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The map label for this gene is fliA [H]

Identifier: 53720901

GI number: 53720901

Start: 3908047

End: 3908778

Strand: Reverse

Name: fliA [H]

Synonym: BPSL3291

Alternate gene names: 53720901

Gene position: 3908778-3908047 (Counterclockwise)

Preceding gene: 53720902

Following gene: 53720900

Centisome position: 95.93

GC content: 66.94

Gene sequence:

>732_bases
ATGTATAACGCTCAAGGCAAGATTTCCCAGGACGAAGTGCTGACGCAATACGCGCCGCTCGTGCGTCGTCTCGGCCTGCA
GCTCGTCGCGAAGATGCCGGCGAGCGTGGACCTCGACGATCTGATCCAGGCGGGCATGATCGGCCTGCTCGACGCGGCGA
GCCGCTACAAGGAAGATCAGGGCGCGCAGTTCGAGACCTACGCGACGCAGCGCATCCGCGGCGCGATGCTCGACGAGCTG
CGCAGCAACGATTGGCTGCCGCGCAGCCTGCGCAAGACCTCGCGCGAAGTCGAACATGCGGTGCATCAGGTCGAGCAGCG
GCTCGGCCGCTCGGCGAACGAGACGGAGATCGCCGCGCACCTGCAGATGCCGCTCGACGAATACCAGTCGATGCTGCAGG
ATCTGCACGGCAGCCAGCTCGTCTACTACGAGGACTTCGACCGCTCGGCCGACGACGAGCCGTTTCTCGACCGCTACCGC
GCCGATCACAGCGATCCGCTGTCGGCGCTGCTCGACGAGCATCTGCGCAGCGCGCTCGTCGATGCGATCGAGCGCCTGCC
GGAGCGCGAGAAGCTGCTGATGTCGCTGTACTACGAGCGCGGGCTGAACCTGCGCGAGATCGGCGCGGTGCTCGAGGTGA
GCGAGTCGCGCGTGTGCCAGTTGCATAGCCAGGCGGTCGCGCGGCTGCGCGCGAAGCTGCGCGAGCAGGCGTGGGTCGGC
ACGGAGAGCTGA

Upstream 100 bases:

>100_bases
GCGCCCGGGTCCGGGCATCGGCCGGGTCCGGACGGGAAGTTCGGCGTTTGAACGCGGCGCGGCTCATGCCGCCTGAACGC
CACGACGAAGAGAGGGCATG

Downstream 100 bases:

>100_bases
CGCGGCCGCGCGGCGCCCGGGCGCGCGAAGCGCGGCTTGAAATGCGGGCCCGAAGGCGCGCCGGCGACGCGGGCGGAATG
CCGTCGGAGCGCGGCACGGA

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYATQRIRGAMLDEL
RSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAHLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRYR
ADHSDPLSALLDEHLRSALVDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWVG
TES

Sequences:

>Translated_243_residues
MYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYATQRIRGAMLDEL
RSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAHLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRYR
ADHSDPLSALLDEHLRSALVDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWVG
TES
>Mature_243_residues
MYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYATQRIRGAMLDEL
RSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAHLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRYR
ADHSDPLSALLDEHLRSALVDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWVG
TES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=233, Percent_Identity=53.6480686695279, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1789448, Length=205, Percent_Identity=28.2926829268293, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27797; Mature: 27797

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAQFETYATQRIRGAMLDELRSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAH
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRYRADHSDPLSALLDEHLRSALV
HHCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
DAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWVG
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TES
CCC
>Mature Secondary Structure
MYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAQFETYATQRIRGAMLDELRSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAH
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRYRADHSDPLSALLDEHLRSALV
HHCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
DAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWVG
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TES
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]