCC
TGGTCGCCCTGAACAAGGCCGACATGGTCGACGACGAGGAGATCCTCGAGCTCGTCGAGATGGAGGTCCGCGAGCTGCTG
GCCGCCCAGGAGTTCGACGAGGAAGCCCCCGTCGTGCGCGTGTCCGGCCTGAAGGCCCTCGAGGGCGACCCCAAGTGGGT
CAAGTCCGTCGAGGACCTGATGGACGCCGTCGACGAGTCCATCCCGGACCCGGTGCGTGAGACCGACAAGCCGTTCCTCA
TGCCGATCGAGGACGTCTTCACCATCACCGGCCGTGGCACCGTCGTCACCGGTCGTGTCGAGCGCGGCATCATCAACGTG
AACGAGGAAGTGGAGATCACCGGCATCCGTCCGGAGACCACCAAGACCACGGTCACCGGTATCGAGATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCGGGCGACAACGTCGGCCTGCTGATCCGTGGCATCAAGCGCGAGGACGTGGAGCGCGGCCAGG
TCGTCATCAAGCCGGGCACCACCACCCCGCACACCGAGTTCGAGGGCCAGGCGTACATCCTGTCGAAGGACGAGGGCGGC
CGCCACACCCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGCGTCGTGACCCTCCC
CGAGGGCACCGAGATGGTCATGCCGGGCGACAACACCGAGATGAGCGTCAAGCTGATCCAGCCGGTCGCCATGGAAGAGG
GCCTGCGCTTCGCGATCCGCGAGGGTGGCCGCACCGTCGGTGCCGGCCGCGTCACCAAGATCATCAAGTGA

Upstream 100 bases:

>100_bases
CCGGGCGCTCTGTACGACCCGAAATTTCCCTGCATCCGCAGGGATGACCCCCCGCACTGCTGCAACTGCAGGAACCAACA
CAGTCCAGGAGGACAAAACA

Downstream 100 bases:

>100_bases
TTTCCTGAAATCACGATCTGCCACGGCGCCGCTCCGCAAGGGGCGGCGCCGTTCGGCGTTTCCGGGGGCCGTCCTGATTT
CGGTCCACTGCCGATTGCTG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLADKYPDLNQSFAFDQIDKAPEEKARGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADMVDDEEILELVEMEVRELL
AAQEFDEEAPVVRVSGLKALEGDPKWVKSVEDLMDAVDESIPDPVRETDKPFLMPIEDVFTITGRGTVVTGRVERGIINV
NEEVEITGIRPETTKTTVTGIEMFRKLLDQGQAGDNVGLLIRGIKREDVERGQVVIKPGTTTPHTEFEGQAYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMSVKLIQPVAMEEGLRFAIREGGRTVGAGRVTKIIK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLADKYPDLNQSFAFDQIDKAPEEKARGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADMVDDEEILELVEMEVRELL
AAQEFDEEAPVVRVSGLKALEGDPKWVKSVEDLMDAVDESIPDPVRETDKPFLMPIEDVFTITGRGTVVTGRVERGIINV
NEEVEITGIRPETTKTTVTGIEMFRKLLDQGQAGDNVGLLIRGIKREDVERGQVVIKPGTTTPHTEFEGQAYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMSVKLIQPVAMEEGLRFAIREGGRTVGAGRVTKIIK
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLADKYPDLNQSFAFDQIDKAPEEKARGITINISHVEYQTEKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADMVDDEEILELVEMEVRELLA
AQEFDEEAPVVRVSGLKALEGDPKWVKSVEDLMDAVDESIPDPVRETDKPFLMPIEDVFTITGRGTVVTGRVERGIINVN
EEVEITGIRPETTKTTVTGIEMFRKLLDQGQAGDNVGLLIRGIKREDVERGQVVIKPGTTTPHTEFEGQAYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMSVKLIQPVAMEEGLRFAIREGGRTVGAGRVTKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=56.0301507537689, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI4503475, Length=350, Percent_Identity=34.8571428571429, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4503471, Length=455, Percent_Identity=30.7692307692308, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=31.2925170068027, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=31.2925170068027, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI194018522, Length=441, Percent_Identity=25.1700680272109, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI194018520, Length=441, Percent_Identity=25.1700680272109, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194097354, Length=441, Percent_Identity=25.1700680272109, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI46094014, Length=325, Percent_Identity=28.6153846153846, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI54607086, Length=326, Percent_Identity=26.9938650306748, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4503507, Length=292, Percent_Identity=26.7123287671233, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI82546879, Length=436, Percent_Identity=22.9357798165138, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1790412, Length=397, Percent_Identity=74.3073047858942, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=74.4949494949495, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=32.7433628318584, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789108, Length=328, Percent_Identity=27.7439024390244, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI48994988, Length=285, Percent_Identity=30.8771929824561, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=404, Percent_Identity=48.7623762376238, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=45.4545454545455, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=328, Percent_Identity=35.9756097560976, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=328, Percent_Identity=35.9756097560976, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=318, Percent_Identity=33.0188679245283, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=31.5018315018315, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=453, Percent_Identity=24.5033112582781, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=43.2432432432432, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=296, Percent_Identity=27.027027027027, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=256, Percent_Identity=27.734375, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=63, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.8558558558559, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.8558558558559, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=354, Percent_Identity=26.5536723163842, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=327, Percent_Identity=30.8868501529052, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=277, Percent_Identity=26.7148014440433, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=237, Percent_Identity=27.4261603375527, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=57.3232323232323, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=57.3232323232323, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=50.8997429305913, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=31.0810810810811, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=31.0810810810811, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=31.0810810810811, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=31.0810810810811, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24652838, Length=443, Percent_Identity=30.0225733634311, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137572, Length=443, Percent_Identity=30.0225733634311, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=29.1954022988506, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.8196721311475, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=319, Percent_Identity=30.0940438871473, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647048, Length=289, Percent_Identity=24.5674740484429, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647046, Length=289, Percent_Identity=24.5674740484429, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=169, Percent_Identity=36.094674556213, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43770; Mature: 43638

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLADKYPDLNQSFAFDQIDKAPEEKA
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CEEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADMVDDEEILELVEMEVRELLAAQEFDEEAPVVRVSGLKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEC
EGDPKWVKSVEDLMDAVDESIPDPVRETDKPFLMPIEDVFTITGRGTVVTGRVERGIINV
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHEEEEECCCEEEEEECCCCEEEC
NEEVEITGIRPETTKTTVTGIEMFRKLLDQGQAGDNVGLLIRGIKREDVERGQVVIKPGT
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEECCC
TTPHTEFEGQAYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MSVKLIQPVAMEEGLRFAIREGGRTVGAGRVTKIIK
EEEEEECCHHHHCCCEEEECCCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLADKYPDLNQSFAFDQIDKAPEEKA
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CEEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADMVDDEEILELVEMEVRELLAAQEFDEEAPVVRVSGLKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEC
EGDPKWVKSVEDLMDAVDESIPDPVRETDKPFLMPIEDVFTITGRGTVVTGRVERGIINV
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHEEEEECCCEEEEEECCCCEEEC
NEEVEITGIRPETTKTTVTGIEMFRKLLDQGQAGDNVGLLIRGIKREDVERGQVVIKPGT
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEECCC
TTPHTEFEGQAYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MSVKLIQPVAMEEGLRFAIREGGRTVGAGRVTKIIK
EEEEEECCHHHHCCCEEEECCCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA