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The map label for this gene is poxB

Identifier: 53722659

GI number: 53722659

Start: 2253512

End: 2255230

Strand: Reverse

Name: poxB

Synonym: BPSS1636

Alternate gene names: 53722659

Gene position: 2255230-2253512 (Counterclockwise)

Preceding gene: 53722660

Following gene: 53722658

Centisome position: 71.08

GC content: 68.47

Gene sequence:

>1719_bases
ATGGCGAGCACGATTGCCGACTACCTCGCGCGGACGCTGGCCGCCGCCGGCGTCACGAAGATCTGGGGCGTGACGGGCGA
CAGCCTGAACGGATTGTCCGACAGCCTGCGCCGCGACGGCGGCATTCGCTGGATGCATACCCGGCATGAAGAGGTGGCGG
CGTTCGCCGCGGGCGCCGAAGCGGCGCAGACCGGCCGGCTGGCCGTATGCGCGGGCAGTTGCGGGCCGGGCAATCTGCAT
CTGATCAACGGCTTGTTCGATTGCCATCGCAATCATGTGCCGGTGCTCGCGATCGCCGCGCAGATTCCGTCGACCGAAAT
CGGGCTCGGCTATTTTCAGGAAACGCATCCGCAGGAACTGTTTCGCGAATGCAGCCATTACGTCGAGCTCGTGACGAACG
CATCGCAATTTCCGCGCGTGCTCGAGCGCGCGATGCGCGCCGCGATCGGCGAGCACGGCGTCGCGGTGATCGTGCTCCCG
GGCGATGTCGCGCTGAGCGCGGCGCCCGCGCGGACACCGTCGTGGTTCGATTTCGCCTCGCCGGTGACGACGCCCGTCGA
CGCCGATCTGGAGCGGCTCGCGGCGTTGCTCGACGGCTCCGATGCGGTCACGCTGCTGTGCGGCAGCGGTTGCGCGGGCG
CGCACGACGAAGTCGTCGCGTTGGCCGATGCGCTCGCGGCGCCCGTCGTGCATGCGCTGCGCGGCAAGCAGCACATTGAA
TACGACAATCCGAACAGCGTCGGCATGACGGGGCTGATCGGCTTCAGCTCCGGCTATCACGCGATGCAATCGTGCGACAC
GCTGCTGATGCTCGGCACCGATTTCCCCTATCGCAATTTCTATCCGACGCGCGCCCGGATCGCGCAGATCGACCGCCGCG
CGTCGGCGCTCGGCCGTCGCGCGCCGCTCGACGTCGGCCTCGTCGGCGACGTGCGCGCCACGCTGTCCGCGCTGTTGCCG
CGCATCCGGCGCAAGACGCGGCGCGGTTTCGTCGAGCGTGCGCTTGCGCATTATGCCGCCGCGCGCAAGGGGCTCGACGA
ACTGGCGAGACCGTCGCCGGCCGGGCGGCCGATTCATCCGCAGTACTTGACGCGCGTCGTCGACGAGCTCGCCGCCGCCG
ATGCGATCTTCGCCGCGGACGTCGGCACGCCGACGCTGTGGGCGGCGCGCTATCTGACGATGAACGGCCGCAGGCGGCTG
CACGGATCGTTCAATCACGGCTCGATGGCGAACGCGTTGCCGCAGGCACTGGGCGCGCAGGCCGCGCATCCCGGGCGGCA
AGTCGTGTCGCTGTCGGGCGACGGCGGGCTGTCGATGCTCCTCGGCGATCTGCTGAGCGCGCGGCAACTCGATTTGCCGA
TCAAGGTGATCGTCTACAACAACAGCTCGCTGGGCTTCGTCGCGATGGAGATGAAGGCGGGCGGTTATCTGGATACGGGC
ACCGATCTGCACGAGACCGATTTCGCGGCGATCGCGAAGGGGGCGGGCCTCTACAGCCTGCGCGTGACCGAATCGGAAGC
GTTGCCCGATGCGCTCGGCAAGCTGCTCGCGCACCCGGGGCCCGCGCTGCTCGACGTCGTCACCGACAAGCACGAGCTCG
CGATGCCGCCGAAGATCGAATGGGCGCAGGCGAAGGGATTCAGCCTCTACATGTTGCGCGCGGTGCTGAGCGGGCGCGGC
GACGAAGTGCTCGAGCTCGCGCAGACGAATCTGCGGTGA

