Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 53719772

GI number: 53719772

Start: 2596181

End: 2596714

Strand: Reverse

Name: def [H]

Synonym: BPSL2163

Alternate gene names: 53719772

Gene position: 2596714-2596181 (Counterclockwise)

Preceding gene: 53719773

Following gene: 53719768

Centisome position: 63.73

GC content: 66.67

Gene sequence:

>534_bases
ATGATTCGCGAGATTTTGAAGATGGGCGATCCACGTCTGCTGGAAGTCGCCCGGCCGGTGGAGGCGTTCAACACGCCGGA
GCTGCACGCGCTCGTCGCGGACATGTTCGAGACGATGCATCACGCGAACGGCGCGGGGCTCGCCGCGCCGCAGATCGGCG
TCGGGTTGCAGGTGATCATCTTCGGCTTCGGCAGCAGCGAGCGCTACCCGGAGGCGCCGCCCGTGCCGGAGACGGTGCTC
GTCAATCCGAGCATCGAGTACTTGCCGCCCGACCTGGAGGAGGGCTGGGAGGGCTGCCTGTCGGTGCCGGGGCTGCGCGG
CGTCGTGAGCCGCTACCGGCGCGTGCGCTACAGCGGCTTCGACCAGTACGGCGCGAAGCTCGAGCGGATCGCGGAAGGCT
TCCACGCGCGGGTCGTCCAGCACGAATACGATCATCTGATCGGCAAGCTCTATCCGATGCGGATCACCGATTTTTCGAAG
TTCGGCTTCGCCGACGTGCTGTTCCCGGGGCTCGACGCGCAAGCGGACGACTGA

Upstream 100 bases:

>100_bases
CGGGCGCCGACGCGGGCAGCAAGCTCGCGAAAGCCGAGGAACTCGGCATTCCGGTGCTGGACGAAGCAGGCATGCACACA
CTTCTGGAGGGCCACGCGCG

Downstream 100 bases:

>100_bases
CGCGGCGCGCCGGCGCGGGGCGGCGCCACGCGAACGCGTGCGCCGGGCGCGCGCGACGGTAGGACGCCCGCGATCGCAAT
GGGGCGTTGGGGTGTTTTCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIREILKMGDPRLLEVARPVEAFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVIIFGFGSSERYPEAPPVPETVL
VNPSIEYLPPDLEEGWEGCLSVPGLRGVVSRYRRVRYSGFDQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSK
FGFADVLFPGLDAQADD

Sequences:

>Translated_177_residues
MIREILKMGDPRLLEVARPVEAFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVIIFGFGSSERYPEAPPVPETVL
VNPSIEYLPPDLEEGWEGCLSVPGLRGVVSRYRRVRYSGFDQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSK
FGFADVLFPGLDAQADD
>Mature_177_residues
MIREILKMGDPRLLEVARPVEAFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVIIFGFGSSERYPEAPPVPETVL
VNPSIEYLPPDLEEGWEGCLSVPGLRGVVSRYRRVRYSGFDQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSK
FGFADVLFPGLDAQADD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=34.8571428571429, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=40.9395973154362, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645728, Length=182, Percent_Identity=33.5164835164835, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645726, Length=181, Percent_Identity=34.2541436464088, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19697; Mature: 19697

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILKMGDPRLLEVARPVEAFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVII
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGSSERYPEAPPVPETVLVNPSIEYLPPDLEEGWEGCLSVPGLRGVVSRYRRVRYSGF
EECCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
DQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSKFGFADVLFPGLDAQADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIREILKMGDPRLLEVARPVEAFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVII
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGSSERYPEAPPVPETVLVNPSIEYLPPDLEEGWEGCLSVPGLRGVVSRYRRVRYSGF
EECCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
DQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSKFGFADVLFPGLDAQADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]