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The map label for this gene is gltX [H]

Identifier: 53719806

GI number: 53719806

Start: 2637135

End: 2638544

Strand: Reverse

Name: gltX [H]

Synonym: BPSL2197

Alternate gene names: 53719806

Gene position: 2638544-2637135 (Counterclockwise)

Preceding gene: 53719808

Following gene: 53719805

Centisome position: 64.76

GC content: 67.02

Gene sequence:

>1410_bases
ATGACCCGTCCTGTACGCACCCGTTTTGCGCCGAGCCCCACCGGCTTCATCCATCTCGGCAACATCCGATCCGCGCTCTA
TCCGTGGGCGTTCGCCCGCAAGATGAAGGGCACGTTCGTGCTGCGGATCGAGGATACGGATCTCGAGCGCTCGACCGAGG
CGTCGGTCGATGCGATCCTCGAAGGGATGGCATGGCTTGGGCTCGACTACGACGAAGGCCCGTACTACCAGATGCAGCGG
ATGGACCGCTATCGCGAGGTGCTCGCGCAGATGCTCGAGAAAGACCTCGTCTACCCGTGCTACATGTCGACCGAAGAACT
CGATGCGTTGCGCGAGCGCCAGCGCGCGGCGGGCGAGAAGCCGCGCTACGATGGCACGTGGCGCCCCGAGCCGGGCAAGG
TGCTGCCCGAGCCGCCGGCGGGCGTGACCCCCGTGCTGCGCTTTCGCAATCCGCTGACGGGGTCGGTCGTCTGGGACGAC
GCCGTGAAGGGCCGCGTCGAGATCTCGAACGAGGAGCTCGACGATCTCGTCATCGCGCGCCCCGACGGCACGCCGACGTA
CAACTTCTGCGTCGTCGTCGACGATCTCGACATGGGCATCACGCACGTGATCCGCGGGGACGACCATGTGAACAATACGC
CGCGCCAGATCAACATCCTGCGCGCGCTCGGCGGCGAAGTGCCGGTGTACGCGCATCTGCCGACCGTGCTCAACGAGCAG
GGCGAGAAGATGAGCAAGCGCCACGGCGCGATGAGCGTGATGGGCTACCGCGATGCCGGCTATCTGCCGGAGGCGGTGCT
CAACTATCTCGCGCGTCTCGGCTGGTCGCACGGCGACGCGGAGATCTTCTCGCGCGAGCAGTTCGTCGAATGGTTCGATC
TCGAGCATCTCGGCAAGTCGCCCGCGCAGTACGATCACAACAAGCTGAACTGGCTGAACAACCACTACATCAAGGAAGCG
GACGACGCGCGCCTGGCCGAGCTGGCAAAGCCGTTCTTCGCGGCGCTCGGCATCGACGCCGACACGATCGCGCGGGGCCC
GGACCTCGTGGGCGTGATGGGGCTGATGAAGGATCGCGCGTCGACGGTGAAGGAGATCGCGGAGAATTCGACGATGTTCT
ACCGTGCGCCCGCCCCGGATGCGCAAGCGCTCGCGCAGCACGTGACCGACGCGGTGCGCCCGGCGCTCGCCGAGTTTGCC
GCGGCGCTGAAGACGGCCGAGTGGACGAAGGAGGCGATCGCGGCCGCGCTGAAGGCCGTGCTCGGCGCGCACAAGCTGAA
GATGCCGCAGCTTGCGATGCCGGTGCGCCTGCTCGTCGCGGGCACGACGCATACGCCGTCGATCGACGCGGTGCTGCTGC
TGTTCGGCCGCGACGTCGTCGTGTCGCGCCTCGCGGCGGCGCTCGCATAG

Upstream 100 bases:

>100_bases
CTTTCCGTTCCGGTCGCGTGCGCACCCGGCAGAGCCGTGCGGCGTGCGCCGCCGCGTCGTTTCAATTGATGCGCCGCTTG
AGGCGTTCGAGCTATCGTTT

Downstream 100 bases:

>100_bases
GCGCGCGGGGCGTCCGGGAAGCCGTGCGGTTGAGGCAACGAACCGAGGCAACGCACGCGTCGAATGCACGGCTTCCAAAA
ATTTTGCGCGAACCGCAAAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDLERSTEASVDAILEGMAWLGLDYDEGPYYQMQR
MDRYREVLAQMLEKDLVYPCYMSTEELDALRERQRAAGEKPRYDGTWRPEPGKVLPEPPAGVTPVLRFRNPLTGSVVWDD
AVKGRVEISNEELDDLVIARPDGTPTYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEA
DDARLAELAKPFFAALGIDADTIARGPDLVGVMGLMKDRASTVKEIAENSTMFYRAPAPDAQALAQHVTDAVRPALAEFA
AALKTAEWTKEAIAAALKAVLGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRLAAALA

Sequences:

>Translated_469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDLERSTEASVDAILEGMAWLGLDYDEGPYYQMQR
MDRYREVLAQMLEKDLVYPCYMSTEELDALRERQRAAGEKPRYDGTWRPEPGKVLPEPPAGVTPVLRFRNPLTGSVVWDD
AVKGRVEISNEELDDLVIARPDGTPTYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEA
DDARLAELAKPFFAALGIDADTIARGPDLVGVMGLMKDRASTVKEIAENSTMFYRAPAPDAQALAQHVTDAVRPALAEFA
AALKTAEWTKEAIAAALKAVLGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRLAAALA
>Mature_468_residues
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDLERSTEASVDAILEGMAWLGLDYDEGPYYQMQRM
DRYREVLAQMLEKDLVYPCYMSTEELDALRERQRAAGEKPRYDGTWRPEPGKVLPEPPAGVTPVLRFRNPLTGSVVWDDA
VKGRVEISNEELDDLVIARPDGTPTYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQG
EKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEAD
DARLAELAKPFFAALGIDADTIARGPDLVGVMGLMKDRASTVKEIAENSTMFYRAPAPDAQALAQHVTDAVRPALAEFAA
ALKTAEWTKEAIAAALKAVLGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRLAAALA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=330, Percent_Identity=35.1515151515151, Blast_Score=196, Evalue=5e-50,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=49.5708154506438, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=37.1428571428571, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=474, Percent_Identity=28.9029535864979, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324540, Length=362, Percent_Identity=35.0828729281768, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=26.6094420600858, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=28.9156626506024, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52177; Mature: 52046

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDLERSTEASVDAIL
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMAWLGLDYDEGPYYQMQRMDRYREVLAQMLEKDLVYPCYMSTEELDALRERQRAAGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPAGVTPVLRFRNPLTGSVVWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNNHYIKEADDARLAELAKPFFAALGIDADTIARGPDLVGVMGLMKDRA
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHH
STVKEIAENSTMFYRAPAPDAQALAQHVTDAVRPALAEFAAALKTAEWTKEAIAAALKAV
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRLAAALA
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDLERSTEASVDAIL
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMAWLGLDYDEGPYYQMQRMDRYREVLAQMLEKDLVYPCYMSTEELDALRERQRAAGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPAGVTPVLRFRNPLTGSVVWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNNHYIKEADDARLAELAKPFFAALGIDADTIARGPDLVGVMGLMKDRA
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHH
STVKEIAENSTMFYRAPAPDAQALAQHVTDAVRPALAEFAAALKTAEWTKEAIAAALKAV
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRLAAALA
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA