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The map label for this gene is alaS [H]

Identifier: 53722342

GI number: 53722342

Start: 1804520

End: 1805239

Strand: Reverse

Name: alaS [H]

Synonym: BPSS1317

Alternate gene names: 53722342

Gene position: 1805239-1804520 (Counterclockwise)

Preceding gene: 53722343

Following gene: 53722341

Centisome position: 56.89

GC content: 69.31

Gene sequence:

>720_bases
ATGAATCGATTGTTCTGTCAAGACGACCCCGACACGCTGCTGCTTTCGGGCACGGTGGTCGACACGCGGCCGGGCGCGGT
GCGCTTGTCGCGGCATCCGTTCTATCCGGGCGGCGGCGGCCAGTTGCCCGATCTGGGCACGGTTCGATGGGAAGGCGGCG
TCGAGACCGTCGCCGGTTTCAGCGAGGACGGCGGCGGCCTCTGGATCGAATTCGCGAACGGCGTCGAGATCGTCGGCGAC
GTGCAGTCGAGCGTCGACGCCGCGTTCCGCCGCGCGATGCGCGAGCTGCATACCGATCTGCACGTGCTCAACGCGCTCGT
CTATCGCGATTTCGACGGCGCGCTCGTGACGGGCGTGCAGATGAACGCCGATCGCACCGCGCGCATCGATTTCGATCTGC
CCGACGCCGATAACGATCGCCTGCGCGCGCTGGAGGCCGAAATGAACGATGTCGTGCGCCGCGATCTGCCGGTGCGCACG
CGCTACGTGCCGCTCGCCGACGCGCAGCAGGCAAGCGGGCTGCTGCGCTCGCGCTCGGTCGCGCCGCCGCCGACGGCCGA
CGGGCTCGTGCGGATCGTCGAGATCGACGGGCTCGACAGCCAGGCGTGCGGCGGCACGCATCTCGCGTCGACGGGCGGCT
CGAGCCCGGTGCGGATCCTGAAGATCGAAAACAAGGGCCGCTCGAACCGCCGCGTGCGCATCGGGCTGGAGCCGAGATGA

Upstream 100 bases:

>100_bases
GAAGCGCCCGGCGGCGAGCGGGCGTCGCGCGCCGCCAAATTTTCGCCAATAAGCCCGCTTCGACTTCTGACATCTTTCAT
TCATCAGCCAGGTGGAGCTT

Downstream 100 bases:

>100_bases
CGCAGCGCCTGCTCGCGGCGGCATGGCGCCGGCCGGTGTGGCTGCTGTGGGTGCCGCCCGCGTTCTGGGCCGGCAACGTC
GTGCTCGGCCGCGCGTTCAG

Product: alanyl-tRNA synthetase related protein

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNRLFCQDDPDTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGFSEDGGGLWIEFANGVEIVGD
VQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQMNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRT
RYVPLADAQQASGLLRSRSVAPPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR

Sequences:

>Translated_239_residues
MNRLFCQDDPDTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGFSEDGGGLWIEFANGVEIVGD
VQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQMNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRT
RYVPLADAQQASGLLRSRSVAPPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR
>Mature_239_residues
MNRLFCQDDPDTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGFSEDGGGLWIEFANGVEIVGD
VQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQMNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRT
RYVPLADAQQASGLLRSRSVAPPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=231, Percent_Identity=28.1385281385281, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR022429
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 25911; Mature: 25911

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLFCQDDPDTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGF
CCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHCCC
SEDGGGLWIEFANGVEIVGDVQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQ
CCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
MNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRTRYVPLADAQQASGLLRSRSV
ECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCC
APPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR
CCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC
>Mature Secondary Structure
MNRLFCQDDPDTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGF
CCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHCCC
SEDGGGLWIEFANGVEIVGDVQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQ
CCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
MNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRTRYVPLADAQQASGLLRSRSV
ECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCC
APPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR
CCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA