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The map label for this gene is ushA

Identifier: 54020636

GI number: 54020636

Start: 822354

End: 824498

Strand: Reverse

Name: ushA

Synonym: mhp651

Alternate gene names: 54020636

Gene position: 824498-822354 (Counterclockwise)

Preceding gene: 54020637

Following gene: 54020635

Centisome position: 92.35

GC content: 32.82

Gene sequence:

>2145_bases
TTGAAGAAGAAACTTTTATTACCTTTTTGCACTATTGCAACTTTTATTACCGGAATTCCTTTAATTGTTAGTGCTGCTTG
TAGTGAAAACGCTACTCAGGTTACAAAAAATACTCAAAAAATAACTGAAAATAGCGCTCAAGTTAAAGAAAATTCTGAAA
AGATTAGGGAAGAATATTTTTCTGTAGTTGAGAAATATTCGGATACCTTAAATGATTTCCAAGCAAAAATAGACAAAATC
CGTAAAACTAATCCAAAAAGTGATACAGATGAAATCGCTATAATTATTAGCGAAGCCCAAGCCAAAATTGATCCCCTAAT
TGATCAGGTCAATTATCTTTTTAAAAAATTGCATGAATTAGAAAAAAAAGAAAATTCAAAACTTAAAACTGTAAAAATTT
TTCATACAAACGACGAACACGGTCGCTTAGAATATGATGATAACAAACACAATAATTATTCAGGGATGGATAAAACGGGA
CAGTACTTGAAAAAATTTAACAAGGATTTACTTTTATCTGCCGGAGATTTAATTCAAGGACTTCCACTTTCGGATACAGA
TAAAGGTAAGACAATTGCAAAAATTGCCAAATATATAGGCTATGATTCAGTTGCGATTGGAAACCATGAATTCGACTATG
GAATTAGACACATTCTTGATCTTGCCGCCGAGGGAAACAAAGATGAATTTAGCCGAAAAATGCCTTTTATTTCCGCTAAT
GTTTACTGAAGACAACCTTCAGAAGAGGAACTTAAAAATTTAGAATCTACAGATGAAAGAGCCATAAAACCAGCTGAATT
TAAACAAGGGAAGCGAGTTTTCCAACCTTATATAATAAAGGAATTAGAAAATGGTCTAAAAGTGGCAGTTATCGGGCTGA
CAACTCCAGATACAAAAATTACTTCCCACCCAAAAAATTCTTTTTGAGTTGAATTTACCGATCCAGTACCTGAAACCCAA
AAGGTAATTAAGGAGATTCAGGAAAAAGATCCTTCAATTTCCTTTATAATTGCTACAACGCACCTAGGTGTTGGGCGCAC
AGAACATAAATGAACTTCAGATTATCTTGCAGAAGAAACCGGAAATGGCCTTGATTTAGTCATCGATGGGCATTCGCATA
CAAAAATCGAAATTCACAAACCCAAAGAAGATAAAAAAGTTTGGGTAACCCAAACTGAGGCCTATGCAAAATGGCTCGGG
GACATTGATTTAGTTTTTGATACTGAAACTGGTGAGATAGTAAAAATTGTCCAATCTTTAAGAGATATTAACCAAATTAA
TATTGTAACCAGAGATTTATCAGAACATTATATCAAAAAACTTCATAAAGTTTATGATGTAGAAAATGATGTAAAAGTAT
TTAATTCGCCTGGTGTTTTTGAACATGTTCAATCAATTGAGATCAACAAAACTCCTTACTGAATTGGACGGGTAAAACCA
ACTTCATTAGGGGTAATGACAGCAGATGCCATTGCTTGGGAATATGCAAAAATTTCAGAAGAACAAGTACAAAGTACGAA
AAATGAAATTGCAACACTTGATAATTCCTTAGGACTTATAAACGGTGGTAGTCTTAGAACAGATCTAAAAAGTGGTGAAA
TCAAAAGAGGAGATGTTTTAGGGGTTAGTCCTTTTGGGAATAGGATTGTAACTATTAAACTAAAAGGAGATACACTTAAA
AAAACTCTCGAATACGGGCTTTCTATGGGAAAACAAGGCGGATTTGCCCAACTATCTTCAAATATTTCCTATAAAGTCAA
AGTTGAAAAAGGGACTGATCCGAAAACCAAAATAGAATCCTGGGTTTGAAAACCAGATACAACATCCTTTAAAATTAATA
ACAAGCCGATTGATGATAACAAATTTTACTATTTAAGTACAAATGATTATCTTTCAGCGGGCGGCGACGGCTATCAAATG
CTAAATCTAGGTAAAAACAAGGATATTGAAAAAGTTTATGAGGGAGTTCAATATATTAATTCTTTAATTAAATATGGTCA
ATATTTGGAGAAATTAACTAAAGATTCAACTCAAAAAGATCTATTTGCTCACACATTCCAAGAATATTTAAGTTCTGATT
TTACAAAAAATCAACAAGTTGATATTCCTCAAGAAGCTCTAACGAAAAGCCAAAGTCAATCTTAA

