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The map label for this gene is phoB [H]

Identifier: 53722627

GI number: 53722627

Start: 2180594

End: 2181328

Strand: Reverse

Name: phoB [H]

Synonym: BPSS1604

Alternate gene names: 53722627

Gene position: 2181328-2180594 (Counterclockwise)

Preceding gene: 53722628

Following gene: 53722626

Centisome position: 68.75

GC content: 70.07

Gene sequence:

>735_bases
ATGCAGCGCACCGTGCAGCAGCCCAAGCAGATCGTCGTCGTCGAGGACGATCCGGTGCAGCGCACGCTGCTCGTCACGTG
GCTGAAAGCCGAGGGCTATCGCGTCGAGGCGTTCGACGACGGGCTCGATGCGCGCCGTTTTCTCGGCGACAGCTGGGCCG
ATCTGCTGCTGCTCGACTGGGATCTGCCCGGGATGACCGGCGAGCGGCTGCTCGCGTGGGTGCGCGGCCGCGCGCGCTCG
ATCGTGCCGGTGATTTTCCAGACCGTGCACTCGGACGAAGAGGACATCGTGAAGATTCTCGATGCCGGCGCGGACGATTT
CCTCATCAAGCCGCTCGAAAGGCAGACGCTGCTCGCGCGCGTGCGCGCGCTGCTGCGCCGCTTCGCGGCGCTGTCCGCCG
ATAGCGCTCGCATGCAGCTCGGCGGCTACACGCTGTCGCGCGCGACGCTCACCGTCGCCGACGGCGGCGCGTCGCACGCG
TTCAGCGCGAAGGAGTTCGACATTCTCTGGCATCTGGCCGAGCATCCGGGCGCCGTCGTGCAGCGGCAGGACCTGCTGCG
GCTCGTGTGGGGCGCGGAAGCGTCCGCGCAGACCCGCACGGTCGACATGTACGTGAGCCGGCTGCGCAGCCGGCTGAAGG
CGGCGGGCATCGGCTGGACCGTGCATGCGGCGTACGCGACCGGCTACCGCCTGAACCTCGGCGCGGACGCGGAAACGGCG
GACCGCGCGTCGTGA

Upstream 100 bases:

>100_bases
CGCGCTGGCGCGCGAGCGTCGCGCCGGACGGCTGAGCGCGCGGGTGCGCGTATTTCCTTTTTCGCTTGAAGATTCTTTTC
TTACGATGTCCGAACCGAAC

Downstream 100 bases:

>100_bases
CGCGGCGCGCGGCCGCATCGGCTGCCGCCGTCGCGCTCGCGCTCGCGAGCCATGCGGCGGCGCTGCGGGCGGTGCCGCTC
GCGTGGCCGCTCGCGCGTTT

Product: two-component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MQRTVQQPKQIVVVEDDPVQRTLLVTWLKAEGYRVEAFDDGLDARRFLGDSWADLLLLDWDLPGMTGERLLAWVRGRARS
IVPVIFQTVHSDEEDIVKILDAGADDFLIKPLERQTLLARVRALLRRFAALSADSARMQLGGYTLSRATLTVADGGASHA
FSAKEFDILWHLAEHPGAVVQRQDLLRLVWGAEASAQTRTVDMYVSRLRSRLKAAGIGWTVHAAYATGYRLNLGADAETA
DRAS

Sequences:

>Translated_244_residues
MQRTVQQPKQIVVVEDDPVQRTLLVTWLKAEGYRVEAFDDGLDARRFLGDSWADLLLLDWDLPGMTGERLLAWVRGRARS
IVPVIFQTVHSDEEDIVKILDAGADDFLIKPLERQTLLARVRALLRRFAALSADSARMQLGGYTLSRATLTVADGGASHA
FSAKEFDILWHLAEHPGAVVQRQDLLRLVWGAEASAQTRTVDMYVSRLRSRLKAAGIGWTVHAAYATGYRLNLGADAETA
DRAS
>Mature_244_residues
MQRTVQQPKQIVVVEDDPVQRTLLVTWLKAEGYRVEAFDDGLDARRFLGDSWADLLLLDWDLPGMTGERLLAWVRGRARS
IVPVIFQTVHSDEEDIVKILDAGADDFLIKPLERQTLLARVRALLRRFAALSADSARMQLGGYTLSRATLTVADGGASHA
FSAKEFDILWHLAEHPGAVVQRQDLLRLVWGAEASAQTRTVDMYVSRLRSRLKAAGIGWTVHAAYATGYRLNLGADAETA
DRAS

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=32, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787229, Length=215, Percent_Identity=33.4883720930233, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082012, Length=204, Percent_Identity=33.8235294117647, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789809, Length=210, Percent_Identity=32.8571428571429, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=29.6610169491525, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790860, Length=220, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=29.4642857142857, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=27.9279279279279, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786911, Length=207, Percent_Identity=32.8502415458937, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=26.7857142857143, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790552, Length=208, Percent_Identity=29.8076923076923, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI2367329, Length=211, Percent_Identity=34.5971563981043, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=27.5229357798165, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787487, Length=123, Percent_Identity=36.5853658536585, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693157, Length=111, Percent_Identity=35.1351351351351, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693140, Length=214, Percent_Identity=27.5700934579439, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=29.7520661157025, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27124; Mature: 27124

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTVQQPKQIVVVEDDPVQRTLLVTWLKAEGYRVEAFDDGLDARRFLGDSWADLLLLDW
CCCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEECCCCHHHHHHCCCCCCEEEEEC
DLPGMTGERLLAWVRGRARSIVPVIFQTVHSDEEDIVKILDAGADDFLIKPLERQTLLAR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCHHHHHHHHH
VRALLRRFAALSADSARMQLGGYTLSRATLTVADGGASHAFSAKEFDILWHLAEHPGAVV
HHHHHHHHHHCCCCCCEEEECCEEEECEEEEEECCCCCCCCCCCHHHHEEEECCCCCHHH
QRQDLLRLVWGAEASAQTRTVDMYVSRLRSRLKAAGIGWTVHAAYATGYRLNLGADAETA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEECCCCCCC
DRAS
CCCC
>Mature Secondary Structure
MQRTVQQPKQIVVVEDDPVQRTLLVTWLKAEGYRVEAFDDGLDARRFLGDSWADLLLLDW
CCCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEECCCCHHHHHHCCCCCCEEEEEC
DLPGMTGERLLAWVRGRARSIVPVIFQTVHSDEEDIVKILDAGADDFLIKPLERQTLLAR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCHHHHHHHHH
VRALLRRFAALSADSARMQLGGYTLSRATLTVADGGASHAFSAKEFDILWHLAEHPGAVV
HHHHHHHHHHCCCCCCEEEECCEEEECEEEEEECCCCCCCCCCCHHHHEEEECCCCCHHH
QRQDLLRLVWGAEASAQTRTVDMYVSRLRSRLKAAGIGWTVHAAYATGYRLNLGADAETA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEECCCCCCC
DRAS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]