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The map label for this gene is nusA

Identifier: 54020616

GI number: 54020616

Start: 753833

End: 755677

Strand: Reverse

Name: nusA

Synonym: mhp603

Alternate gene names: 54020616

Gene position: 755677-753833 (Counterclockwise)

Preceding gene: 54020617

Following gene: 54020615

Centisome position: 84.65

GC content: 30.41

Gene sequence:

>1845_bases
ATGAATAGAAAAAAAACCGAAAAATCAAAAATTAGTTCAAAAGATAGTAAAAAATTAATAATTCAAGCTATCCAAGATGT
GGCAAAAAATAGCGAATTAAATCTGGAAGCAGTTATCGATATTTTTCAGGAAGCAATTGAATTTGTAATAACAAAGAAAA
TTGACCCAGATGCTCAAATAAAAATTGAAGCTGATCTTGAACAATTGAGTTTTAAAGTTTTTAATACAAACGGGATTGTT
GTTGAAGAAAATTATTTTGATGATCTTACAGATGAGGAAAAAGTTAACGATCTTGTTTCTTTTATTTTACTATCAAAGGC
AAAAGAAACTGATCCTGAGATTCAGGTTGATGATATTTTTTCAATTGAAATTAATCTTGAAAGTTTTGAACATTGACTTT
TTATGGCAATTATGCACGCTTTTAAGCAAAAAATTTCTGAAATTGTCCGAAATAATGTTTATAACAAATATTTATCGCTT
AAAAATAACGTAGTTTTGGCCACTGTTACTAATAAAATTGCTGCTGGTTATATTTTCGAAATTGACGATGATAAAGTTTC
TGCCTTCATGCCAAGCCATTATGCAAGTGGACAAAATTTAAAAATAGGCACTAAACATGAAGTTGTAATTGAAAATGTAT
CAAAAAATACAAAACAGTCCCAGGTTGTGATATCCTCAAAATCAGTTCAACTTGTCAAGAAAAAAATAATCGATGCAATC
CCTGAACTACAGTCAAAATTTCTTGAAATCACTTCAATTGCGCGGATTCCAGGGGAAAGATGCAAAGTCGCAATTCGCAG
AACTGAGGGTGCCGAGGCTGATAATATTTCTGAAATCGGCTCAATTGTAGGAGCAACTGGCTCAAGAGTTCTTGCAATTT
CTCAAGAGCTTCAGGGTGAAAAAATTGAGGTGATTAAATATGATGACAATATTGTCAAATTTATTGTTAATGCGATGTCG
CCTTCAAAAGTTATTTGCGTAAAAGAGTTCAAAATAGGTCATAAATTACGTCGTTTTATCGTAGTTGTTACTGATTTTCA
ACATAGTTTAGCCATTGGAAAAAACGGTTCAAATGTTAAACTAGTAGCAGATCTAACACGTTGTCAAGTGCAAATTATCC
CATATTCTAGCGCACTAAAAGATCAAAATTTTAAAATTGAATGAAATGGAAATATTAAAGACATTCAAGAACTAAACAGT
CTTAAAAATGAGTATATTCATCGCCAGCAAGGTCGAATTTATCAGAACCATCGAAATTCATATGGTCAAGGTAATAATAA
TTTTGATTTAATTTTACAGCAATTCGAATCTGATATGCGTGAGTTAGAAAAACCTTATGGGATTGAAAAAGAGTTTGTTC
CAAAAAATGAGCAAAAACAGGTAAGAGATCACCAAGAATTTCCAAAAAACAAAAATAATCTAACAAAAGCAGGAACTAAA
TCCCGTAATTTTAACAAAAGCCAGAATATCCGCGAAAATATTTCAAAAGATTTTGATTATGGCTTTGAGAGGGAGAAAGA
TTCTAATTCTTTCCAAAATATTAGTCAAAGATCTTTTTTTGATGCAGATTCACTTTTTGATTCAGCTCTAAATGAGGCAA
TTAGTGAAAACGAGTTAATCGATAAAATTCACCAAGAAGAGGAAAAAAAACAAGAATTATTATTACAAGAGAAGGAAAAA
TGAGCAAAAAATGAAGCTCAAATCCAAAAAGCTAGTCAAGAACCTAACCAAAATTCAGATTTATATATCCAAAATGAAAA
ACAAATAAAAAATTTCAAATTAGATGATGATCTTCTAAAATATACAGGGGTTGACGATATTGACATTGATGATTTTGATT
TCTAG

Upstream 100 bases:

>100_bases
GAGGAAATTTCAAAAGAAATTTCCATTTTTATCGATAAAATTGAGACCGATGAGAAAAATTTTATTTTAGAAGTACTTTC
AAAAGGGCAAGATTTCGAAA

Downstream 100 bases:

