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The map label for this gene is pchA

Identifier: 53721617

GI number: 53721617

Start: 790031

End: 791485

Strand: Reverse

Name: pchA

Synonym: BPSS0581

Alternate gene names: 53721617

Gene position: 791485-790031 (Counterclockwise)

Preceding gene: 53721618

Following gene: 53721610

Centisome position: 24.94

GC content: 74.71

Gene sequence:

>1455_bases
ATGAGCGCCCGCCTGTCCGCGCTGCTCGATACGTTCGCGCGCGCCGCGCGCCGCGCGGCCGACACGGGCGAGCCCGTGCT
CGCCGCGGCATCGTTTCCGCTGCGGCCGCTGAACCTGTTCGATCTGATCGCGAGCTGGGACGACGGCGCAACCCCATGGT
GCTTCGTCGAAGCCGGCGACGAGCCGGGCGCGATGCGCGCGCCGGGCAAGCCGCCCGCCCTGTTCGGCTGGGATTGCGCA
CTCGATCTGAACGAGGCGGGCGACGCGCGATTCGCCCGGCTCGACGCGCGCTGGCGCGCGCTCGTGCGCGCGGCGGTCGT
CACCGGCGCGCAGCCGCCGCGCATCGTCGGCGGCTTCCGCTTCGATCCGCTCGGGCCGCGATACGCGCACTGGCACGCGT
TCGCCGATGCGAGCATGACGCTTGCGAAACTGCTGGTCGTCCGCGAAGGCGAGGCGTATTGGGCGGTCTGCCAGCGCGTC
GTCGCCGCGCACGACGATCCCGCCGCGCTCGCGCGCGAATGCGACGCGCGCATCGGCATGCTCGCGTCGCTCGCGCCCGC
CGCCGACGACGAGGCGCCGCGCCTGCTGCGCGTTCAGGCGCTGCAGGCCGCCGCATGGCAGCACGAGGTGCGGCGCGCGG
TCGACGCGATCCGCGGCGAGGCGTTCAGCAAGGTCGTGCTCGCGCGCGACGTGCTGCAACAGTACGCGCGGCCCGTGGCG
ATCGGGCCGCTGCTGCGCCGGCTCAGGCGGCGCGACCCGCGCGCGCACCTGTTCGCGATCCGCAGGCGCGACGGCTGCTT
CGTCGGCGCGACGCCGGAACGCCTCGCGCGCGTCGCGCACGGCCGCGCGCACACGCACGCGCTCGCCGGCACGATCGCGC
GCGGCAACGATCCGGCTCACGATCGCGCGCTCGGCGCGCAGCTGATGGCGTCGGCGAAAGAGCGCCTCGAGCACGCGCTC
GTGGTCGACGCGATTCGCGATGCGCTCGCGCCGCTGTCGCGCGCGATCGATGTGCCCGCGCAGCCGTCGCTATTGCGGCT
GCCGCGCCTGCAACATCTGAGCACGCCGATCGCCGCGGCGCTGAACGAGCGCGCGACGCTGCTGCAAGTCGTCGCGGCGC
TGCATCCGACGCCCGCCGTCGCGGGCCATCCCCGCGCCGCCGCGCTCGAGCACATTCGCGCGCACGAAGGCTTCGATCGC
GGCTGGTATGCGGGCCCGATCGGCTGGATCGACGCGCACGGCAACGGCGACTTCGTCGTCGCGCTGCGCTCGGCGCTGAT
CTCGGCCGGTGCCTGCCGGCTGTTCGCCGGCTGCGGGATCGTCGCCGCATCGGAGCCGGCGCGCGAATATCGCGAAACGA
GCCTCAAGCTGTCGGGGATGCAGGCGGCGATCGAGGCGCGCGACGCCGCAGCAGCAGCGCGCACGGTCGCGCCGATTCCG
TCCGCCGCGCACTGA

Upstream 100 bases:

>100_bases
CCGGGCTCGACGGCGCGTTCGTCGAGGCGCTGTATGCGCAAGTCATCGCGTGGTACATCGAACAGCAAACGCGGCACTGG
CGCCGCCAGCGGGGGCTCGC

Downstream 100 bases:

