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The map label for this gene is thyA [C]

Identifier: 53722134

GI number: 53722134

Start: 1484863

End: 1485852

Strand: Reverse

Name: thyA [C]

Synonym: BPSS1106

Alternate gene names: 53722134

Gene position: 1485852-1484863 (Counterclockwise)

Preceding gene: 53722135

Following gene: 53722133

Centisome position: 46.83

GC content: 67.98

Gene sequence:

>990_bases
ATGCTGCGCTATCCGGGTCTCAGCGAGCTCTACCTCGCGACGCTCAGGGACGTCTGCGGCGCGTACGAATACCGGAGCGC
GCCGCGCGGGCACGCGGAGCGCGAAATCATCGGCTACGGCGCACGGATCGACGATCCGCGCGCGCGCTTTTGCCGGCATG
CGGCGCGCAAGCAGAACGTCGTCTTCAACTACGCCGAGGCGCTATGGTATCTGTCCGGGCGCAACGATCTCGAATTCATC
GCGCACTACGCACCGTCGATGGCGCGCTACAGTCCGGACGGCAAGCATCTGCCCGGCACCGGCTACGGCGCGCGCCTGCT
GCATTACGGCGAGCGCGGGCTCGACCAGATCGAGCGCGCGCTCGACATCCTGTCGCGCGACGACGCCGAGAGCAAGCGCG
TCGTGCTGCAGATCTTCAGCGCCGACGAGGAACTGTACCGGCGCAACATCGACGTGTCGTGCACGCTCGGCCTGCAACTG
CTGCTGCGCGAAGGCCGGCTGCACATGGTCGCGTTCATGCGCGCGAACGACGCGTACATCGGGCTGCTGAACGACGTGTT
CTCGTTCACGTTCCTGCAGGAGTACCTCGCGTCGCGGCTCGGCTGCGAGCTCGGCACGTATGCGCATCAGGTGGGCTCGA
TCCACGTGTACGACGACAATCTGCCGCGCGTGCAGGCGCTGCTCGACGACAGCGATGCGGCGATCGTCACCGCCGCCGCG
CCGCCGCGCATGCCGGCGGGCACGCGCGCCGCGACGATCGCGCGCGTGCTCGAGCACGAAGGCCGGATTCGCGCCGGGCG
CGTGTCGCTCGCCGCGCTTCGCGACATCGATCTCGATCCGTACTGGCGCGACGTGCTCGGTCTCTTCTGGATCTATCGCG
AGATCAAGGCGGGCAACCCGCCGCCCGACGAGGCGCTCGCGTTCGTCGGCCCGTTCCATCGCGCCTATCTGATGAACCGG
TGGGACATGGCGTCCGCCTCGCTCCAATAA

Upstream 100 bases:

>100_bases
GACGAGATCACGCGCATCGAACGCGTCGAGTTCAGCGGCGACCGGATCGATCCGGTCGCGATCGAAGCCGCGATCGGCCG
TTTCGACGCACGAGGCTGAC

Downstream 100 bases:

>100_bases
CGCACGGGGATCCCGATGAAGACCGACTGGACCGAACACGAGGTTTCGCAGCGCTTTCACGCATACGACGACCGGATCGA
GGCGCGCTTCGGCTACCAGC

Product: thymidylate synthase protein

Products: 7,8-Dihydrofolate; dTMP [C]

Alternate protein names: Thymidylate Synthase Protein; Deoxyuridylate Hydroxymethyltransferase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLRYPGLSELYLATLRDVCGAYEYRSAPRGHAEREIIGYGARIDDPRARFCRHAARKQNVVFNYAEALWYLSGRNDLEFI
AHYAPSMARYSPDGKHLPGTGYGARLLHYGERGLDQIERALDILSRDDAESKRVVLQIFSADEELYRRNIDVSCTLGLQL
LLREGRLHMVAFMRANDAYIGLLNDVFSFTFLQEYLASRLGCELGTYAHQVGSIHVYDDNLPRVQALLDDSDAAIVTAAA
PPRMPAGTRAATIARVLEHEGRIRAGRVSLAALRDIDLDPYWRDVLGLFWIYREIKAGNPPPDEALAFVGPFHRAYLMNR
WDMASASLQ

Sequences:

>Translated_329_residues
MLRYPGLSELYLATLRDVCGAYEYRSAPRGHAEREIIGYGARIDDPRARFCRHAARKQNVVFNYAEALWYLSGRNDLEFI
AHYAPSMARYSPDGKHLPGTGYGARLLHYGERGLDQIERALDILSRDDAESKRVVLQIFSADEELYRRNIDVSCTLGLQL
LLREGRLHMVAFMRANDAYIGLLNDVFSFTFLQEYLASRLGCELGTYAHQVGSIHVYDDNLPRVQALLDDSDAAIVTAAA
PPRMPAGTRAATIARVLEHEGRIRAGRVSLAALRDIDLDPYWRDVLGLFWIYREIKAGNPPPDEALAFVGPFHRAYLMNR
WDMASASLQ
>Mature_329_residues
MLRYPGLSELYLATLRDVCGAYEYRSAPRGHAEREIIGYGARIDDPRARFCRHAARKQNVVFNYAEALWYLSGRNDLEFI
AHYAPSMARYSPDGKHLPGTGYGARLLHYGERGLDQIERALDILSRDDAESKRVVLQIFSADEELYRRNIDVSCTLGLQL
LLREGRLHMVAFMRANDAYIGLLNDVFSFTFLQEYLASRLGCELGTYAHQVGSIHVYDDNLPRVQALLDDSDAAIVTAAA
PPRMPAGTRAATIARVLEHEGRIRAGRVSLAALRDIDLDPYWRDVLGLFWIYREIKAGNPPPDEALAFVGPFHRAYLMNR
WDMASASLQ

Specific function: Provides The Sole De Novo Source Of Dtmp For DNA Biosynthesis. This Protein Also Binds To Its Mrna Thus Repressing Its Own Translation. [C]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.45 [C]

Molecular weight: Translated: 37168; Mature: 37168

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRYPGLSELYLATLRDVCGAYEYRSAPRGHAEREIIGYGARIDDPRARFCRHAARKQNV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHCCH
VFNYAEALWYLSGRNDLEFIAHYAPSMARYSPDGKHLPGTGYGARLLHYGERGLDQIERA
HEEHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LDILSRDDAESKRVVLQIFSADEELYRRNIDVSCTLGLQLLLREGRLHMVAFMRANDAYI
HHHHHCCCCCHHEEEHHHHHCCHHHHHCCCCEEEHHHHHHHHHCCCEEEEEEEECCCCEE
GLLNDVFSFTFLQEYLASRLGCELGTYAHQVGSIHVYDDNLPRVQALLDDSDAAIVTAAA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEECCCCCHHHHHHCCCCCEEEEECC
PPRMPAGTRAATIARVLEHEGRIRAGRVSLAALRDIDLDPYWRDVLGLFWIYREIKAGNP
CCCCCCCCHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
PPDEALAFVGPFHRAYLMNRWDMASASLQ
CHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLRYPGLSELYLATLRDVCGAYEYRSAPRGHAEREIIGYGARIDDPRARFCRHAARKQNV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHCCH
VFNYAEALWYLSGRNDLEFIAHYAPSMARYSPDGKHLPGTGYGARLLHYGERGLDQIERA
HEEHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LDILSRDDAESKRVVLQIFSADEELYRRNIDVSCTLGLQLLLREGRLHMVAFMRANDAYI
HHHHHCCCCCHHEEEHHHHHCCHHHHHCCCCEEEHHHHHHHHHCCCEEEEEEEECCCCEE
GLLNDVFSFTFLQEYLASRLGCELGTYAHQVGSIHVYDDNLPRVQALLDDSDAAIVTAAA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEECCCCCHHHHHHCCCCCEEEEECC
PPRMPAGTRAATIARVLEHEGRIRAGRVSLAALRDIDLDPYWRDVLGLFWIYREIKAGNP
CCCCCCCCHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
PPDEALAFVGPFHRAYLMNRWDMASASLQ
CHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dithiothreitol [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dUMP; 5,10-Methylenetetrahydrofolate [C]

Specific reaction: dUMP + 5,10-Methylenetetrahydrofolate --> 7,8-Dihydrofolate + dTMP [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5'- monophosphate; 10-Formyl dihydrofolyl heptaglutamate; 10-Formyl dihydrofolyl monoglutamate; 10-Formyl tetrahydrofolyl heptaglutamate; 10-Formyl tetrahydrofolyl monoglutamate; Amethopterin; Dithiothreitol [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA