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The map label for this gene is mcl2 [H]

Identifier: 53723166

GI number: 53723166

Start: 2909986

End: 2910858

Strand: Reverse

Name: mcl2 [H]

Synonym: BPSS2150

Alternate gene names: 53723166

Gene position: 2910858-2909986 (Counterclockwise)

Preceding gene: 53723167

Following gene: 53723165

Centisome position: 91.74

GC content: 70.22

Gene sequence:

>873_bases
ATGACGAGCAGCACGAATTACCGCAGTTACCTGTACGTGCCGGCGCACAAGGCAGCCGTCGTCGAGAAGGCCTATGCGAG
CGACGCCGATGCGATCGTGCTCGACCTCGAGGACGCGGTGCCGGCCACTCACAAGGCAGCCGCGCGCGAGGCCGCGGCCG
CGATTCTCGCGGCGGGGCCGGCGAAGCCGACCTACGTGCGGGTCAATCCGTATTCGAGCCCGTGGTGCCGCGACGACGTC
GCGGCGATCGCGCTGCCCGCGCTCAAGGCGCTGCGCCTGCCGAAGTGCGACGCGCCGCAGGAGATCCGCGAAGTGGCCGC
ATGGCTCGACGCGCTCGGCAGCGCGGCCGGCATCCAGATCCTGATCGAATCCGCGTACGGCGTCGAGACCGCGTATCAAC
TCGCGACCGCTTCGCCGCGACTCGAGCGGATCGGGCTCGGCGAGAGCGATCTGCGCGCGGATCTGCAGATCGGCGCCGAC
AATTTCACGCTCGAGGTGTGCCGCGCGCGTTGCGTCGTCGCCGCGCGCGCGGCGGGGCTCGCCGGCCCGATCCAGACCGT
CTATCACACGCTGACCGATCTCGACGGGCTGCGCGAATCGACGCTGCGCGCGAAATCGATGGGCTTTGTCGGCCGCTTCG
CGATCCATCCGTCGCAGGTGCCGGTGATCAACGAGGTATTCACGCCGTCCGCCGACGAGATCGAGGCGGCCGAGCGGATT
CTCGATGCGCTCGACGCGGCCGCCGGCGCGTCGAGCGCGTCGTCGGTGTTCGTGCTGCCCGACGGGCGCGTCGTCGCGCC
GCCGCTGATCGCGAACGCGCGCGTCACGCTCGCGCTCGCGGGCAACCTGCGTTCGAACGGAGTCCTGTCATGA

Upstream 100 bases:

>100_bases
GCTGGGGCGGGCCGCGGCGCGGCGCGCACAACGACGAAGTGTTCGGCGCGCTCGGCATCGATGCCGACGAACTCGCCGAT
TTGACCGAACGAGGCGCACT

Downstream 100 bases:

>100_bases
GCGAAGCCGATCTGACCATCGTACGGGCCGCCGCGCGCCGCGCCGCGCCCGTCGCCACGCCGCCCGCGGACGGCATCGTC
GGCGCGCTCGGACGCTTCGC

Product: citrate lyase

Products: Acetate; Oxaloacetate [C]

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTSSTNYRSYLYVPAHKAAVVEKAYASDADAIVLDLEDAVPATHKAAAREAAAAILAAGPAKPTYVRVNPYSSPWCRDDV
AAIALPALKALRLPKCDAPQEIREVAAWLDALGSAAGIQILIESAYGVETAYQLATASPRLERIGLGESDLRADLQIGAD
NFTLEVCRARCVVAARAAGLAGPIQTVYHTLTDLDGLRESTLRAKSMGFVGRFAIHPSQVPVINEVFTPSADEIEAAERI
LDALDAAAGASSASSVFVLPDGRVVAPPLIANARVTLALAGNLRSNGVLS

Sequences:

>Translated_290_residues
MTSSTNYRSYLYVPAHKAAVVEKAYASDADAIVLDLEDAVPATHKAAAREAAAAILAAGPAKPTYVRVNPYSSPWCRDDV
AAIALPALKALRLPKCDAPQEIREVAAWLDALGSAAGIQILIESAYGVETAYQLATASPRLERIGLGESDLRADLQIGAD
NFTLEVCRARCVVAARAAGLAGPIQTVYHTLTDLDGLRESTLRAKSMGFVGRFAIHPSQVPVINEVFTPSADEIEAAERI
LDALDAAAGASSASSVFVLPDGRVVAPPLIANARVTLALAGNLRSNGVLS
>Mature_289_residues
TSSTNYRSYLYVPAHKAAVVEKAYASDADAIVLDLEDAVPATHKAAAREAAAAILAAGPAKPTYVRVNPYSSPWCRDDVA
AIALPALKALRLPKCDAPQEIREVAAWLDALGSAAGIQILIESAYGVETAYQLATASPRLERIGLGESDLRADLQIGADN
FTLEVCRARCVVAARAAGLAGPIQTVYHTLTDLDGLRESTLRAKSMGFVGRFAIHPSQVPVINEVFTPSADEIEAAERIL
DALDAAAGASSASSVFVLPDGRVVAPPLIANARVTLALAGNLRSNGVLS

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=301, Percent_Identity=29.2358803986711, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI87081764, Length=284, Percent_Identity=32.0422535211268, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557458, Length=305, Percent_Identity=29.5081967213115, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6 [C]

Molecular weight: Translated: 30358; Mature: 30227

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSTNYRSYLYVPAHKAAVVEKAYASDADAIVLDLEDAVPATHKAAAREAAAAILAAGP
CCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
AKPTYVRVNPYSSPWCRDDVAAIALPALKALRLPKCDAPQEIREVAAWLDALGSAAGIQI
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
LIESAYGVETAYQLATASPRLERIGLGESDLRADLQIGADNFTLEVCRARCVVAARAAGL
EEECCCCHHHHHHHHCCCCCHHHCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
AGPIQTVYHTLTDLDGLRESTLRAKSMGFVGRFAIHPSQVPVINEVFTPSADEIEAAERI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCHHHHHHHHHH
LDALDAAAGASSASSVFVLPDGRVVAPPLIANARVTLALAGNLRSNGVLS
HHHHHHHCCCCCCCEEEEECCCCEECCCEECCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TSSTNYRSYLYVPAHKAAVVEKAYASDADAIVLDLEDAVPATHKAAAREAAAAILAAGP
CCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
AKPTYVRVNPYSSPWCRDDVAAIALPALKALRLPKCDAPQEIREVAAWLDALGSAAGIQI
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
LIESAYGVETAYQLATASPRLERIGLGESDLRADLQIGADNFTLEVCRARCVVAARAAGL
EEECCCCHHHHHHHHCCCCCHHHCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
AGPIQTVYHTLTDLDGLRESTLRAKSMGFVGRFAIHPSQVPVINEVFTPSADEIEAAERI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCHHHHHHHHHH
LDALDAAAGASSASSVFVLPDGRVVAPPLIANARVTLALAGNLRSNGVLS
HHHHHHHCCCCCCCEEEEECCCCEECCCEECCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: Citrate [C]

Specific reaction: Citrate --> Acetate + Oxaloacetate [C]

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA