Click here to switch to the map view.

The map label for this gene is cheB

Identifier: 53720911

GI number: 53720911

Start: 3919557

End: 3920660

Strand: Reverse

Name: cheB

Synonym: BPSL3301

Alternate gene names: 53720911

Gene position: 3920660-3919557 (Counterclockwise)

Preceding gene: 53720912

Following gene: 53720910

Centisome position: 96.22

GC content: 68.39

Gene sequence:

>1104_bases
TTGAACGCTGTGCAGAAGAAAATCAAAGTGCTGTGCGTCGACGATTCGGCGCTGATCCGCAGCCTGATGACAGAAATCAT
CAACAGCCAGCCCGACATGGAAGTGTGCGCAACGGCGCCCGATCCGCTCGTGGCGCGCGAGCTGATCAAGCAGCACAACC
CGGACGTGCTGACGCTCGACGTCGAAATGCCGCGGATGGACGGGCTCGACTTCCTTGAAAAGCTGATGCGCCTGCGGCCG
ATGCCGGTCGTGATGGTGTCGTCGCTCACCGAGCGCGGCTCGGAGATCACGCTGCGCGCGCTCGAGCTGGGCGCGGTCGA
TTTCGTGACGAAGCCGCGCGTCGGGATTCGCGACGGCATGCTCGACTATTCGGAAAAGCTGGCCGACAAGGTGCGCGCGG
CATCGCGCGCGCGCGTGCGGCAGAATCCGCAGCCGCATGCGGCCGCCGCCGCGGCCGCGCACGGCCACGCGGGCGCCGCC
GCGCCGCTCATCAACAATCCGCTTGTCAGTACCGAGAAGCTGATCATCGTCGGCGCGTCCACGGGCGGCACCGAGGCGAT
CCGCGAAGTGCTGACGCCGCTGCCGCCCGATGCGCCCGCCGTGCTGATCGCGCAGCACATGCCGCCCGGCTTCACGCGCT
CGTTCGCGCAGCGCCTGAACGGGCTGTGCCGGATCTCGGTGAAGGAGGCGGAGCACGGCGAGCGCGTGCTGCCGGGGCAC
GCGTATATCGCGCCCGGCCACGCGCACCTGCTGCTCGCGAGGAGCGGCGCGAACTACATCGCGCACCTGTCGGACGAGCC
GCCCGTCAATCGTCATCGCCCGTCGGTCGACGTGCTGTTCCGCTCGGCCGCGCAGCATGCGGGCAAGAACGCGCTCGGCG
TGATCCTGACGGGCATGGGCCGCGACGGTGCGGCCGGGCTGCTTGAAATGAAAAAGGCCGGCGCGTATACATTTGCGCAG
GACGAGGCGAGCTGCGTCGTGTTCGGAATGCCGCGCGAGGCGATCGCGATGGGCGGCGTCGACGACGTCGCGCCCCTTTC
CGACATGAGTCGCCGTATCATGGCGCGCCTCGCGTCGATGGGCGATCGCGTGCAGCGCGTGTGA

Upstream 100 bases:

>100_bases
TCGAGCTGTTCGGCACGCCCGCGCCGAAGGCGCAGGCGAAGCCTCGCATCGAATTGTTCGGTATGCGGGCCATGCAGCCC
GCCACAAGAAAGCAGGAGGC

Downstream 100 bases:

>100_bases
CGCCCGGCCCGGTTCGCGACGACGACGTGCTTGCGCCGGCCGCCGCAAGGGCCGCACGCGAGGAATGGCACACCGCGAAT
GCTGACGTATTCGCGACGAG

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNAVQKKIKVLCVDDSALIRSLMTEIINSQPDMEVCATAPDPLVARELIKQHNPDVLTLDVEMPRMDGLDFLEKLMRLRP
MPVVMVSSLTERGSEITLRALELGAVDFVTKPRVGIRDGMLDYSEKLADKVRAASRARVRQNPQPHAAAAAAAHGHAGAA
APLINNPLVSTEKLIIVGASTGGTEAIREVLTPLPPDAPAVLIAQHMPPGFTRSFAQRLNGLCRISVKEAEHGERVLPGH
AYIAPGHAHLLLARSGANYIAHLSDEPPVNRHRPSVDVLFRSAAQHAGKNALGVILTGMGRDGAAGLLEMKKAGAYTFAQ
DEASCVVFGMPREAIAMGGVDDVAPLSDMSRRIMARLASMGDRVQRV

