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The map label for this gene is thcB [H]

Identifier: 53722675

GI number: 53722675

Start: 2272681

End: 2275035

Strand: Reverse

Name: thcB [H]

Synonym: BPSS1654

Alternate gene names: 53722675

Gene position: 2275035-2272681 (Counterclockwise)

Preceding gene: 53722683

Following gene: 53722674

Centisome position: 71.7

GC content: 70.15

Gene sequence:

>2355_bases
ATGACGACGCATTCCGCGTCCACGCCGCCGGCCTTGCCCGCCGCGCAGTGTCCGTTCCGCCCCGTTTCAGACGATGCCGC
CGCGCATCCGGCCCGTTGCCCGGCGAGCGCCGGGGCGGCGGCATTCGATCCGTTCGACGACGCCTATCAACAGGACCCGC
CCGAGTACGTGCGTTGGGCGCGCGAGCGCGAGCCGGTGTTCTACAGCCCGAAGCTCGGTTACTGGGTCGTCGCGCGCTAT
GACGACATCAAGACGATCTTTCGCGACAACCGGACCTTCAGCCCGTCGATCGCGCTCGAGAAGATCACGCCGACGGGGCC
GGACGCGAACGCGGCGCTCGCGTCGTACGGCTATGCGATGGGCCGCACGCTCGTCAACGAGGACGAGCCCACGCACATGG
CGCGGCGGCGTCTGCTGATCGAGCCGTTCACGCCCGACGCGCTGCGCGCGCATGAGCCGATGGTGCGACGGCTTGCGCGC
GAATACGTCGACCGCTTCGTCGACGATGGCCGCGCGGACCTCGTCGACCAGATGCTGTGGGAAGTGCCGCTCACGGTCGC
GCTGCATTTTCTGGGCGTGCCCGAAGCGGACATGGATACGCTGCGCGCGTACTCGATCGCGCATACCGTCAACACCTGGG
GACGGCCGCGGCCGGACGAGCAAGTTGCGGTCGCGCATGCGGTCGGACAGTTCTGGCAGTACGCGGGCAAGGTGCTCGGG
AAGATGCGGGAGGATCCGTCGGGGCCCGGCTGGATGCAGTTCGGCATCCGCATGCAGCGCGAGCATCCGGACATCGTCAC
CGATTCGTATCTGCATTCGATGATGATGGCGGGCATCGTTGCCGCGCACGAGACCACCGCGAACGCAACGGCGAACGCGC
TGAAGCTGTTGCTCGAGCATCCGCGCGCGTGGCGAGAGCTGTGTGACGACCCTGCGCTGATCCCGAACGCGGTCGACGAG
TGCCTGCGTCACAGCGGATCGATCGTCGCGTGGCGGCGGATCGCGACGCGCGACGCGACGGTCGGCGGCGTTGCGATTCC
CGCCGGCGCGAAGCTGTTGATCGTCACGTCGTCGGCGAATCGTGACGAGCGCCGCTTCGCCGATCCGGACCTGTTCGACA
TCCACCGCGACAATCCCGCCGATCACCTGACGTTCGGCTACGGTTCGCATCAGTGCATGGGCAAGCATCTCGCGCGCATG
GAGATGCAGATCTTCATCGACGAGCTGTCGCGCCGCCTGCCGCACATGCGGCTCGCCGAACAGCGCTTCACGTATGTGCC
GAACACGTCGTTTCGTGGTCCGGAGCATCTGTGGGTCGAATGGGCGCCGGCGGAGAATCCGCAGCGGCGCGGCCACGGCG
CGCGCCGCTGCGCGCTTGCCGTGCGCATCGGCGAATCGAACGCGCGCCGCGCGGGGCGCGCGCTGCGCGTCGACAGCGTG
ATGCCGGTGGCCGAGCGCGTGCTGCGCGTACGCCTCGTCGCGCCCGACGGCGCGCCGCTGCCGCGCTGGAGCGCGGGCGC
GCATATCGACGTTGAATGCGGCGACACCGGGCTCTCCCGACAGTACTCGCTGTGCGGCGATCCGGCCGAGCGCGGCGTAT
ACGAGATCGCGGTGCTGCGCGAGCCGGCGAGCCGCGGCGGCTCCGCATGGCTGCACGAGCGGCTGCGGCCCGGCATGCGC
GTAAAGATTCGCGGGCCGCGCAACCACTTCCGGCTCGACGGGCACGCCGCCCGCGCGATCCTGATCGCGGGCGGCATCGG
CGTCACGCCGATCGGCGCGATCGCACGCGAGGCGCGCGCGCGCGGCATCGACTACGCGCTGCATTACTGCGGCCGCAGCC
GCCGGACGATGGCGTTCGTCGACGAGCTCGCCGCGCTGCACGGCGAGCGGCTTCGCCTGCACGTGTCGGACGAAGGCACG
CGGCATGATTTTTCCGCGCTCGACGTCGCTGCCGGCACGCAGGTGTATGCGTGCGGTCCCGCGCGCATGCTCGACGCGCT
GGCCGCGTGCGCCGCGCATTGGCCGGCGCATGCGCTGCGCGTCGAGCATTTCGCGGCCGCCGCGCCGCGCGTCGATCCGC
GCACCGATCAGCCGTTCGAGGTCGAGCTGAAGGATTCGGGGCTCACGCTGACGGTGCCCGCGAACCGGACGCTGCTGGCG
ACGCTGCGCGCGGCGAACGTCGACGTGCAGAGCGACTGCGAGGAAGGGCTGTGCGGCTCGTGCGAGGTGCGCGTGCTTGC
CGGCGAGGTTGACCATCGTGACAGCGTGCTGAGCGCGGACGAGCGTGCGCTGAACACGCGCATGATGGCGTGCTGCTCGC
GCGCGAGGCGCGGACGGCTCGTGCTCGAACTGTAG

Upstream 100 bases:

>100_bases
GGCGATGCAGGTTAACGCGATCATTGATTTTGCGCAGTGCGTACTAGCGTTACGCAAAGCGCAATTCGATCCAGCGTAAT
TCGACCCCTGGAGGACTGCC

Downstream 100 bases:

>100_bases
CTGTAGCGTGAGGCTCGTCGCCCCGGCGCGGGACGGACGAAGACAACGGCATGGCGGGAGGCGACGTATGACGGCGCGAG
TGGACGTGCGCGCGAGGCTC

Product: cytochrome P450

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 784; Mature: 783

Protein sequence:

>784_residues
MTTHSASTPPALPAAQCPFRPVSDDAAAHPARCPASAGAAAFDPFDDAYQQDPPEYVRWAREREPVFYSPKLGYWVVARY
DDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAMGRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAR
EYVDRFVDDGRADLVDQMLWEVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLG
KMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEHPRAWRELCDDPALIPNAVDE
CLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSANRDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARM
EMQIFIDELSRRLPHMRLAEQRFTYVPNTSFRGPEHLWVEWAPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLREPASRGGSAWLHERLRPGMR
VKIRGPRNHFRLDGHAARAILIAGGIGVTPIGAIAREARARGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGT
RHDFSALDVAAGTQVYACGPARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARRGRLVLEL

Sequences:

>Translated_784_residues
MTTHSASTPPALPAAQCPFRPVSDDAAAHPARCPASAGAAAFDPFDDAYQQDPPEYVRWAREREPVFYSPKLGYWVVARY
DDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAMGRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAR
EYVDRFVDDGRADLVDQMLWEVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLG
KMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEHPRAWRELCDDPALIPNAVDE
CLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSANRDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARM
EMQIFIDELSRRLPHMRLAEQRFTYVPNTSFRGPEHLWVEWAPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLREPASRGGSAWLHERLRPGMR
VKIRGPRNHFRLDGHAARAILIAGGIGVTPIGAIAREARARGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGT
RHDFSALDVAAGTQVYACGPARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARRGRLVLEL
>Mature_783_residues
TTHSASTPPALPAAQCPFRPVSDDAAAHPARCPASAGAAAFDPFDDAYQQDPPEYVRWAREREPVFYSPKLGYWVVARYD
DIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAMGRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLARE
YVDRFVDDGRADLVDQMLWEVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLGK
MREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEHPRAWRELCDDPALIPNAVDEC
LRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSANRDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARME
MQIFIDELSRRLPHMRLAEQRFTYVPNTSFRGPEHLWVEWAPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSVM
PVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLREPASRGGSAWLHERLRPGMRV
KIRGPRNHFRLDGHAARAILIAGGIGVTPIGAIAREARARGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGTR
HDFSALDVAAGTQVYACGPARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLAT
LRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARRGRLVLEL

Specific function: Degradation of thiocarbamate herbicides [H]

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=320, Percent_Identity=40.3125, Blast_Score=221, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 86771; Mature: 86639

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHSASTPPALPAAQCPFRPVSDDAAAHPARCPASAGAAAFDPFDDAYQQDPPEYVRWA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
REREPVFYSPKLGYWVVARYDDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAM
HHCCCEEECCCCCEEEEEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAREYVDRFVDDGRADLVDQMLW
CCHHCCCCCHHHHHHHHHEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEH
HHHCCCCCCCCEEECEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
PRAWRELCDDPALIPNAVDECLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSAN
CHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCCCEEEEEECCCC
RDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARMEMQIFIDELSRRLPHMRLAE
CCHHHCCCCCCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRFTYVPNTSFRGPEHLWVEWAPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
HHHEECCCCCCCCCCEEEEEECCCCCHHHHCCCHHEEEEEEEECCCCHHHCCCEEEECHH
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLR
HHHHHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCEEEEEEEE
EPASRGGSAWLHERLRPGMRVKIRGPRNHFRLDGHAARAILIAGGIGVTPIGAIAREARA
CCCCCCCHHHHHHHCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
RGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGTRHDFSALDVAAGTQVYACGP
CCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEECCH
ARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEE
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARRGRL
EHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLEL
EEEC
>Mature Secondary Structure 
TTHSASTPPALPAAQCPFRPVSDDAAAHPARCPASAGAAAFDPFDDAYQQDPPEYVRWA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
REREPVFYSPKLGYWVVARYDDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAM
HHCCCEEECCCCCEEEEEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAREYVDRFVDDGRADLVDQMLW
CCHHCCCCCHHHHHHHHHEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEH
HHHCCCCCCCCEEECEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
PRAWRELCDDPALIPNAVDECLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSAN
CHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCCCEEEEEECCCC
RDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARMEMQIFIDELSRRLPHMRLAE
CCHHHCCCCCCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRFTYVPNTSFRGPEHLWVEWAPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
HHHEECCCCCCCCCCEEEEEECCCCCHHHHCCCHHEEEEEEEECCCCHHHCCCEEEECHH
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLR
HHHHHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCEEEEEEEE
EPASRGGSAWLHERLRPGMRVKIRGPRNHFRLDGHAARAILIAGGIGVTPIGAIAREARA
CCCCCCCHHHHHHHCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
RGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGTRHDFSALDVAAGTQVYACGP
CCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEECCH
ARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEE
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARRGRL
EHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLEL
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]