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The map label for this gene is narK

Identifier: 53722182

GI number: 53722182

Start: 1539545

End: 1540819

Strand: Reverse

Name: narK

Synonym: BPSS1154

Alternate gene names: 53722182

Gene position: 1540819-1539545 (Counterclockwise)

Preceding gene: 53722183

Following gene: 53722175

Centisome position: 48.56

GC content: 68.86

Gene sequence:

>1275_bases
ATGGGCTCACGGCACGGTTCCGGCGCGTTCGACCCCGCGCCCGCGATTCCCGCACGCGCCTGGTCGGTGCTCTTCGCGAG
CACGCTCGCGTTTCTCGTGTGTTTCGTCGTCTGGATGATGTTCGGCGTGCTCGGCGTCCCGTTGCGCGAGGATCTGCATC
TGAATTCGACCGAGTTCGGGCTCCTCACGTCGACGCCCGTGCTGACGGGCGCGCTGATGCGGCTGCCGCTCGGCGCATGG
ACCGACCGCTTCGGCGGGCGCATCGTGATGACGGTGCTGCTCGTCGTCTGCTCGGTGCCCGTCTATGGGGTCAGCTATGC
GAACGCGCTGTGGCAGTTTCTGCTGATCGGGCTGTTCCTCGGCTGCGTCGGCGCGTCGTTCGCGGTCGGCACGCCATACG
TCGCGCGTTTCTTCCCGCCGCGGCGGCGCGGGCTCGCGATGGGCGTGTTCGGCGCGGGCACCGTGGGCGCGGCGGTGAAC
CTGTTCGTCACGCCGCTCTTGCTCGCCGCGTACGGCTGGCGCGTCGTGCCGCGCGTCTACGCGCTCGCGCTCGTCGTCAC
CGCCGCGGTCTTCTGGTTCGCGTCGGCGCCCGATCCCGGCGCGGGCGAGCAGAAGGGCTCATGGGTCGATTCGTTCAAGG
TGCTGCGCGATCCGCGCGTCTGGAAGCTTTGCCAGTACTACTCGATCACGTTCGGCGGCTTCACCGCGCTGTCGCTGTGG
ATTCCGCAGTACCTGAAGAACGGCTATGGGATGTCGCTCGTCGCGGCTTCCGCGTTCGCGGCGGGCTTTTCGCTGCCGGG
CTCGGTGCTGCGCGCGCTCGGCGGCGCGCTCGCCGACCGCTACGGCGCGCACGCGGTCACCTGGTGGGGGCTGTGGGTCG
CGTGGATTTGCCTGTTCCTGCTGTCCTATCCGCCGACCGACCTCGTGATTCACACGATCGACGGCACCGCGACGCTGCGG
CTTCATGTGCCGCTCGCGGCGTTCGTCGTGCTGACGTTCGTGCTGGGCGCGGTGTTCGCGTTCGGGATGGCGTCGACGTT
CAAGTACGTCGCCGACGATTTCCCGAACAACATGGGCGTCGTCACCGGCATCGTCGGGCTCGCGGGCGGGCTCGGCGGCT
TTCTGCTGCCGATCATCTTCGGCGCGCTCGTCGATCTGCTGCGCGTGCGCTCGACGTGCTTCATGTTCCTGTACGGGATC
GTCTGGGTGTCGCTGATCCTGCTGTACCTCGCGGAAATCCGGCGCGCGCCCGTCACGGGCGCGCCCCCGCGCTGA

Upstream 100 bases:

>100_bases
CGTGACGATTCTCGCGAGCGGCGCGCGGATCGAAGGCGTCGCGCTCGGCGACGCGAACGGCCCGCTGGTTCAATGAGCTG
ACTTCACAAGGAGGGCGCAC

Downstream 100 bases:

>100_bases
CGAATCAGGCGAGGCGCTCGGCGCCGGCCGGCGGGGCGGCCGGCCTGGCCTCGGCAGCCGCGGCATCGGCCGGCTTGCGC
CCGCGCGCGACGGGTTCGAG

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGSRHGSGAFDPAPAIPARAWSVLFASTLAFLVCFVVWMMFGVLGVPLREDLHLNSTEFGLLTSTPVLTGALMRLPLGAW
TDRFGGRIVMTVLLVVCSVPVYGVSYANALWQFLLIGLFLGCVGASFAVGTPYVARFFPPRRRGLAMGVFGAGTVGAAVN
LFVTPLLLAAYGWRVVPRVYALALVVTAAVFWFASAPDPGAGEQKGSWVDSFKVLRDPRVWKLCQYYSITFGGFTALSLW
IPQYLKNGYGMSLVAASAFAAGFSLPGSVLRALGGALADRYGAHAVTWWGLWVAWICLFLLSYPPTDLVIHTIDGTATLR
LHVPLAAFVVLTFVLGAVFAFGMASTFKYVADDFPNNMGVVTGIVGLAGGLGGFLLPIIFGALVDLLRVRSTCFMFLYGI
VWVSLILLYLAEIRRAPVTGAPPR

Sequences:

>Translated_424_residues
MGSRHGSGAFDPAPAIPARAWSVLFASTLAFLVCFVVWMMFGVLGVPLREDLHLNSTEFGLLTSTPVLTGALMRLPLGAW
TDRFGGRIVMTVLLVVCSVPVYGVSYANALWQFLLIGLFLGCVGASFAVGTPYVARFFPPRRRGLAMGVFGAGTVGAAVN
LFVTPLLLAAYGWRVVPRVYALALVVTAAVFWFASAPDPGAGEQKGSWVDSFKVLRDPRVWKLCQYYSITFGGFTALSLW
IPQYLKNGYGMSLVAASAFAAGFSLPGSVLRALGGALADRYGAHAVTWWGLWVAWICLFLLSYPPTDLVIHTIDGTATLR
LHVPLAAFVVLTFVLGAVFAFGMASTFKYVADDFPNNMGVVTGIVGLAGGLGGFLLPIIFGALVDLLRVRSTCFMFLYGI
VWVSLILLYLAEIRRAPVTGAPPR
>Mature_423_residues
GSRHGSGAFDPAPAIPARAWSVLFASTLAFLVCFVVWMMFGVLGVPLREDLHLNSTEFGLLTSTPVLTGALMRLPLGAWT
DRFGGRIVMTVLLVVCSVPVYGVSYANALWQFLLIGLFLGCVGASFAVGTPYVARFFPPRRRGLAMGVFGAGTVGAAVNL
FVTPLLLAAYGWRVVPRVYALALVVTAAVFWFASAPDPGAGEQKGSWVDSFKVLRDPRVWKLCQYYSITFGGFTALSLWI
PQYLKNGYGMSLVAASAFAAGFSLPGSVLRALGGALADRYGAHAVTWWGLWVAWICLFLLSYPPTDLVIHTIDGTATLRL
HVPLAAFVVLTFVLGAVFAFGMASTFKYVADDFPNNMGVVTGIVGLAGGLGGFLLPIIFGALVDLLRVRSTCFMFLYGIV
WVSLILLYLAEIRRAPVTGAPPR

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=289, Percent_Identity=27.3356401384083, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787743, Length=410, Percent_Identity=25.8536585365854, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45501; Mature: 45370

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure 
GSRHGSGAFDPAPAIPARAWSVLFASTLAFLVCFVVWMMFGVLGVPLREDLHLNSTEFG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC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CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]