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The map label for this gene is bkdA1

Identifier: 53723291

GI number: 53723291

Start: 3054057

End: 3055289

Strand: Reverse

Name: bkdA1

Synonym: BPSS2273

Alternate gene names: 53723291

Gene position: 3055289-3054057 (Counterclockwise)

Preceding gene: 53723293

Following gene: 53723290

Centisome position: 96.29

GC content: 67.56

Gene sequence:

>1233_bases
ATGAGCCAGTACGGGCCATTGCGATTGCATGTGCCGGAGCCTACCGGGCGTCCGGGATGCAAGACCGATTTTTCTTACCT
GCGCCTGTCGCCAGCCGGCAAGGTGCGCAAACCCCCCATCGACGTCGCCCCCGCCGACACGAGCGACTTGGCCTATGGCC
TCGTGCGCGTGCTCGACGAGCACGGACGAGCGGTCGGGCCATGGGCGCCCGACCTCGATCCCGACATCTTGCGCAAAGGC
ATCCGCGCGATGCTGAAGACGCGCATCTTCGACGCGCGCATGCAGATCGCGCAGCGCCAGAAGAAGATCTCGTTCTACAT
GCAGTGCCTCGGCGAGGAAGCGATCGCGGTCGCGCACACGCTCGCGCTCGAGCGCGGCGACATGTGCTTTCCGACGTATC
GGCAGCAAGGGATCCTGATGGTGCGCGAGTATCCGCTCGTCGACATGATGTGCCAGCTGATGTCGAACGAGCGCGATCCG
CTGAAGGGCCGCCAGTTGCCGGTGATGTACTCGACGCGCGACGCGGGCTTCTTCTCGATCTCGGGCAATCTCGCGACGCA
GTTCATCCAGGCGGTCGGCTGGGCGATGGCCTCGGCGATCAAGGGCGACACGCGCATCGCTTCCGCGTGGATCGGCGACG
GCGCGACGGCCGAAGCCGATTTCCATACCGCGCTCACGTTCGCACACGTGTACCGCGCGCCCGTGATCCTGAACGTGGTC
AACAACCAGTGGGCGATCTCGACGTTCCAGGCGATCGCGGGCGGCGAGGGCGCGACCTTCGCCGGGCGCGGCGTCGGCTG
CGGCATCGCTTCGCTGCGCGTGGACGGCAACGATTTCCTCGCGGTCTACGCCGCGTCCCGCTGGGCGGCCGAACGCGCGC
GCCGCAACCTCGGGCCGACGCTGATCGAGTGGGTCACGTACCGCGCGGGCCCGCACTCGACGTCCGACGATCCGACCAAG
TACCGCCCCGGCGACGACTGGACGAACTTTCCGCTCGGCGATCCGCTCGAGCGCCTGAAGCGCCACATGATCGGCATCGG
CGTGTGGTCCGAGCAGGACCACGAGGACACGAAGGCCGCGCTCGAGGCCGAGGTGCTCGCCGCGCAGAAGGAAGCGGAGC
GCTACGGCACGCTGGCCGACGAGCACGTGCCGAACGTCGCGAGCATTTTCGAGGACGTCTACAAGGAGATGCCCGCGCAC
CTGCGCCGGCAGCGCCAACAGCTCGGGGTTTGA

Upstream 100 bases:

>100_bases
TCGGGGCGACTCCGAGTCCGAAGAAGCTTTCACGATGCGCGATAACTTTGTTACGCTCTGCGACGAAGCGTGCGACAAAA
TCAAACGTGGAGACAATTGA

Downstream 100 bases:

>100_bases
CAGCGATGACGACAGCAAGCAAAGAAGGTCCGGCGGCCTCGCCGATGACCATGATCCAGGCTTTGCGCTCGGCGATGGAC
GTGATGCTCGAGCGCGACGG

Product: 2-oxoisovalerate dehydrogenase alpha subunit

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSQYGPLRLHVPEPTGRPGCKTDFSYLRLSPAGKVRKPPIDVAPADTSDLAYGLVRVLDEHGRAVGPWAPDLDPDILRKG
IRAMLKTRIFDARMQIAQRQKKISFYMQCLGEEAIAVAHTLALERGDMCFPTYRQQGILMVREYPLVDMMCQLMSNERDP
LKGRQLPVMYSTRDAGFFSISGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPTLIEWVTYRAGPHSTSDDPTK
YRPGDDWTNFPLGDPLERLKRHMIGIGVWSEQDHEDTKAALEAEVLAAQKEAERYGTLADEHVPNVASIFEDVYKEMPAH
LRRQRQQLGV

Sequences:

>Translated_410_residues
MSQYGPLRLHVPEPTGRPGCKTDFSYLRLSPAGKVRKPPIDVAPADTSDLAYGLVRVLDEHGRAVGPWAPDLDPDILRKG
IRAMLKTRIFDARMQIAQRQKKISFYMQCLGEEAIAVAHTLALERGDMCFPTYRQQGILMVREYPLVDMMCQLMSNERDP
LKGRQLPVMYSTRDAGFFSISGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPTLIEWVTYRAGPHSTSDDPTK
YRPGDDWTNFPLGDPLERLKRHMIGIGVWSEQDHEDTKAALEAEVLAAQKEAERYGTLADEHVPNVASIFEDVYKEMPAH
LRRQRQQLGV
>Mature_409_residues
SQYGPLRLHVPEPTGRPGCKTDFSYLRLSPAGKVRKPPIDVAPADTSDLAYGLVRVLDEHGRAVGPWAPDLDPDILRKGI
RAMLKTRIFDARMQIAQRQKKISFYMQCLGEEAIAVAHTLALERGDMCFPTYRQQGILMVREYPLVDMMCQLMSNERDPL
KGRQLPVMYSTRDAGFFSISGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVVN
NQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPTLIEWVTYRAGPHSTSDDPTKY
RPGDDWTNFPLGDPLERLKRHMIGIGVWSEQDHEDTKAALEAEVLAAQKEAERYGTLADEHVPNVASIFEDVYKEMPAHL
RRQRQQLGV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=357, Percent_Identity=39.2156862745098, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI258645172, Length=358, Percent_Identity=39.1061452513966, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI4885543, Length=289, Percent_Identity=25.9515570934256, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI291084742, Length=290, Percent_Identity=26.551724137931, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI4505685, Length=290, Percent_Identity=26.551724137931, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI291084744, Length=258, Percent_Identity=26.3565891472868, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI86563357, Length=342, Percent_Identity=39.4736842105263, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI86563355, Length=342, Percent_Identity=39.4736842105263, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17536047, Length=304, Percent_Identity=25.6578947368421, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32564172, Length=304, Percent_Identity=25.6578947368421, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321026, Length=344, Percent_Identity=29.6511627906977, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355903, Length=361, Percent_Identity=36.01108033241, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24639748, Length=324, Percent_Identity=24.3827160493827, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24639744, Length=286, Percent_Identity=25.1748251748252, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28571106, Length=286, Percent_Identity=25.1748251748252, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24639746, Length=288, Percent_Identity=25, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24639740, Length=288, Percent_Identity=25, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 45416; Mature: 45285

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYGPLRLHVPEPTGRPGCKTDFSYLRLSPAGKVRKPPIDVAPADTSDLAYGLVRVLDE
CCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HGRAVGPWAPDLDPDILRKGIRAMLKTRIFDARMQIAQRQKKISFYMQCLGEEAIAVAHT
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALERGDMCFPTYRQQGILMVREYPLVDMMCQLMSNERDPLKGRQLPVMYSTRDAGFFSI
HHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEE
SGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
CCHHHHHHHHHHHHHHHHHHCCCHHEEHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEC
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPT
CCCHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCCCHH
LIEWVTYRAGPHSTSDDPTKYRPGDDWTNFPLGDPLERLKRHMIGIGVWSEQDHEDTKAA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
LEAEVLAAQKEAERYGTLADEHVPNVASIFEDVYKEMPAHLRRQRQQLGV
HHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQYGPLRLHVPEPTGRPGCKTDFSYLRLSPAGKVRKPPIDVAPADTSDLAYGLVRVLDE
CCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HGRAVGPWAPDLDPDILRKGIRAMLKTRIFDARMQIAQRQKKISFYMQCLGEEAIAVAHT
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALERGDMCFPTYRQQGILMVREYPLVDMMCQLMSNERDPLKGRQLPVMYSTRDAGFFSI
HHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEE
SGNLATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVV
CCHHHHHHHHHHHHHHHHHHCCCHHEEHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEC
NNQWAISTFQAIAGGEGATFAGRGVGCGIASLRVDGNDFLAVYAASRWAAERARRNLGPT
CCCHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCCCHH
LIEWVTYRAGPHSTSDDPTKYRPGDDWTNFPLGDPLERLKRHMIGIGVWSEQDHEDTKAA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
LEAEVLAAQKEAERYGTLADEHVPNVASIFEDVYKEMPAHLRRQRQQLGV
HHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]