Sequences:

>Translated_367_residues
MNAVQKKIKVLCVDDSALIRSLMTEIINSQPDMEVCATAPDPLVARELIKQHNPDVLTLDVEMPRMDGLDFLEKLMRLRP
MPVVMVSSLTERGSEITLRALELGAVDFVTKPRVGIRDGMLDYSEKLADKVRAASRARVRQNPQPHAAAAAAAHGHAGAA
APLINNPLVSTEKLIIVGASTGGTEAIREVLTPLPPDAPAVLIAQHMPPGFTRSFAQRLNGLCRISVKEAEHGERVLPGH
AYIAPGHAHLLLARSGANYIAHLSDEPPVNRHRPSVDVLFRSAAQHAGKNALGVILTGMGRDGAAGLLEMKKAGAYTFAQ
DEASCVVFGMPREAIAMGGVDDVAPLSDMSRRIMARLASMGDRVQRV
>Mature_367_residues
MNAVQKKIKVLCVDDSALIRSLMTEIINSQPDMEVCATAPDPLVARELIKQHNPDVLTLDVEMPRMDGLDFLEKLMRLRP
MPVVMVSSLTERGSEITLRALELGAVDFVTKPRVGIRDGMLDYSEKLADKVRAASRARVRQNPQPHAAAAAAAHGHAGAA
APLINNPLVSTEKLIIVGASTGGTEAIREVLTPLPPDAPAVLIAQHMPPGFTRSFAQRLNGLCRISVKEAEHGERVLPGH
AYIAPGHAHLLLARSGANYIAHLSDEPPVNRHRPSVDVLFRSAAQHAGKNALGVILTGMGRDGAAGLLEMKKAGAYTFAQ
DEASCVVFGMPREAIAMGGVDDVAPLSDMSRRIMARLASMGDRVQRV

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=353, Percent_Identity=69.4050991501417, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_BURPS (Q63PS2)

Other databases:

- EMBL:   BX571965
- RefSeq:   YP_109897.1
- ProteinModelPortal:   Q63PS2
- SMR:   Q63PS2
- GeneID:   3092815
- GenomeReviews:   BX571965_GR
- KEGG:   bps:BPSL3301
- HOGENOM:   HBG705324
- OMA:   CAGANTT
- ProtClustDB:   PRK00742
- BioCyc:   BPSE272560:BPSL3301-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39353; Mature: 39353

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY; PS00936 RIBOSOMAL_L35

Important sites: ACT_SITE 180-180 ACT_SITE 206-206 ACT_SITE 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVQKKIKVLCVDDSALIRSLMTEIINSQPDMEVCATAPDPLVARELIKQHNPDVLTLD
CCCHHCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEE
VEMPRMDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAVDFVTKPRVGIRDGM
ECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHCH
LDYSEKLADKVRAASRARVRQNPQPHAAAAAAAHGHAGAAAPLINNPLVSTEKLIIVGAS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECC
TGGTEAIREVLTPLPPDAPAVLIAQHMPPGFTRSFAQRLNGLCRISVKEAEHGERVLPGH
CCCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCEECCCC
AYIAPGHAHLLLARSGANYIAHLSDEPPVNRHRPSVDVLFRSAAQHAGKNALGVILTGMG
EEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCC
RDGAAGLLEMKKAGAYTFAQDEASCVVFGMPREAIAMGGVDDVAPLSDMSRRIMARLASM
CCCCHHHHHHHHCCCEEEECCCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GDRVQRV
HHHHHCC
>Mature Secondary Structure
MNAVQKKIKVLCVDDSALIRSLMTEIINSQPDMEVCATAPDPLVARELIKQHNPDVLTLD
CCCHHCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEE
VEMPRMDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAVDFVTKPRVGIRDGM
ECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHCH
LDYSEKLADKVRAASRARVRQNPQPHAAAAAAAHGHAGAAAPLINNPLVSTEKLIIVGAS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECC
TGGTEAIREVLTPLPPDAPAVLIAQHMPPGFTRSFAQRLNGLCRISVKEAEHGERVLPGH
CCCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCEECCCC
AYIAPGHAHLLLARSGANYIAHLSDEPPVNRHRPSVDVLFRSAAQHAGKNALGVILTGMG
EEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCC
RDGAAGLLEMKKAGAYTFAQDEASCVVFGMPREAIAMGGVDDVAPLSDMSRRIMARLASM
CCCCHHHHHHHHCCCEEEECCCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GDRVQRV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA