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The map label for this gene is citH [H]

Identifier: 53721183

GI number: 53721183

Start: 192341

End: 193654

Strand: Reverse

Name: citH [H]

Synonym: BPSS0147

Alternate gene names: 53721183

Gene position: 193654-192341 (Counterclockwise)

Preceding gene: 53721184

Following gene: 53721182

Centisome position: 6.1

GC content: 71.39

Gene sequence:

>1314_bases
ATGGAAACGTCCGCTCTCAATTTCGCCGGCGCGCCGGCCGACGCGCGCGCCGCCGCGAAGAAACGCCGCCTGATCGCCGC
GGCCGCCGTCGGCAACGCGCTCGAGTTCTACGATTTCACCGTCTACAGCTTCTTCGCGATCCTGATCGGCAAGCTGTTCT
TCCCGGTTCACTCGCCGTTCGGGCAGTTGATGCTCGCGGTCGCGAGCTTCGGCGTGGGGTTCGTCACGCGCCCGCTCGGC
GGGCTCGTGATCGGCGTCTACGCGGATCGCGCCGGGCGCAAGCGCGCGATGATCGCAACGCTGCTCATCATGGCGCTCGG
CACCGCGACGATCGCCGTCGCGCCGACTTACGCGCAGATCGGCATCGCGGCGCCGCTGCTGCTCGTGGCCGCGCGCTTGC
TGCAGGGGTTCGCGTCCGGCGGCGAGGTCGGCGCGTCCACCACGCTGCTGATCGAGCAGGCGCCGCAGGCGCGGCGCGGC
TTCTATGCGTCGTTCCAGTTCTCGAGCCAGGGGCTCGCCGCGCTCGCCGGCGCGCTCACCGGCGTGCTGCTCACGTCGAC
GCTCAGCGCCGCGCAGCTCGAGAGCTGGGGGTGGCGCGTGCCGTTCGTCATCGGCACGCTGTTCGCGCCGCTCGGCTACT
GGCTGCGGCGCACGGTCGACGAGGCGCCGGCGGCCGCGCCCGCGCCGCGCGGCGACGCCGCGCCGCTGCCGCTCGCCGAC
GTGCTGCGCCATCACGGCAAGGCCGTATTCGCCGGCCTCGGCATCACGATCGGCGGCACGTCGATCCACTACATCATCGT
GTTCTACATGGCGATCTACGGCGTGCAGGTGCTGCATCTGCCGAGCTGGCTGTCGATGTCGGCGGGCTGCATCGCCGGCG
CGATCCTCGCGGTGGTCACGCCGATCGGCGGCCATCTGTCGGACCGCTACGGGCGCAACCGGATCGTCTGGTGGACGCGC
GTCGCGCTGATGCTCGCGATCTATCCGGCGTTCGTCACGCTCAACCGCTGGCCCGGCGCGGCTTCGCTGCTGTCGATCAT
CGCGGCGCTCGCGGGCGTGCATGCGATCAACATCGGCGCGACGGGCGCGATGCTCGGCGAGCTGTTCCCGCGCGCGGTGC
GCGCGACGGGCGGCGCGCTCGTCTATAGCGTCGGCGTCGCGATCTTCGGCGGATTCGCGCAATTCTTCGTCACGTGGCTG
ATCGCCGCGACGGGCAATGCGAACGCGCCCGCGTGGTACGCGATCGGCTGCGGCGCGCTGACGCTCGCCGCGCTCGGCTC
GATGGACGACAAGGCGGGCAAGCCGCTCGACTGA

Upstream 100 bases:

>100_bases
ATCCTCGCGATCGGCGCGAGCTATTGGGTGCGGCTTGCCGAAGCATGGCTCGCCGCCTGATGCGGCGCCGCCGCGCCGTC
TCGCGCTCCTCGGGGGTCAC

Downstream 100 bases:

>100_bases
CGCCGCGCGCCGCGGCGCGCGGCCGCGCATCGGCATGGCGGGCGGCTTCGCGAACGATCGCCTCTACGGCGCGGCGCTGC
AATACCAGCGCGGCGGCCTG

Product: transporter protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPFGQLMLAVASFGVGFVTRPLG
GLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQIGIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRG
FYASFQFSSQGLAALAGALTGVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAAAPAPRGDAAPLPLAD
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVTPIGGHLSDRYGRNRIVWWTR
VALMLAIYPAFVTLNRWPGAASLLSIIAALAGVHAINIGATGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNANAPAWYAIGCGALTLAALGSMDDKAGKPLD

Sequences:

>Translated_437_residues
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPFGQLMLAVASFGVGFVTRPLG
GLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQIGIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRG
FYASFQFSSQGLAALAGALTGVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAAAPAPRGDAAPLPLAD
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVTPIGGHLSDRYGRNRIVWWTR
VALMLAIYPAFVTLNRWPGAASLLSIIAALAGVHAINIGATGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNANAPAWYAIGCGALTLAALGSMDDKAGKPLD
>Mature_437_residues
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPFGQLMLAVASFGVGFVTRPLG
GLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQIGIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRG
FYASFQFSSQGLAALAGALTGVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAAAPAPRGDAAPLPLAD
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVTPIGGHLSDRYGRNRIVWWTR
VALMLAIYPAFVTLNRWPGAASLLSIIAALAGVHAINIGATGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNANAPAWYAIGCGALTLAALGSMDDKAGKPLD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=437, Percent_Identity=31.1212814645309, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788942, Length=408, Percent_Identity=32.843137254902, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=30.7328605200946, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789941, Length=417, Percent_Identity=31.6546762589928, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI87082231, Length=236, Percent_Identity=29.2372881355932, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082404, Length=323, Percent_Identity=23.8390092879257, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45378; Mature: 45378

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
GQLMLAVASFGVGFVTRPLGGLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQI
HHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEECCHHHHH
GIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRGFYASFQFSSQGLAALAGALT
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCEEEEEECCCCHHHHHHHHH
GVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAAAPAPRGDAAPLPLAD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVT
HHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PIGGHLSDRYGRNRIVWWTRVALMLAIYPAFVTLNRWPGAASLLSIIAALAGVHAINIGA
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHEEECCC
TGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWLIAATGNANAPAWYAIGCGAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
TLAALGSMDDKAGKPLD
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
GQLMLAVASFGVGFVTRPLGGLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQI
HHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEECCHHHHH
GIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRGFYASFQFSSQGLAALAGALT
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCEEEEEECCCCHHHHHHHHH
GVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAAAPAPRGDAAPLPLAD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVT
HHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PIGGHLSDRYGRNRIVWWTRVALMLAIYPAFVTLNRWPGAASLLSIIAALAGVHAINIGA
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHEEECCC
TGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWLIAATGNANAPAWYAIGCGAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
TLAALGSMDDKAGKPLD
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]