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The map label for this gene is rpoN

Identifier: 53723235

GI number: 53723235

Start: 2991302

End: 2992777

Strand: Reverse

Name: rpoN

Synonym: BPSS2218

Alternate gene names: 53723235

Gene position: 2992777-2991302 (Counterclockwise)

Preceding gene: 53723238

Following gene: 53723234

Centisome position: 94.32

GC content: 70.19

Gene sequence:

>1476_bases
ATGTCCGCCACGCTCGCGTTGCAAATGCGTCAACACCTGGCGCTCACGCCGCGCTTGCAGCAGTCGCTGCGTTTGCTCCA
GCTTTCGTCGCTCGAGTTTCAACAGGAACTGCGTCAGGCGCTCGATACCAATCCGTTTCTCGAAGACGTGCAATCGCCCG
ACGACGATGCTGCCGAAGCCGGGCCGAAGCCGGGCGAGACGCCCGCCGCCGACGCAAACGCGAGGGCGGACGACGGCTAC
GCCGAGCGCGACGAGGGCCCGTTCGCGACCGACGCGTCGCCGCCCGCCGGCCAGGACGTGCCGCTGACGGCGGAGCTCTC
GGCGCGCGGCTCGAGCCGGCGCTCCGACGACGCGTCGGATCTCGAGCCCGGCGACTGGATGACGGCCGAGCCGACGCTGC
ACGAGCATCTGCACGACGCGCTGCGCCTTTGCCAGCTCACCCGGCGCGACCGCACGCTCGCGCGCATGATCATCGACGCG
CTCGACGACGACGGCTATCTGCGCCAGGCGCTGCCCGAGCTCGCGGCGGCGGCCGATCCGCTGCTGCATCCGGCCGAGCA
GGAACTGCTCGTCGCGCTGCGGCTCGTGCAGTCGCTCGATCAGCCCGGCATCGGCGCGCGCACGCTGTCCGAATGCCTGT
TGCTGCAGCTCGACGCGATGCCGGCGGACACGCCGGGCGTCGAATGCGCGAAGGAGATCGCCGCGCATCACCTCGAGCGT
CTCGCACGCCGCGAGACGGCCGAGATGCAGCGCCGCATCGGCTGCGACACGCACACGCTGCGCATCGCATGCGCGCTCGT
GCGCCGGCTCGATCCGCGCCCCGGCAACCAGTACGGCAGCACGGCGGGCAACTATGTCGTCCCCGACGTGATCGTGCGGC
AGGTGCGCAACGACTGGCTCGTCACGATCAACCCCGCCGTGATGCCGCGCGCGCGCATCCATCGCCGCTACGCGGAGCTG
TTCGCGCAATCGAGCGGCTCGAATCAGTCGCCGCTCGGCCAGCAACTGCAGGAGGCGCGCTGGCTGATCCGCAACGCGCA
GAAGCGTTTCGACACGATCCGCCGCGTCGGCGAGTGCATCGTCGAGCGGCAGCGCGACTTTTTCCGCTACGGCGAGATCG
CGCTGAAGCCGCTCGTGCTGCGCGACATCGCCGACGAGCTCGGCCTGCACGAATCGACGATCTCGCGCGCGACCGGCAAC
AAGTACATGTCGACGCCGCACGGCACGTTCGAGTTCAAGCACTTCTTCCCGCGCAAGCTCGAGGCGGCGGGCAAGGGCGC
GTGCTCGGCGGCCGTCGCGAGGGTGCTGATCCGCGACATGATCGCGGCCGAACAGGCGATCGATCCGCTGTCGGACGTCG
CGCTCGCGCAGCGTCTGGCGGGGCGCGGGATCGTGCTCGCGCGCCGCACCGTCACGAAGTATCGGCAGGCGATGAAGATC
CCGCCCGCGGAATTGCGCCGCCGCGCGCCTCTATGA

Upstream 100 bases:

>100_bases
ATGCCTATGACGAAAGCCCCGTTCGCACCGTGCGTCGCCAAATTGGTATGGAAGTTGCGGAGGTCCGGGCACCACCGTCC
ATCCAAAAAGGGAGTTCCGC

Downstream 100 bases:

>100_bases
ACCTTCGGCGAGCGCGCGCCGCCGGCGCGCTCGCCGACGACTCGCGAACGCGAAAGGAGCCACCGGCATGCCAGCGAAAT
CGCAAGCCCAGCAGCGCGCC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSATLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQSPDDDAAEAGPKPGETPAADANARADDGY
AERDEGPFATDASPPAGQDVPLTAELSARGSSRRSDDASDLEPGDWMTAEPTLHEHLHDALRLCQLTRRDRTLARMIIDA
LDDDGYLRQALPELAAAADPLLHPAEQELLVALRLVQSLDQPGIGARTLSECLLLQLDAMPADTPGVECAKEIAAHHLER
LARRETAEMQRRIGCDTHTLRIACALVRRLDPRPGNQYGSTAGNYVVPDVIVRQVRNDWLVTINPAVMPRARIHRRYAEL
FAQSSGSNQSPLGQQLQEARWLIRNAQKRFDTIRRVGECIVERQRDFFRYGEIALKPLVLRDIADELGLHESTISRATGN
KYMSTPHGTFEFKHFFPRKLEAAGKGACSAAVARVLIRDMIAAEQAIDPLSDVALAQRLAGRGIVLARRTVTKYRQAMKI
PPAELRRRAPL

Sequences:

>Translated_491_residues
MSATLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQSPDDDAAEAGPKPGETPAADANARADDGY
AERDEGPFATDASPPAGQDVPLTAELSARGSSRRSDDASDLEPGDWMTAEPTLHEHLHDALRLCQLTRRDRTLARMIIDA
LDDDGYLRQALPELAAAADPLLHPAEQELLVALRLVQSLDQPGIGARTLSECLLLQLDAMPADTPGVECAKEIAAHHLER
LARRETAEMQRRIGCDTHTLRIACALVRRLDPRPGNQYGSTAGNYVVPDVIVRQVRNDWLVTINPAVMPRARIHRRYAEL
FAQSSGSNQSPLGQQLQEARWLIRNAQKRFDTIRRVGECIVERQRDFFRYGEIALKPLVLRDIADELGLHESTISRATGN
KYMSTPHGTFEFKHFFPRKLEAAGKGACSAAVARVLIRDMIAAEQAIDPLSDVALAQRLAGRGIVLARRTVTKYRQAMKI
PPAELRRRAPL
>Mature_490_residues
SATLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQSPDDDAAEAGPKPGETPAADANARADDGYA
ERDEGPFATDASPPAGQDVPLTAELSARGSSRRSDDASDLEPGDWMTAEPTLHEHLHDALRLCQLTRRDRTLARMIIDAL
DDDGYLRQALPELAAAADPLLHPAEQELLVALRLVQSLDQPGIGARTLSECLLLQLDAMPADTPGVECAKEIAAHHLERL
ARRETAEMQRRIGCDTHTLRIACALVRRLDPRPGNQYGSTAGNYVVPDVIVRQVRNDWLVTINPAVMPRARIHRRYAELF
AQSSGSNQSPLGQQLQEARWLIRNAQKRFDTIRRVGECIVERQRDFFRYGEIALKPLVLRDIADELGLHESTISRATGNK
YMSTPHGTFEFKHFFPRKLEAAGKGACSAAVARVLIRDMIAAEQAIDPLSDVALAQRLAGRGIVLARRTVTKYRQAMKIP
PAELRRRAPL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=493, Percent_Identity=37.7281947261663, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54492; Mature: 54360

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQSPDDDAAEA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHC
GPKPGETPAADANARADDGYAERDEGPFATDASPPAGQDVPLTAELSARGSSRRSDDASD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCCCCC
LEPGDWMTAEPTLHEHLHDALRLCQLTRRDRTLARMIIDALDDDGYLRQALPELAAAADP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCH
LLHPAEQELLVALRLVQSLDQPGIGARTLSECLLLQLDAMPADTPGVECAKEIAAHHLER
HCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LARRETAEMQRRIGCDTHTLRIACALVRRLDPRPGNQYGSTAGNYVVPDVIVRQVRNDWL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEE
VTINPAVMPRARIHRRYAELFAQSSGSNQSPLGQQLQEARWLIRNAQKRFDTIRRVGECI
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VERQRDFFRYGEIALKPLVLRDIADELGLHESTISRATGNKYMSTPHGTFEFKHFFPRKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
EAAGKGACSAAVARVLIRDMIAAEQAIDPLSDVALAQRLAGRGIVLARRTVTKYRQAMKI
HHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCC
PPAELRRRAPL
CHHHHHHCCCC
>Mature Secondary Structure 
SATLALQMRQHLALTPRLQQSLRLLQLSSLEFQQELRQALDTNPFLEDVQSPDDDAAEA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHC
GPKPGETPAADANARADDGYAERDEGPFATDASPPAGQDVPLTAELSARGSSRRSDDASD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCCCCC
LEPGDWMTAEPTLHEHLHDALRLCQLTRRDRTLARMIIDALDDDGYLRQALPELAAAADP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCH
LLHPAEQELLVALRLVQSLDQPGIGARTLSECLLLQLDAMPADTPGVECAKEIAAHHLER
HCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LARRETAEMQRRIGCDTHTLRIACALVRRLDPRPGNQYGSTAGNYVVPDVIVRQVRNDWL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEE
VTINPAVMPRARIHRRYAELFAQSSGSNQSPLGQQLQEARWLIRNAQKRFDTIRRVGECI
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VERQRDFFRYGEIALKPLVLRDIADELGLHESTISRATGNKYMSTPHGTFEFKHFFPRKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
EAAGKGACSAAVARVLIRDMIAAEQAIDPLSDVALAQRLAGRGIVLARRTVTKYRQAMKI
HHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCC
PPAELRRRAPL
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]