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The map label for this gene is narI

Identifier: 53722184

GI number: 53722184

Start: 1541661

End: 1542344

Strand: Reverse

Name: narI

Synonym: BPSS1156

Alternate gene names: 53722184

Gene position: 1542344-1541661 (Counterclockwise)

Preceding gene: 53722185

Following gene: 53722183

Centisome position: 48.61

GC content: 66.52

Gene sequence:

>684_bases
ATGAGCGATTACCTGAATACGTTTCTGTTCGGCATCTATCCGTACATCTGTGTCGCGGTGCTGCTGATCGGCAGCCTGAT
CCGCTTCGATCGCGAGCAGTACACGTGGAAGAGCGACTCGTCGCAACTGCTGCGGCGCGGCGCGCTGCGGCTCGGCAGCA
ATCTGTTCCACTGGGGCGTGCTCGTCGTCGTGATCGGGCATTTCGTCGGCTTTCTCGCGCCGCACTGGCTCGTGTCGCCG
CTCATGTCGGCTTCGTCGCATCAACTGCTCGCGATGGTGGGCGGCGGCGTGGCGGGCGCGGTCGCGATCGCGGGCCTCAC
GATCCTGATCGCGCGGCGGCTCGGCGACGCACGCGTCCGGCGCAACAGCCGCAAGTCCGACATCCTGATCGTGCTGATGC
TGTGGGCGCAGCTCGCGCTCGGGCTCGGCACCGTCGCGCTGTCCGCGCGCCACATGGACGGCGCGATGTTCGAGGCGCTG
ACCGACTACGTGAAGGGCATCGTCACGTTCCAGCCGGGCGTCGCGGCGCTCGTCGCCGGCGTGCCGTGGACTTATCGCGC
GCACATCCTGCTCGGCTTCACGCTCTTCCTCGTGTCGCCGTTCACGCGGATGGTGCATGTCTGGAGCGGATTCGCGTCGG
TCGCCTATCTGATCCGCCCTTACCAGCTCGTTCGCAAGCGCTGA

Upstream 100 bases:

>100_bases
GCGAGGCGAGGTGAGGTGCAGCGAGGCGAGACGCAGCGAAAGGCCGGCGGCGTCGAAAGCGGTCGGGGCGAGGCGCCCGG
CGAGGCATCAGGAGAGGCCC

Downstream 100 bases:

>100_bases
TCGGCGGCGGACCCGGCCGATGGATACGATCGACGAAACCCTCGAGGCGCGGCCGCACGCGCGCGTGAGCGTCAACGGCG
TCGAGATCGGCGCGGCCGAG

Product: respiratory nitrate reductase subunit

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSDYLNTFLFGIYPYICVAVLLIGSLIRFDREQYTWKSDSSQLLRRGALRLGSNLFHWGVLVVVIGHFVGFLAPHWLVSP
LMSASSHQLLAMVGGGVAGAVAIAGLTILIARRLGDARVRRNSRKSDILIVLMLWAQLALGLGTVALSARHMDGAMFEAL
TDYVKGIVTFQPGVAALVAGVPWTYRAHILLGFTLFLVSPFTRMVHVWSGFASVAYLIRPYQLVRKR

Sequences:

>Translated_227_residues
MSDYLNTFLFGIYPYICVAVLLIGSLIRFDREQYTWKSDSSQLLRRGALRLGSNLFHWGVLVVVIGHFVGFLAPHWLVSP
LMSASSHQLLAMVGGGVAGAVAIAGLTILIARRLGDARVRRNSRKSDILIVLMLWAQLALGLGTVALSARHMDGAMFEAL
TDYVKGIVTFQPGVAALVAGVPWTYRAHILLGFTLFLVSPFTRMVHVWSGFASVAYLIRPYQLVRKR
>Mature_226_residues
SDYLNTFLFGIYPYICVAVLLIGSLIRFDREQYTWKSDSSQLLRRGALRLGSNLFHWGVLVVVIGHFVGFLAPHWLVSPL
MSASSHQLLAMVGGGVAGAVAIAGLTILIARRLGDARVRRNSRKSDILIVLMLWAQLALGLGTVALSARHMDGAMFEALT
DYVKGIVTFQPGVAALVAGVPWTYRAHILLGFTLFLVSPFTRMVHVWSGFASVAYLIRPYQLVRKR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=225, Percent_Identity=46.2222222222222, Blast_Score=206, Evalue=8e-55,
Organism=Escherichia coli, GI1787738, Length=227, Percent_Identity=44.0528634361233, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25045; Mature: 24914

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLNTFLFGIYPYICVAVLLIGSLIRFDREQYTWKSDSSQLLRRGALRLGSNLFHWGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHH
LVVVIGHFVGFLAPHWLVSPLMSASSHQLLAMVGGGVAGAVAIAGLTILIARRLGDARVR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNSRKSDILIVLMLWAQLALGLGTVALSARHMDGAMFEALTDYVKGIVTFQPGVAALVAG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
VPWTYRAHILLGFTLFLVSPFTRMVHVWSGFASVAYLIRPYQLVRKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDYLNTFLFGIYPYICVAVLLIGSLIRFDREQYTWKSDSSQLLRRGALRLGSNLFHWGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHH
LVVVIGHFVGFLAPHWLVSPLMSASSHQLLAMVGGGVAGAVAIAGLTILIARRLGDARVR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNSRKSDILIVLMLWAQLALGLGTVALSARHMDGAMFEALTDYVKGIVTFQPGVAALVAG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
VPWTYRAHILLGFTLFLVSPFTRMVHVWSGFASVAYLIRPYQLVRKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]