Upstream 100 bases:

>100_bases
ATTCATATACAAAGTAAGGAAGAAAAATTTTAAGAAATTTTCTTCTTTTTTAAGGCGTTTAAGTTATAATACAATTTTAA
CAAAAAATAAGAGGAGAAAA

Downstream 100 bases:

>100_bases
TTATTTTAAATTTTTTAAAAAGACACACTTTTGAGTGTGTTTTTTTATTTTTTTACCTTTTTTTGCAAAAAAAAAAAAAA
AAGGTTAAAATTAAAAAGAA

Product: 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKKKLLLPFCTIATFITGIPLIVSAACSENATQVTKNTQKITENSAQVKENSEKIREEYFSVVEKYSDTLNDFQAKIDKI
RKTNPKSDTDEIAIIISEAQAKIDPLIDQVNYLFKKLHELEKKENSKLKTVKIFHTNDEHGRLEYDDNKHNNYSGMDKTG
QYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFDYGIRHILDLAAEGNKDEFSRKMPFISAN
VYWRQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIGLTTPDTKITSHPKNSFWVEFTDPVPETQ
KVIKEIQEKDPSISFIIATTHLGVGRTEHKWTSDYLAEETGNGLDLVIDGHSHTKIEIHKPKEDKKVWVTQTEAYAKWLG
DIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSEHYIKKLHKVYDVENDVKVFNSPGVFEHVQSIEINKTPYWIGRVKP
TSLGVMTADAIAWEYAKISEEQVQSTKNEIATLDNSLGLINGGSLRTDLKSGEIKRGDVLGVSPFGNRIVTIKLKGDTLK
KTLEYGLSMGKQGGFAQLSSNISYKVKVEKGTDPKTKIESWVWKPDTTSFKINNKPIDDNKFYYLSTNDYLSAGGDGYQM
LNLGKNKDIEKVYEGVQYINSLIKYGQYLEKLTKDSTQKDLFAHTFQEYLSSDFTKNQQVDIPQEALTKSQSQS

Sequences:

>Translated_714_residues
MKKKLLLPFCTIATFITGIPLIVSAACSENATQVTKNTQKITENSAQVKENSEKIREEYFSVVEKYSDTLNDFQAKIDKI
RKTNPKSDTDEIAIIISEAQAKIDPLIDQVNYLFKKLHELEKKENSKLKTVKIFHTNDEHGRLEYDDNKHNNYSGMDKTG
QYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFDYGIRHILDLAAEGNKDEFSRKMPFISAN
VY*RQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIGLTTPDTKITSHPKNSF*VEFTDPVPETQ
KVIKEIQEKDPSISFIIATTHLGVGRTEHK*TSDYLAEETGNGLDLVIDGHSHTKIEIHKPKEDKKVWVTQTEAYAKWLG
DIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSEHYIKKLHKVYDVENDVKVFNSPGVFEHVQSIEINKTPY*IGRVKP
TSLGVMTADAIAWEYAKISEEQVQSTKNEIATLDNSLGLINGGSLRTDLKSGEIKRGDVLGVSPFGNRIVTIKLKGDTLK
KTLEYGLSMGKQGGFAQLSSNISYKVKVEKGTDPKTKIESWV*KPDTTSFKINNKPIDDNKFYYLSTNDYLSAGGDGYQM
LNLGKNKDIEKVYEGVQYINSLIKYGQYLEKLTKDSTQKDLFAHTFQEYLSSDFTKNQQVDIPQEALTKSQSQS
>Mature_714_residues
MKKKLLLPFCTIATFITGIPLIVSAACSENATQVTKNTQKITENSAQVKENSEKIREEYFSVVEKYSDTLNDFQAKIDKI
RKTNPKSDTDEIAIIISEAQAKIDPLIDQVNYLFKKLHELEKKENSKLKTVKIFHTNDEHGRLEYDDNKHNNYSGMDKTG
QYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFDYGIRHILDLAAEGNKDEFSRKMPFISAN
VY*RQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIGLTTPDTKITSHPKNSF*VEFTDPVPETQ
KVIKEIQEKDPSISFIIATTHLGVGRTEHK*TSDYLAEETGNGLDLVIDGHSHTKIEIHKPKEDKKVWVTQTEAYAKWLG
DIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSEHYIKKLHKVYDVENDVKVFNSPGVFEHVQSIEINKTPY*IGRVKP
TSLGVMTADAIAWEYAKISEEQVQSTKNEIATLDNSLGLINGGSLRTDLKSGEIKRGDVLGVSPFGNRIVTIKLKGDTLK
KTLEYGLSMGKQGGFAQLSSNISYKVKVEKGTDPKTKIESWV*KPDTTSFKINNKPIDDNKFYYLSTNDYLSAGGDGYQM
LNLGKNKDIEKVYEGVQYINSLIKYGQYLEKLTKDSTQKDLFAHTFQEYLSSDFTKNQQVDIPQEALTKSQSQS

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=557, Percent_Identity=24.7755834829443, Blast_Score=93, Evalue=9e-19,
Organism=Escherichia coli, GI1786687, Length=556, Percent_Identity=25.5395683453237, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922444, Length=559, Percent_Identity=25.2236135957066, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922446, Length=572, Percent_Identity=24.8251748251748, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654424, Length=572, Percent_Identity=24.8251748251748, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573524, Length=550, Percent_Identity=23.8181818181818, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 79915; Mature: 79915

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLLLPFCTIATFITGIPLIVSAACSENATQVTKNTQKITENSAQVKENSEKIREEYF
CCCCEECHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVVEKYSDTLNDFQAKIDKIRKTNPKSDTDEIAIIISEAQAKIDPLIDQVNYLFKKLHEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
EKKENSKLKTVKIFHTNDEHGRLEYDDNKHNNYSGMDKTGQYLKKFNKDLLLSAGDLIQG
HHHCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCHHHEEECCHHHCC
LPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFDYGIRHILDLAAEGNKDEFSRKMPFISAN
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEECC
VYRQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIGLTTPDTKIT
CCCCCCHHHHHCCCCCHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
SHPKNSFVEFTDPVPETQKVIKEIQEKDPSISFIIATTHLGVGRTEHKTSDYLAEETGNG
CCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCC
LDLVIDGHSHTKIEIHKPKEDKKVWVTQTEAYAKWLGDIDLVFDTETGEIVKIVQSLRDI
EEEEEECCCCCEEEEECCCCCCEEEEEEHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCC
NQINIVTRDLSEHYIKKLHKVYDVENDVKVFNSPGVFEHVQSIEINKTPYIGRVKPTSLG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHEECCCCCCCCCCCCCCCC
VMTADAIAWEYAKISEEQVQSTKNEIATLDNSLGLINGGSLRTDLKSGEIKRGDVLGVSP
EEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCEECCCCCCCCCCCEEEECC
FGNRIVTIKLKGDTLKKTLEYGLSMGKQGGFAQLSSNISYKVKVEKGTDPKTKIESWVKP
CCCEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHCCC
DTTSFKINNKPIDDNKFYYLSTNDYLSAGGDGYQMLNLGKNKDIEKVYEGVQYINSLIKY
CCCEEEECCCCCCCCCEEEEECCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
GQYLEKLTKDSTQKDLFAHTFQEYLSSDFTKNQQVDIPQEALTKSQSQS
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MKKKLLLPFCTIATFITGIPLIVSAACSENATQVTKNTQKITENSAQVKENSEKIREEYF
CCCCEECHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVVEKYSDTLNDFQAKIDKIRKTNPKSDTDEIAIIISEAQAKIDPLIDQVNYLFKKLHEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
EKKENSKLKTVKIFHTNDEHGRLEYDDNKHNNYSGMDKTGQYLKKFNKDLLLSAGDLIQG
HHHCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCHHHEEECCHHHCC
LPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFDYGIRHILDLAAEGNKDEFSRKMPFISAN
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEECC
VYRQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIGLTTPDTKIT
CCCCCCHHHHHCCCCCHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
SHPKNSFVEFTDPVPETQKVIKEIQEKDPSISFIIATTHLGVGRTEHKTSDYLAEETGNG
CCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCC
LDLVIDGHSHTKIEIHKPKEDKKVWVTQTEAYAKWLGDIDLVFDTETGEIVKIVQSLRDI
EEEEEECCCCCEEEEECCCCCCEEEEEEHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCC
NQINIVTRDLSEHYIKKLHKVYDVENDVKVFNSPGVFEHVQSIEINKTPYIGRVKPTSLG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHEECCCCCCCCCCCCCCCC
VMTADAIAWEYAKISEEQVQSTKNEIATLDNSLGLINGGSLRTDLKSGEIKRGDVLGVSP
EEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCEECCCCCCCCCCCEEEECC
FGNRIVTIKLKGDTLKKTLEYGLSMGKQGGFAQLSSNISYKVKVEKGTDPKTKIESWVKP
CCCEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHCCC
DTTSFKINNKPIDDNKFYYLSTNDYLSAGGDGYQMLNLGKNKDIEKVYEGVQYINSLIKY
CCCEEEECCCCCCCCCEEEEECCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
GQYLEKLTKDSTQKDLFAHTFQEYLSSDFTKNQQVDIPQEALTKSQSQS
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]