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The map label for this gene is priA [H]

Identifier: 53720997

GI number: 53720997

Start: 4026183

End: 4028450

Strand: Reverse

Name: priA [H]

Synonym: BPSL3390

Alternate gene names: 53720997

Gene position: 4028450-4026183 (Counterclockwise)

Preceding gene: 53720998

Following gene: 53720995

Centisome position: 98.87

GC content: 72.31

Gene sequence:

>2268_bases
ATGGCTGATGCGTTCGTGCGCGTCGTGCTCGATCATCCGCTGCCGACGCTGTTCGATTACCGGTATCGCGGCGACATGCC
GGCGGCGCCCGGGATGCTCGTGCAGGTGCCGTTCGGCCGGCGCAGCGTGGTCGGGCTCGTATGCGAAGTGACCGCTCACA
CGGACGTGCCGGCGGCCAAGCTGAAGGCCGTCGATGCGGTTTGTACGGAGCTGCCGCCGCTGTCGCGGCACTGGCTCGAA
CTCGCCGCGTTCGCGGCCGACTACTATCAGCGCGGGCTCGGTGAAGTTGCGCTGCCGGCGCTGCCGCAGGCGCTGCGCGA
CGTGCCGCGCTGGGGGCGGCTGCTTGCGCCCGAGCTGCGCTACCGGCTGCTCGACGCCGGGCGCGCCGCGCTGCCGGACG
CGCTGCCCGCGCGCGGCGCGGCGCTGCGCCGGCTCGCGCAGGCGCTCGCCGACGCGCCTGCGCTCTCGCTCGCCGAGGTT
CGCGCGCTGCATCCGAAGGCCGTCGCGGTGCTCGACGAATGGCAGGCGCAAGGCTGGGCCGCGTGCGACGAGATTGCGAT
CACGCACGCCGATGCGCAGCTTGTGGATAACCCGGCACCGGCGTCCGCTCGGCGCCCGACTGCATTGCCGCCGCAACTGA
CCGATCAACAGGCGGAGGCGCTCGACGCGATCGCGGCGGCGGACGGTTTCGCGCCATTCCTGCTGCACGGCGTGACGGGC
AGCGGCAAGACCGAGGTGTATCTGCGCGCGCTCGCCGCGCTGCTCGAGCGGCGGCCCGAAGCACAGGCGCTCGTCCTCGT
GCCCGAGATCAACCTGACGCCGCAGTTCGAAGCGGCGTTTCGCGCGCGCTTCGCCGCGACGCTGCCCGCCGATGCGATCG
TCACGCTGCACAGCGGCCTTGCCGAAGGCGAGCGCGCGCGCCACTGGCTCGCCGCACATACCGGCCGCGCGCGGATCGTG
CTCGGCACGCGGCTCGCGGTGCTCGCGTCGCTGCCGGCGCTTGCGATGATCGTCGTCGACGAGGAGCACGAGCCGGCCTA
CAAGCAACAGGAAGGGTTGCGCTATTCGGCGCGCGATCTCGCCGTGTGGCGGGCGAAGCAGCTCGCGATTCCGGTCGTCC
TCGGCTCGGCGACACCGTCGCTCGAAACCTGGTGGCAGGCTGAGCAGGGCCGCTACCGGCGGCTCACGCTGACGAAGCGC
GCGGTGGCCGACGCGGCGCTGCCGACGGTGCGGCTCGTCGATCTCGAGGAGGAGCGGCGGCGCGGGCGCGGGTCGATGGG
CGGGCTGTCGGGGCCGCTCGTCGCTGCGCTGAAGGCGCGGCTCGAGCGTGGCGAGCAGAGCCTCGTGTTCCTGAACCGGC
GCGGCTATGCGCCCGCGCTCGCGTGCGACGCGTGCGGCTGGGTCGCAGGCTGCCCGCGATGCAGCGCGTATGTCGTGATG
CACAAGCCCGAGCACGCGGTGCGCTGCCATCACTGCGGCTGGGAGGCGCGCATTCCGCGCGCGTGCCCGGAATGCGGGAA
CGTCGATATCGCGCCGCTCGGCCGCGGCACGCAGCGCGTCGAGGAGACGCTCGCGCTCGCCGTGCCGGGCGCGCGGATCC
TGCGCATCGACGCGGACAGCACGCGCCGCAAGGGCAGCGCGCAGGCGCTCTTCTCGGACGTGCACGCGGGCGAGGTCGAC
ATCCTCGTCGGCACGCAGATGATCGCGAAGGGGCACGATTTCCAGCGGGTGTCGCTCGTCGGCGTGCTGAATGCGGATAC
CGCATTGTTCTCGCACGATTTTCGCGCAAGCGAGCGGCTATTCGCGCAACTGATGCAGGTGAGCGGCCGCGCGGGGCGCG
CGGGGCTGCCGGGCGAGGTGCTGATCCAGACGCGATATCCGCGTCACGCGCTCTATCACGCGCTCGCGCGCCAGGATTAC
GTGGGGTTCGCCGGTGCGACGCTCGGCGAGCGGCGCGACGCGCACCTGCCGCCGTTCGTCTATCAGGCGCTCTTGCGCGC
CGAGGGGCGCACGCTCGATGCGGCGCTCGCCTTCCTGCAGCAGGCGGCCGACGCGTTCTCGGGGCTGCCGGGCGCGGAGC
GCGTGACGGTGTACGACGCCGTGCCGATGACGATCGTCAAGGTGGCCAATGTCCACCGGGCGCAACTGTTGATCGAGAGC
GCGTCGCGCGCGGCGCTTCAGCACACGCTGCGCGCGTGGCAGCCGCTGCTGCGCGAGCTGAAGGGCGTGCTGCGCTGGAG
CGTCGAGGTCGATCCGCTCGACATCTGA

Upstream 100 bases:

>100_bases
ATCCAAAACTTAGCGCCGAAAGTGACGTTTCACTTTAACTTCACGGGCGTCGCGGATATCCGGCCTGCCGGGCCTGTCGG
CATTCGGCGGTGCCGCACCG

Downstream 100 bases:

>100_bases
CGCCGGCGCGTCATTCGTGCGCTTCGCGCAGGCCGGACAGCCGCGCGCGGCGCTCGTCCTTCTTCACGCGTTGCGCACAT
CCGAGCGCCTGGAAAAACAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MADAFVRVVLDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLE
LAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEV
RALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIV
LGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKR
AVADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVD
ILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDY
VGFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Sequences:

>Translated_755_residues
MADAFVRVVLDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLE
LAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEV
RALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIV
LGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKR
AVADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVD
ILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDY
VGFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
>Mature_754_residues
ADAFVRVVLDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLEL
AAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEVR
ALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTGS
GKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIVL
GTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRA
VADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVMH
KPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVDI
LVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYV
GFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIESA
SRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=759, Percent_Identity=41.3702239789196, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81910; Mature: 81779

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAFVRVVLDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAK
CCCCEEEEEHHCCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHH
LKAVDAVCTELPPLSRHWLELAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCHHHH
YRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVLDEWQAQGWA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
ACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
CHHHHEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCC
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGL
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
AEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDL
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCHHHH
AVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVRLVDLEEERR
HHHHHHHEEEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEECCHHHHH
RGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCCCCCCEEEEE
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADS
ECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
TRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERL
CCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHH
FAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGERRDAHLPPFV
HHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure 
ADAFVRVVLDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAK
CCCEEEEEHHCCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHH
LKAVDAVCTELPPLSRHWLELAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCHHHH
YRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVLDEWQAQGWA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
ACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
CHHHHEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCC
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGL
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
AEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDL
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCHHHH
AVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVRLVDLEEERR
HHHHHHHEEEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEECCHHHHH
RGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCCCCCCEEEEE
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADS
ECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
TRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERL
CCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHH
FAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGERRDAHLPPFV
HHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]