>100_bases
GATAAAAAAGTGAAAAATTATACAAGAAAATGTATTGTTGATCAAAAAATTTATCCAGTAAGTGAATTAATAAGATTTGT
TTATATTGATAAAAAGTTAG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQIKIEADLEQLSFKVFNTNGIV
VEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIFSIEINLESFEHWLFMAIMHAFKQKISEIVRNNVYNKYLSL
KNNVVLATVTNKIAAGYIFEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIIDAI
PELQSKFLEITSIARIPGERCKVAIRRTEGAEADNISEIGSIVGATGSRVLAISQELQGEKIEVIKYDDNIVKFIVNAMS
PSKVICVKEFKIGHKLRRFIVVVTDFQHSLAIGKNGSNVKLVADLTRCQVQIIPYSSALKDQNFKIEWNGNIKDIQELNS
LKNEYIHRQQGRIYQNHRNSYGQGNNNFDLILQQFESDMRELEKPYGIEKEFVPKNEQKQVRDHQEFPKNKNNLTKAGTK
SRNFNKSQNIRENISKDFDYGFEREKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDKIHQEEEKKQELLLQEKEK
WAKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTGVDDIDIDDFDF

Sequences:

>Translated_614_residues
MNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQIKIEADLEQLSFKVFNTNGIV
VEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIFSIEINLESFEH*LFMAIMHAFKQKISEIVRNNVYNKYLSL
KNNVVLATVTNKIAAGYIFEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIIDAI
PELQSKFLEITSIARIPGERCKVAIRRTEGAEADNISEIGSIVGATGSRVLAISQELQGEKIEVIKYDDNIVKFIVNAMS
PSKVICVKEFKIGHKLRRFIVVVTDFQHSLAIGKNGSNVKLVADLTRCQVQIIPYSSALKDQNFKIE*NGNIKDIQELNS
LKNEYIHRQQGRIYQNHRNSYGQGNNNFDLILQQFESDMRELEKPYGIEKEFVPKNEQKQVRDHQEFPKNKNNLTKAGTK
SRNFNKSQNIRENISKDFDYGFEREKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDKIHQEEEKKQELLLQEKEK
*AKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTGVDDIDIDDFDF
>Mature_614_residues
MNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQIKIEADLEQLSFKVFNTNGIV
VEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIFSIEINLESFEH*LFMAIMHAFKQKISEIVRNNVYNKYLSL
KNNVVLATVTNKIAAGYIFEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIIDAI
PELQSKFLEITSIARIPGERCKVAIRRTEGAEADNISEIGSIVGATGSRVLAISQELQGEKIEVIKYDDNIVKFIVNAMS
PSKVICVKEFKIGHKLRRFIVVVTDFQHSLAIGKNGSNVKLVADLTRCQVQIIPYSSALKDQNFKIE*NGNIKDIQELNS
LKNEYIHRQQGRIYQNHRNSYGQGNNNFDLILQQFESDMRELEKPYGIEKEFVPKNEQKQVRDHQEFPKNKNNLTKAGTK
SRNFNKSQNIRENISKDFDYGFEREKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDKIHQEEEKKQELLLQEKEK
*AKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTGVDDIDIDDFDF

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=358, Percent_Identity=22.9050279329609, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 69949; Mature: 69949

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQI
CCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
KIEADLEQLSFKVFNTNGIVVEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIF
EEEECHHHCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCEE
SIEINLESFEHLFMAIMHAFKQKISEIVRNNVYNKYLSLKNNVVLATVTNKIAAGYIFEI
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCEEEEE
DDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIIDAIP
CCCCHHHHCCHHCCCCCCEECCCCCHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHH
ELQSKFLEITSIARIPGERCKVAIRRTEGAEADNISEIGSIVGATGSRVLAISQELQGEK
HHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEHHCCCCE
IEVIKYDDNIVKFIVNAMSPSKVICVKEFKIGHKLRRFIVVVTDFQHSLAIGKNGSNVKL
EEEEEECCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHEEEEECCCCCEEECCCCCCEEE
VADLTRCQVQIIPYSSALKDQNFKIENGNIKDIQELNSLKNEYIHRQQGRIYQNHRNSYG
EEEECCCEEEEEECHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
QGNNNFDLILQQFESDMRELEKPYGIEKEFVPKNEQKQVRDHQEFPKNKNNLTKAGTKSR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
NFNKSQNIRENISKDFDYGFEREKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDK
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IHQEEEKKQELLLQEKEKAKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCHHHHHHCC
VDDIDIDDFDF
CCCCCCCCCCC
>Mature Secondary Structure
MNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQI
CCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
KIEADLEQLSFKVFNTNGIVVEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIF
EEEECHHHCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCEE
SIEINLESFEHLFMAIMHAFKQKISEIVRNNVYNKYLSLKNNVVLATVTNKIAAGYIFEI
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCEEEEE
DDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIIDAIP
CCCCHHHHCCHHCCCCCCEECCCCCHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHH
ELQSKFLEITSIARIPGERCKVAIRRTEGAEADNISEIGSIVGATGSRVLAISQELQGEK
HHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEHHCCCCE
IEVIKYDDNIVKFIVNAMSPSKVICVKEFKIGHKLRRFIVVVTDFQHSLAIGKNGSNVKL
EEEEEECCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHEEEEECCCCCEEECCCCCCEEE
VADLTRCQVQIIPYSSALKDQNFKIENGNIKDIQELNSLKNEYIHRQQGRIYQNHRNSYG
EEEECCCEEEEEECHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
QGNNNFDLILQQFESDMRELEKPYGIEKEFVPKNEQKQVRDHQEFPKNKNNLTKAGTKSR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
NFNKSQNIRENISKDFDYGFEREKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDK
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IHQEEEKKQELLLQEKEKAKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCHHHHHHCC
VDDIDIDDFDF
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]