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The map label for this gene is lutR [H]

Identifier: 53723091

GI number: 53723091

Start: 2808477

End: 2809190

Strand: Reverse

Name: lutR [H]

Synonym: BPSS2073

Alternate gene names: 53723091

Gene position: 2809190-2808477 (Counterclockwise)

Preceding gene: 53723093

Following gene: 53723090

Centisome position: 88.53

GC content: 61.62

Gene sequence:

>714_bases
ATGGACGCAGACTTCTCGAATGACATCCCCTCGACCTTGACTTCCAGTGTCACGGACGCGGTGATCAATCTTATTCATGA
AACGCGACTCGTCCCCAACGATAGCCTTCCCAGCGAAGGAGAGTTCGCCCAGAAGCTGGGTGTGGGACGTGGCGTCGTAC
GTGAAGCCTTCAAGGCTCTCGAAGCACTTGGCATCTTGGAGCTTGCGCCCGGCAAGCGGGCTCGCGTTGGCAAGCTCAAG
AGTCACGTGCTCGCCATGCTCATGGACCACGCGGTGGTCACGATGCAGGTCAGCGTCCAGCAGACGCTTGATTTGCGCCG
CACATTGGAAGTCCGGACGGCCCGGCTCGCGGCGCTTCGCCGGAGCGAAACCGAACTTGCGGCCATTCGGGAGGCGGCCG
CAGCCATGCGCCAGCACGGCAATGGCCGCGAGTCGCCGACCGAAGAGGACATTGCCTTTCACGTCGCCATCGCTCGCGCC
GCACAGAACCCCCTGTATGCATTGCTCATTGAGGGCTTCCGTCTCGTGATCCGGCAGACCGCCCCGATCAGTTGGGCGGT
GCGACAAAGCCATGAGGAACGCACGGCTGTGCATGACATGCACGACGCAATCGTCGACGCGATTGCCCGACAAGATGCCG
CCGCTGCCGAAGTGGCCATGGCGGCCCATTTTGACAACAGTGTCCAGGCTTTGATTTCTGCCGGCGTGACCTAA

Upstream 100 bases:

>100_bases
TATTGAAAACCTGTACGATAGGTTTAGCATTCTCGTCATGCAAGCTGACCTCGTCAAGTGCGACAATCGTCTCTTTCAAA
TCAGAGCTGAAGGAGCCGAT

Downstream 100 bases:

>100_bases
AGGAGCAAGTTTGAAAATCACCGAAATCGAAACCCTGCGCCCCGAGGAATTTCCGAACCTGTTATGGGTGCTGGTTCATA
CCGACGAAGGCATTACTGGC

Product: GntR family regulator protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MDADFSNDIPSTLTSSVTDAVINLIHETRLVPNDSLPSEGEFAQKLGVGRGVVREAFKALEALGILELAPGKRARVGKLK
SHVLAMLMDHAVVTMQVSVQQTLDLRRTLEVRTARLAALRRSETELAAIREAAAAMRQHGNGRESPTEEDIAFHVAIARA
AQNPLYALLIEGFRLVIRQTAPISWAVRQSHEERTAVHDMHDAIVDAIARQDAAAAEVAMAAHFDNSVQALISAGVT

Sequences:

>Translated_237_residues
MDADFSNDIPSTLTSSVTDAVINLIHETRLVPNDSLPSEGEFAQKLGVGRGVVREAFKALEALGILELAPGKRARVGKLK
SHVLAMLMDHAVVTMQVSVQQTLDLRRTLEVRTARLAALRRSETELAAIREAAAAMRQHGNGRESPTEEDIAFHVAIARA
AQNPLYALLIEGFRLVIRQTAPISWAVRQSHEERTAVHDMHDAIVDAIARQDAAAAEVAMAAHFDNSVQALISAGVT
>Mature_237_residues
MDADFSNDIPSTLTSSVTDAVINLIHETRLVPNDSLPSEGEFAQKLGVGRGVVREAFKALEALGILELAPGKRARVGKLK
SHVLAMLMDHAVVTMQVSVQQTLDLRRTLEVRTARLAALRRSETELAAIREAAAAMRQHGNGRESPTEEDIAFHVAIARA
AQNPLYALLIEGFRLVIRQTAPISWAVRQSHEERTAVHDMHDAIVDAIARQDAAAAEVAMAAHFDNSVQALISAGVT

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994955, Length=218, Percent_Identity=26.605504587156, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790780, Length=218, Percent_Identity=31.651376146789, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1789621, Length=229, Percent_Identity=26.6375545851528, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082208, Length=200, Percent_Identity=28, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1789353, Length=162, Percent_Identity=29.6296296296296, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25665; Mature: 25665

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDADFSNDIPSTLTSSVTDAVINLIHETRLVPNDSLPSEGEFAQKLGVGRGVVREAFKAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
EALGILELAPGKRARVGKLKSHVLAMLMDHAVVTMQVSVQQTLDLRRTLEVRTARLAALR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSETELAAIREAAAAMRQHGNGRESPTEEDIAFHVAIARAAQNPLYALLIEGFRLVIRQT
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
APISWAVRQSHEERTAVHDMHDAIVDAIARQDAAAAEVAMAAHFDNSVQALISAGVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MDADFSNDIPSTLTSSVTDAVINLIHETRLVPNDSLPSEGEFAQKLGVGRGVVREAFKAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
EALGILELAPGKRARVGKLKSHVLAMLMDHAVVTMQVSVQQTLDLRRTLEVRTARLAALR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSETELAAIREAAAAMRQHGNGRESPTEEDIAFHVAIARAAQNPLYALLIEGFRLVIRQT
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
APISWAVRQSHEERTAVHDMHDAIVDAIARQDAAAAEVAMAAHFDNSVQALISAGVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]