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The map label for this gene is mcbR [H]

Identifier: 53721791

GI number: 53721791

Start: 1026819

End: 1027535

Strand: Reverse

Name: mcbR [H]

Synonym: BPSS0765

Alternate gene names: 53721791

Gene position: 1027535-1026819 (Counterclockwise)

Preceding gene: 53721793

Following gene: 53721790

Centisome position: 32.38

GC content: 70.99

Gene sequence:

>717_bases
TTGGCGCCGCGTATCGTCCCGCCCGCCCTCGAGGCCATCGAACAGGAAACGATGACCGACAAGGTCTACCAGCAACTCCG
CGAGGCGCTGATGAGCGGGCGCTTCGCGCCCGGCCAGGCGCTCAGCCTGCGCAGCGTCGCCGAGGCCGTCGGCTCGTCGA
CGATGCCCGTGCGCGCCGCGCTCACGCGGCTGCGCGCGGAGCGCGCGCTCGCCGACGGCCCGAATCGCGCGCTCGTCGTG
CCGCCGATGACGATCGAGATGCTCGATGAGTTGCGCGACGTGCGGATCGCGCTCGAAGGCTGCGTCGCCGAACGCGCGGC
GACACGGATGAGCGTCTCGCAGATCGCCGAGGTGCGGCGCATCTGCGAGGCGATGAACGCGCACGTCGAAGCGGGCCGAA
GCCGCGCGTATCTGCAAAGCAATTTCGCGTTCCATAGCGCGATCTACGCGCACGGCGCGAGCGAGAACACGCTCGCGATC
ATTCGCAATCTATGGATGCGGATCGGCCCGTTCCTGAACATGATCGCGCTCGACGTGCCGCACATGCGGCGTTCGATGGA
CGCGCACCGCGCGATCGTCGACGCGCTCGAGCGCCGCGACGGCGCCGGCGCGCGCGCGGGCATCGCGCTCGACATCGGCG
GCGCCGCGCACGATCTCGCCGAGACGCTCGCGGCCGAACGCGCGCGTCTCGGCCCGCGCCGGCTCGCGAACGAATAG

Upstream 100 bases:

>100_bases
CCTGATCGTCGCGCGCTTGACTGCGGCCGTCGCATCGACAACACTGCGTAACATCTCAAGTGTGATCACGGATCACAGAT
CAAACCCAAAGGAGTTCGTC

Downstream 100 bases:

>100_bases
CGGCGCGCCGCGCGCATGCGGCGCCGCCCGCCTCGCGCGCGCATCACCAATATCGCGATGCGCATCGTGCGCATCGCCGA
ACAAGAATCGCGATCACTCG

Product: DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MAPRIVPPALEAIEQETMTDKVYQQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAALTRLRAERALADGPNRALVV
PPMTIEMLDELRDVRIALEGCVAERAATRMSVSQIAEVRRICEAMNAHVEAGRSRAYLQSNFAFHSAIYAHGASENTLAI
IRNLWMRIGPFLNMIALDVPHMRRSMDAHRAIVDALERRDGAGARAGIALDIGGAAHDLAETLAAERARLGPRRLANE

Sequences:

>Translated_238_residues
MAPRIVPPALEAIEQETMTDKVYQQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAALTRLRAERALADGPNRALVV
PPMTIEMLDELRDVRIALEGCVAERAATRMSVSQIAEVRRICEAMNAHVEAGRSRAYLQSNFAFHSAIYAHGASENTLAI
IRNLWMRIGPFLNMIALDVPHMRRSMDAHRAIVDALERRDGAGARAGIALDIGGAAHDLAETLAAERARLGPRRLANE
>Mature_237_residues
APRIVPPALEAIEQETMTDKVYQQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAALTRLRAERALADGPNRALVVP
PMTIEMLDELRDVRIALEGCVAERAATRMSVSQIAEVRRICEAMNAHVEAGRSRAYLQSNFAFHSAIYAHGASENTLAII
RNLWMRIGPFLNMIALDVPHMRRSMDAHRAIVDALERRDGAGARAGIALDIGGAAHDLAETLAAERARLGPRRLANE

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=204, Percent_Identity=28.4313725490196, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25938; Mature: 25807

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRIVPPALEAIEQETMTDKVYQQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
LTRLRAERALADGPNRALVVPPMTIEMLDELRDVRIALEGCVAERAATRMSVSQIAEVRR
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICEAMNAHVEAGRSRAYLQSNFAFHSAIYAHGASENTLAIIRNLWMRIGPFLNMIALDVP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
HMRRSMDAHRAIVDALERRDGAGARAGIALDIGGAAHDLAETLAAERARLGPRRLANE
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
APRIVPPALEAIEQETMTDKVYQQLREALMSGRFAPGQALSLRSVAEAVGSSTMPVRAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
LTRLRAERALADGPNRALVVPPMTIEMLDELRDVRIALEGCVAERAATRMSVSQIAEVRR
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICEAMNAHVEAGRSRAYLQSNFAFHSAIYAHGASENTLAIIRNLWMRIGPFLNMIALDVP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
HMRRSMDAHRAIVDALERRDGAGARAGIALDIGGAAHDLAETLAAERARLGPRRLANE
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]