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The map label for this gene is eutR [H]

Identifier: 53720977

GI number: 53720977

Start: 3996544

End: 3997623

Strand: Reverse

Name: eutR [H]

Synonym: BPSL3368

Alternate gene names: 53720977

Gene position: 3997623-3996544 (Counterclockwise)

Preceding gene: 53720979

Following gene: 53720974

Centisome position: 98.11

GC content: 72.87

Gene sequence:

>1080_bases
TTGCGTAATCGGGCGGACCGTTTCAGCTTCAGCGACGCACAAGGAGGCGCGATGGATCACGAGCACACCGGCGCCGAGAC
GACGGCGGAGGACACCGGGCGCAGCGCGGACGGCGACGGCGGCGCGGCCGGGCGCGCGCTCGTGAGCGTCGCGCACGACG
CCGACGAGCAGGCGCGCAACCTGATCGGCTGGCGCCAGACCTACGACCAGCTCGCGGCGGGCCGCTTCGTCGGCACGTTG
ACCGAGCTGCCGCTCGACACGATGAAGGTGTTCCGGGAGACGACGAGCCATACGCTGCGGCAGGCGTGCGAGGTGCGCGG
CGATGCGTACTGGTTCGGCATTCCGCTCGCGCGCGACGGCGCGGCGCGCATCGACGCGCGGCCGATCGCCGCCGACGCGC
TCGCGTTCCGGCCCGGCAACGTCGAGTTCGAGCTGTTGACGCCCGCGCAATTCTCGATCTACGGGGTGGTCGTGCGCGGC
GCGGTGTTGCGCCGTTACGCGCAGGAGGTCGAGCGCTGCGGGCTCGACGAGCGGTTGCCGCTCGTGCCCGTCGTGCGCGT
CGGCGAGGCGCGGCTCACGCGGCTGTGCGCGTTGCTCGCGCAGCGTCTGGACGACGCCGACGCGATGAGCGCGGCGGGCG
AGCCGCTATCCGACTGCGCGCGCAACGACCTGCAGGCGGAAGTGCTCGCGGCGCTGTTCGACCTGTGCGCGTCGCCCGCG
GCCGACGCGAGCGTCGAGCACTCGTCGCGGCGCCGCAAGATCGTCGCGGCCGCGCGCGACTACGTGCTCGCGCATCGCTC
GCGGCCTGTCGGCGTGCCGGAGCTGTGCGAGCAACTGCACGTGAGCCGGCGCACGCTGCAGTATTGCTTCCAGGATGTGC
TCGGGATGGCGCCCGCGACCTACCTGCGCGCGCTGCGGCTTAACGGCGTGCGGCGCGATCTGCGCGGCCGCGCGGCCGCC
TCGGTGCAGGACGCCGCGGCTGCATGGGGGTTTTGGCATCTGAGCCAGTTCGCGACCGATTATCGGCGGATGTTCGGCGC
GCGGCCGTCGGAGACGCTGCGCGACGCGCTCGCCTGTTGA

Upstream 100 bases:

>100_bases
GCGCCATTGCTGAACACTCGCCGCGCGCCGGTTGTTCAAATGCGGCACAGCGTCGCATCGACACCGGCGATTTTGGCGAC
GGTACGCTTCGGCATGGGAA

Downstream 100 bases:

>100_bases
GGCGGCGAGAAGGAGGCGAAGGGCGCGGCGCACCGGCCGCCGGCGTTCACCGGCCAGCCGGCGACGCTGCGGCGGCGGCC
GCATACCGCGCGAGACCGCC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Ethanolamine operon regulatory protein [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MRNRADRFSFSDAQGGAMDHEHTGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFVGTL
TELPLDTMKVFRETTSHTLRQACEVRGDAYWFGIPLARDGAARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRG
AVLRRYAQEVERCGLDERLPLVPVVRVGEARLTRLCALLAQRLDDADAMSAAGEPLSDCARNDLQAEVLAALFDLCASPA
ADASVEHSSRRRKIVAAARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGRAAA
SVQDAAAAWGFWHLSQFATDYRRMFGARPSETLRDALAC

Sequences:

>Translated_359_residues
MRNRADRFSFSDAQGGAMDHEHTGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFVGTL
TELPLDTMKVFRETTSHTLRQACEVRGDAYWFGIPLARDGAARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRG
AVLRRYAQEVERCGLDERLPLVPVVRVGEARLTRLCALLAQRLDDADAMSAAGEPLSDCARNDLQAEVLAALFDLCASPA
ADASVEHSSRRRKIVAAARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGRAAA
SVQDAAAAWGFWHLSQFATDYRRMFGARPSETLRDALAC
>Mature_359_residues
MRNRADRFSFSDAQGGAMDHEHTGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFVGTL
TELPLDTMKVFRETTSHTLRQACEVRGDAYWFGIPLARDGAARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRG
AVLRRYAQEVERCGLDERLPLVPVVRVGEARLTRLCALLAQRLDDADAMSAAGEPLSDCARNDLQAEVLAALFDLCASPA
ADASVEHSSRRRKIVAAARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGRAAA
SVQDAAAAWGFWHLSQFATDYRRMFGARPSETLRDALAC

Specific function: Activates the transcription of the eut operon. Also positively regulates its own transcription. Probably binds ethanolamine and vitamin B12 as effectors [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=311, Percent_Identity=38.5852090032154, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 39258; Mature: 39258

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRADRFSFSDAQGGAMDHEHTGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIGWRQTYDQLAAGRFVGTLTELPLDTMKVFRETTSHTLRQACEVRGDAYWFGIPLARDG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCC
AARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRGAVLRRYAQEVERCGLDERLP
CCCCCCCCCHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVPVVRVGEARLTRLCALLAQRLDDADAMSAAGEPLSDCARNDLQAEVLAALFDLCASPA
CCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCC
ADASVEHSSRRRKIVAAARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPAT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YLRALRLNGVRRDLRGRAAASVQDAAAAWGFWHLSQFATDYRRMFGARPSETLRDALAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MRNRADRFSFSDAQGGAMDHEHTGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIGWRQTYDQLAAGRFVGTLTELPLDTMKVFRETTSHTLRQACEVRGDAYWFGIPLARDG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCC
AARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRGAVLRRYAQEVERCGLDERLP
CCCCCCCCCHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVPVVRVGEARLTRLCALLAQRLDDADAMSAAGEPLSDCARNDLQAEVLAALFDLCASPA
CCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCC
ADASVEHSSRRRKIVAAARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPAT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YLRALRLNGVRRDLRGRAAASVQDAAAAWGFWHLSQFATDYRRMFGARPSETLRDALAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503 [H]