>100_bases
CGAGGCATCGATGCGGCGCGCGCGCCGCGCGGCGCCGAATCGCGCCCAAACCGCGCCGCGCGGTGCGGCGCGCCACGCAT
CGCGCGACAAGCCGGGAAAA

Product: salicylate biosynthesis isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSARLSALLDTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGDEPGAMRAPGKPPALFGWDCA
LDLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEAYWAVCQRV
VAAHDDPAALARECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVLQQYARPVA
IGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGRAHTHALAGTIARGNDPAHDRALGAQLMASAKERLEHAL
VVDAIRDALAPLSRAIDVPAQPSLLRLPRLQHLSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHIRAHEGFDR
GWYAGPIGWIDAHGNGDFVVALRSALISAGACRLFAGCGIVAASEPAREYRETSLKLSGMQAAIEARDAAAAARTVAPIP
SAAH

Sequences:

>Translated_484_residues
MSARLSALLDTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGDEPGAMRAPGKPPALFGWDCA
LDLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEAYWAVCQRV
VAAHDDPAALARECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVLQQYARPVA
IGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGRAHTHALAGTIARGNDPAHDRALGAQLMASAKERLEHAL
VVDAIRDALAPLSRAIDVPAQPSLLRLPRLQHLSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHIRAHEGFDR
GWYAGPIGWIDAHGNGDFVVALRSALISAGACRLFAGCGIVAASEPAREYRETSLKLSGMQAAIEARDAAAAARTVAPIP
SAAH
>Mature_483_residues
SARLSALLDTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGDEPGAMRAPGKPPALFGWDCAL
DLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEAYWAVCQRVV
AAHDDPAALARECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVLQQYARPVAI
GPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGRAHTHALAGTIARGNDPAHDRALGAQLMASAKERLEHALV
VDAIRDALAPLSRAIDVPAQPSLLRLPRLQHLSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHIRAHEGFDRG
WYAGPIGWIDAHGNGDFVVALRSALISAGACRLFAGCGIVAASEPAREYRETSLKLSGMQAAIEARDAAAAARTVAPIPS
AAH

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=260, Percent_Identity=38.8461538461538, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI87082077, Length=263, Percent_Identity=36.5019011406844, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788114, Length=208, Percent_Identity=29.3269230769231, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787518, Length=221, Percent_Identity=31.2217194570136, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=348, Percent_Identity=27.2988505747126, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51821; Mature: 51690

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARLSALLDTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGD
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
EPGAMRAPGKPPALFGWDCALDLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFR
CCCCCCCCCCCCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
FDPLGPRYAHWHAFADASMTLAKLLVVREGEAYWAVCQRVVAAHDDPAALARECDARIGM
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVLQQYARPVA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGRAHTHALAGTIARGNDPAH
HHHHHHHHHCCCCCHHEEEEEECCCEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
DRALGAQLMASAKERLEHALVVDAIRDALAPLSRAIDVPAQPSLLRLPRLQHLSTPIAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHCCHHHHH
LNERATLLQVVAALHPTPAVAGHPRAAALEHIRAHEGFDRGWYAGPIGWIDAHGNGDFVV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHH
ALRSALISAGACRLFAGCGIVAASEPAREYRETSLKLSGMQAAIEARDAAAAARTVAPIP
HHHHHHHHHCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAAH
CCCC
>Mature Secondary Structure 
SARLSALLDTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGD
CHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
EPGAMRAPGKPPALFGWDCALDLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFR
CCCCCCCCCCCCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
FDPLGPRYAHWHAFADASMTLAKLLVVREGEAYWAVCQRVVAAHDDPAALARECDARIGM
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVLQQYARPVA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGRAHTHALAGTIARGNDPAH
HHHHHHHHHCCCCCHHEEEEEECCCEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
DRALGAQLMASAKERLEHALVVDAIRDALAPLSRAIDVPAQPSLLRLPRLQHLSTPIAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHCCHHHHH
LNERATLLQVVAALHPTPAVAGHPRAAALEHIRAHEGFDRGWYAGPIGWIDAHGNGDFVV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHH
ALRSALISAGACRLFAGCGIVAASEPAREYRETSLKLSGMQAAIEARDAAAAARTVAPIP
HHHHHHHHHCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAAH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]