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The map label for this gene is rhtB [H]

Identifier: 53720860

GI number: 53720860

Start: 3864817

End: 3865575

Strand: Reverse

Name: rhtB [H]

Synonym: BPSL3252

Alternate gene names: 53720860

Gene position: 3865575-3864817 (Counterclockwise)

Preceding gene: 53720861

Following gene: 53720858

Centisome position: 94.87

GC content: 68.12

Gene sequence:

>759_bases
GTGCATCGTGCCGCCGTCCCGCCGCAGACAGCGACACGCATTCCCCGGCATAATCGGCGTTTCCATTTCATTTACGACAG
CGGGGCGGCAATGAATCTGCATACGTGGTGGCTGTTCGTCGCGACGGTGTTCGTCGTGTCGGCGATTCCCGGGCCGAACA
TGTTGCTCGTGATGACGCACGGTGCGCGTCACGGGCTACGGCGTTCGACGGCGACGATGGCGGGCTGCATGACGGCGCTC
GTCGCGATGCTGTCGGTGTCCGCCGCGGGCCTCGGCGTGTTCCTCGAGGCGTGGCCCGCGATGTTCGATGCGCTGCGTTT
CGCGGGCGCCGCGTATCTGATCTATCTGGGCGTGAAGGCATGGCGCGCGCGCGTCGACGAGCCGTCCGATGCGCAATCCA
ATGCGCACGCCGAGGCCGCGGCGCCGCGCGCGGGCCGTTCGGGCTCGGCGGGCCAGGGGTCTCGTTGGGCGCTCTTTCGC
AACGGCTTTCTCGTCGCGGGCAGCAATCCGAAGGCGATCCTGTTCGCCGCCGCGCTGCTGCCGCAGTTCATCGACGCTTC
CGCGCCGACGCTGCCGCAGTTCGGCATCCTCGTCGCGACGTTCGCGGTGATCGAGGTGAGCTGGTACATCGTCTACGCGT
CGTTCGGCACGCGGATCGGCGTGACGCTGCGCAGCGCGAACGTCGCGAAGGCGTTCAATCGGCTGACGGGCGGCCTGTTC
GTCGGCTTCGGCGCGATGATGGCGCTCGTGCGGCACTGA

Upstream 100 bases:

>100_bases
GCGATAGCCTGATCGAAGCGGTCGCGCACGGCCACGGCGACAAGGATTTCGCGGTGCTCGGCCAGGTCGCGGCGCGCCGC
GCGGGGCGCTGACGCGCGCG

Downstream 100 bases:

>100_bases
AACACGCGCGCGGCAGGCTCGGCGCGCCCCGCCGCGCCGCATGAACGAATGAAACGCCCCGCGTCGGTGACGGCGCGGGG
CGTCGTCGTTTCAGCGGGGC

Product: putative amino acid transport protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MHRAAVPPQTATRIPRHNRRFHFIYDSGAAMNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTAL
VAMLSVSAAGLGVFLEAWPAMFDALRFAGAAYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFR
NGFLVAGSNPKAILFAAALLPQFIDASAPTLPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLF
VGFGAMMALVRH

Sequences:

>Translated_252_residues
MHRAAVPPQTATRIPRHNRRFHFIYDSGAAMNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTAL
VAMLSVSAAGLGVFLEAWPAMFDALRFAGAAYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFR
NGFLVAGSNPKAILFAAALLPQFIDASAPTLPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLF
VGFGAMMALVRH
>Mature_252_residues
MHRAAVPPQTATRIPRHNRRFHFIYDSGAAMNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTAL
VAMLSVSAAGLGVFLEAWPAMFDALRFAGAAYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFR
NGFLVAGSNPKAILFAAALLPQFIDASAPTLPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLF
VGFGAMMALVRH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=224, Percent_Identity=33.9285714285714, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788099, Length=223, Percent_Identity=30.4932735426009, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 26991; Mature: 26991

Theoretical pI: Translated: 11.83; Mature: 11.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRAAVPPQTATRIPRHNRRFHFIYDSGAAMNLHTWWLFVATVFVVSAIPGPNMLLVMTH
CCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEC
GARHGLRRSTATMAGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGAAYLIYLGVKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFRNGFLVAGSNPKAILFAAALL
HHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHHHH
PQFIDASAPTLPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLF
HHHHCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHH
VGFGAMMALVRH
HHHHHHHHHHCC
>Mature Secondary Structure
MHRAAVPPQTATRIPRHNRRFHFIYDSGAAMNLHTWWLFVATVFVVSAIPGPNMLLVMTH
CCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEC
GARHGLRRSTATMAGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGAAYLIYLGVKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFRNGFLVAGSNPKAILFAAALL
HHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHHHH
PQFIDASAPTLPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLF
HHHHCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHH
VGFGAMMALVRH
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]