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The map label for this gene is xylX [H]

Identifier: 53722909

GI number: 53722909

Start: 2563271

End: 2564527

Strand: Reverse

Name: xylX [H]

Synonym: BPSS1888

Alternate gene names: 53722909

Gene position: 2564527-2563271 (Counterclockwise)

Preceding gene: 53722911

Following gene: 53722908

Centisome position: 80.82

GC content: 64.44

Gene sequence:

>1257_bases
GTGGCGCTGATCGATCGCGCCGATTCGTCCGACGTGCGCTTTCCGCGCGACGACGGCTCGCGCGTGCCGTACAAGGTGTT
CAGCTCGCAGGCGGTGTACGAGCGCGAGCAGGAGCGGATCTTCCGCGGGCCGACGTGGAATTTCGTCGCGCTCGAAGCGG
AAATTCCGAAGCCGGGCGATTTCAAGAGCACGTTCGTCGGCGACACGCCCGTCGTCGTCACGCGCGGCGAGGACGGCGCG
CTCGCCGCGTGGGTGAACCGCTGCGCGCATCGCGGCGCGCAGGTGTGCCGCAAGGCGCGCGGCAACGCGAGCTCGCACAC
GTGCGTGTATCACCAGTGGAGCTTCGACAGCCGGGGCGACCTGCTCGGCGTGCCGTTCCGGCGCGGCCAGAAGGGCATGG
CCGGGATGCCGGCCGATTTCGATCCGAAGAACCACGGGCTGCGCAAGCTGCGCGTCGACAGCTACAAGGGCCTCGTGTTC
GCGACGTTCAGCGACGAGGTCGCGCCGCTGCCCGATTACCTCGGCGCGCAGATGCGGCCGTGGATCGACCGGATCTTCCA
CAAGCCGATCGAGTATCTCGGCTGCACGCGGCAGTTCTCGAAATCGAACTGGAAGCTGTACTTCGAGAACGTGAAGGACC
CGTATCACGCGAGCATGCTGCATCTGTTCCATACGACGTTCAATATTTTCCGCGTCGGCATGAAGGCGCGCTCGATTCCC
GATGCGACGCACGGGCTGCACAGCATCATCACGATGACGAAGACGCAAGACGACGCGGACACCGCGAGCGCGTACAAGCA
GCAGAACATCCGTTCGTTCGACGAAGGTTTCTCGCTCGAGGACGATTCGATCCTCGGCCTCGTGTCCGAGTACGACGAGG
ACACGACGAACCACATCCAGCCGATCTTCCCGCAGCTCGTGATCCAGCAGATCCACAACACGCTCGTCGCACGCCAGTTG
CTGCCGAAGGGGCCGAAGAACTTCGAATTGATCTTCCACTTCTTCGGCTATGCGGACGACACGCCCGAGCTGCGCGATTT
GCGGATCAAGCAGGCGAACCTCGTCGGGCCCGCGGGCTACATCTCGATGGAGGACACCGAGGCGACCGAGCTCGTGCAGC
GCGGCACCGTGCGCGACGCAAACGCGGCGTCGGTGATCGAGATGTCGCGCGGCAATCCGGACCAGCAGGACACGGCGATC
ACCGAGAGCCTGATCCGCAGGTTCTGGGTCGGCTACCAGCAGTTGATGGGCTATTGA

Upstream 100 bases:

>100_bases
GCGGATTGTCCAGCGCCTCAACCGCAGCAACTTGCATGGGATCGGAGCGGGCGCCCGCTCCGACCGACAGGAGACAATGA
TGGAAATGACCGAATCGCCG

Downstream 100 bases:

>100_bases
AGCGGGAGACGAACATGACCGACATCACCGACGGCCTCACCGGCGACATTCGCACGTGGTTCGAGCTGCACATGCTGCAG
AACCGCTACATCGGGCATCT

Product: aromatic oxygenase

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGDFKSTFVGDTPVVVTRGEDGA
LAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGDLLGVPFRRGQKGMAGMPADFDPKNHGLRKLRVDSYKGLVF
ATFSDEVAPLPDYLGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVGMKARSIP
DATHGLHSIITMTKTQDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQPIFPQLVIQQIHNTLVARQL
LPKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNPDQQDTAI
TESLIRRFWVGYQQLMGY

Sequences:

>Translated_418_residues
MALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGDFKSTFVGDTPVVVTRGEDGA
LAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGDLLGVPFRRGQKGMAGMPADFDPKNHGLRKLRVDSYKGLVF
ATFSDEVAPLPDYLGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVGMKARSIP
DATHGLHSIITMTKTQDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQPIFPQLVIQQIHNTLVARQL
LPKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNPDQQDTAI
TESLIRRFWVGYQQLMGY
>Mature_417_residues
ALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGDFKSTFVGDTPVVVTRGEDGAL
AAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGDLLGVPFRRGQKGMAGMPADFDPKNHGLRKLRVDSYKGLVFA
TFSDEVAPLPDYLGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVGMKARSIPD
ATHGLHSIITMTKTQDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQPIFPQLVIQQIHNTLVARQLL
PKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNPDQQDTAIT
ESLIRRFWVGYQQLMGY

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=408, Percent_Identity=27.2058823529412, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788103, Length=203, Percent_Identity=26.6009852216749, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 47428; Mature: 47297

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGD
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
FKSTFVGDTPVVVTRGEDGALAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGD
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
LLGVPFRRGQKGMAGMPADFDPKNHGLRKLRVDSYKGLVFATFSDEVAPLPDYLGAQMRP
EECCCHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHCCCCCH
WIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVGMKARSIP
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DATHGLHSIITMTKTQDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQ
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHH
PIFPQLVIQQIHNTLVARQLLPKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHEEECCCCCCCCHHHHHEEHHHCCCCCCCE
ISMEDTEATELVQRGTVRDANAASVIEMSRGNPDQQDTAITESLIRRFWVGYQQLMGY
EECCCCHHHHHHHHCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGD
CCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
FKSTFVGDTPVVVTRGEDGALAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGD
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
LLGVPFRRGQKGMAGMPADFDPKNHGLRKLRVDSYKGLVFATFSDEVAPLPDYLGAQMRP
EECCCHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHCCCCCH
WIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVGMKARSIP
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DATHGLHSIITMTKTQDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQ
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHH
PIFPQLVIQQIHNTLVARQLLPKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHEEECCCCCCCCHHHHHEEHHHCCCCCCCE
ISMEDTEATELVQRGTVRDANAASVIEMSRGNPDQQDTAITESLIRRFWVGYQQLMGY
EECCCCHHHHHHHHCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]