Upstream 100 bases:

>100_bases
CGAACGGGCGCGCGCGCTGATCCGGCTCGCGCACCCCGATTTTCGCGACGCGCTGGGCGTGCACGCACGCCGGCTGCACT
TGATCTGAATGGAGGATCGC

Downstream 100 bases:

>100_bases
AGGCACGGGGGTGGCTGCGCGATGCGCCGCCCCATTGAACGCGGGCATCGCGAATGGCTACCGGCGTGCGCCGCGCCTCG
TCGACCCAGCCCGCCCGCGT

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAEAAQTGRLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAQIPSTEIGLGYFQETHPQELFRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLP
GDVALSAAPARTPSWFDFASPVTTPVDADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRRAPLDVGLVGDVRATLSALLP
RIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHPQYLTRVVDELAAADAIFAADVGTPTLWAARYLTMNGRRRL
HGSFNHGSMANALPQALGAQAAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGKLLAHPGPALLDVVTDKHELAMPPKIEWAQAKGFSLYMLRAVLSGRG
DEVLELAQTNLR

Sequences:

>Translated_572_residues
MASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAEAAQTGRLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAQIPSTEIGLGYFQETHPQELFRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLP
GDVALSAAPARTPSWFDFASPVTTPVDADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRRAPLDVGLVGDVRATLSALLP
RIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHPQYLTRVVDELAAADAIFAADVGTPTLWAARYLTMNGRRRL
HGSFNHGSMANALPQALGAQAAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGKLLAHPGPALLDVVTDKHELAMPPKIEWAQAKGFSLYMLRAVLSGRG
DEVLELAQTNLR
>Mature_571_residues
ASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAEAAQTGRLAVCAGSCGPGNLHL
INGLFDCHRNHVPVLAIAAQIPSTEIGLGYFQETHPQELFRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLPG
DVALSAAPARTPSWFDFASPVTTPVDADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIEY
DNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRRAPLDVGLVGDVRATLSALLPR
IRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHPQYLTRVVDELAAADAIFAADVGTPTLWAARYLTMNGRRRLH
GSFNHGSMANALPQALGAQAAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTGT
DLHETDFAAIAKGAGLYSLRVTESEALPDALGKLLAHPGPALLDVVTDKHELAMPPKIEWAQAKGFSLYMLRAVLSGRGD
EVLELAQTNLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=488, Percent_Identity=23.5655737704918, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=64.4483362521891, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=28.1818181818182, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=26.2206148282098, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1786717, Length=550, Percent_Identity=25.0909090909091, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788716, Length=542, Percent_Identity=20.479704797048, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17531299, Length=483, Percent_Identity=24.4306418219462, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=483, Percent_Identity=24.4306418219462, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=24.1992882562278, Blast_Score=156, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=503, Percent_Identity=21.8687872763419, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=494, Percent_Identity=22.6720647773279, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922626, Length=477, Percent_Identity=24.9475890985325, Blast_Score=106, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 60867; Mature: 60736

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHCCH
AAQTGRLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAQIPSTEIGLGYFQETHPQEL
HHCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHH
FRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLPGDVALSAAPARTPSWFDFAS
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCCC
PVTTPVDADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
CCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRR
CCCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
APLDVGLVGDVRATLSALLPRIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCH
QYLTRVVDELAAADAIFAADVGTPTLWAARYLTMNGRRRLHGSFNHGSMANALPQALGAQ
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCC
AAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCC
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGKLLAHPGPALLDVVTDKHELAMPPKIE
CCCCHHHHHHHHCCCCEEEEEECCHHCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCC
WAQAKGFSLYMLRAVLSGRGDEVLELAQTNLR
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAE
CHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHCCH
AAQTGRLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAQIPSTEIGLGYFQETHPQEL
HHCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHH
FRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLPGDVALSAAPARTPSWFDFAS
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCCC
PVTTPVDADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
CCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRR
CCCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
APLDVGLVGDVRATLSALLPRIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCH
QYLTRVVDELAAADAIFAADVGTPTLWAARYLTMNGRRRLHGSFNHGSMANALPQALGAQ
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCC
AAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCC
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGKLLAHPGPALLDVVTDKHELAMPPKIE
CCCCHHHHHHHHCCCCEEEEEECCHHCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCC
WAQAKGFSLYMLRAVLSGRGDEVLELAQTNLR
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]