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The map label for this gene is trmJ [H]

Identifier: 53719858

GI number: 53719858

Start: 2711247

End: 2712158

Strand: Reverse

Name: trmJ [H]

Synonym: BPSL2249

Alternate gene names: 53719858

Gene position: 2712158-2711247 (Counterclockwise)

Preceding gene: 53719860

Following gene: 53719857

Centisome position: 66.56

GC content: 71.27

Gene sequence:

>912_bases
TTGGAATCTCAGCAGAAACCGTCCGCGCCGCCTTCCGGCGCGGCCACATCCCGTCCGGCGCGGACCGCGCGCGGCGGATT
CACGTCGACGCGCTTCGTGCTCGTCGAGCCGAGCCACCCCGGCAACGTCGGCGCGGCGGCGCGCGCGCTGAAGACGATGG
GTTTTTCCCGCCTCGTGCTCGTCGCGCCGCGCGTGCCGCGCGTGCAGAGCGATCCGGAGGCGCTCGCGATGGCGAGCGGC
GCCGACGATGTGCTCGCGTCCGCGCACGTCGTGCCGACGCTCGCCGACGCGCTGAACGGCGTGCAGTGGTCGCTCGCGCT
CACCGCGCGTTCGCGCGAGTACGGCCCGCCGCGGCTCGCGCCCCGCGCGGCCGTCGCGAGCGCGCTTCAGCAGGTGCGCA
CGGGCGATATCGCGCTCGTGTTCGGCAACGAACGCACCGGCCTGTCGAACGAGCACGTCGAGCGCTGCAGCGCGATCGCG
CACATTCCGGCGAACCCCGCATACAGTTCGCTCAACCTCGCGCAGGCGGTGCAGGTGCTCGCGTACGAGCTGCGCGTCGC
GCTGCTCGAAGACGACGAGGCGGCGGTGCCGCCGGACGCCGCGCTCGGCACGCTCGCGCAAAGCGACGAGATCGAGCGGA
TGTTCGTCCATCTGGAAAATGCGCTGATCGCGCTCGATTTCCTCGATCCGCGCAATCCGAAGAAGCTGATGCCGCGGCTG
CGGCGGCTCTTCGCGCGAACGGGGCTCGAACGCGAAGAAGTGAACATCGTTCGAGGCATCGCGAAGCACATCCTGCTGAA
GGCGGGCGGGGCGAACGCGCGCGCGGGCGCTTCGGGCGAGGGCAACGGCAACGGCGAAAGCGCCACCGGCGACGCGGACT
ATTCGGGCCGCGACGTCAGCCGAGACGACTGA

Upstream 100 bases:

>100_bases
AAACGGTTGCGCGGGCCGTTCGATCGGCCGTTCGGACGAGTGGGTTTGTCGGCGAGTTGGCGATACCATACCGGCATCCC
GATCAGTCGAAGTGAACGTT

Downstream 100 bases:

>100_bases
CGCCGCGCGGTCTCGCGGTCGTGCCATACCCGTGATCGCGGCAGGGGGAACGCGGCGCCTGCTGCGCGTGCGCGGCGGCG
GCTCTACAATCGTCCGCATG

Product: putative tRNA/rRNA methyltransferase protein

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPRVQSDPEALAMASG
ADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAVASALQQVRTGDIALVFGNERTGLSNEHVERCSAIA
HIPANPAYSSLNLAQAVQVLAYELRVALLEDDEAAVPPDAALGTLAQSDEIERMFVHLENALIALDFLDPRNPKKLMPRL
RRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNGESATGDADYSGRDVSRDD

Sequences:

>Translated_303_residues
MESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPRVQSDPEALAMASG
ADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAVASALQQVRTGDIALVFGNERTGLSNEHVERCSAIA
HIPANPAYSSLNLAQAVQVLAYELRVALLEDDEAAVPPDAALGTLAQSDEIERMFVHLENALIALDFLDPRNPKKLMPRL
RRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNGESATGDADYSGRDVSRDD
>Mature_303_residues
MESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPRVQSDPEALAMASG
ADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAVASALQQVRTGDIALVFGNERTGLSNEHVERCSAIA
HIPANPAYSSLNLAQAVQVLAYELRVALLEDDEAAVPPDAALGTLAQSDEIERMFVHLENALIALDFLDPRNPKKLMPRL
RRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNGESATGDADYSGRDVSRDD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=247, Percent_Identity=43.3198380566802, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=39.2405063291139, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31944; Mature: 31944

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVL
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEE
VAPRVPRVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLA
ECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
PRAAVASALQQVRTGDIALVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AYELRVALLEDDEAAVPPDAALGTLAQSDEIERMFVHLENALIALDFLDPRNPKKLMPRL
HHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
RRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNGESATGDADYSGRDVS
HHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RDD
CCC
>Mature Secondary Structure
MESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVL
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEE
VAPRVPRVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLA
ECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
PRAAVASALQQVRTGDIALVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AYELRVALLEDDEAAVPPDAALGTLAQSDEIERMFVHLENALIALDFLDPRNPKKLMPRL
HHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
RRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNGESATGDADYSGRDVS
HHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RDD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA