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The map label for this gene is sbp [H]

Identifier: 53719452

GI number: 53719452

Start: 2193033

End: 2194016

Strand: Reverse

Name: sbp [H]

Synonym: BPSL1839

Alternate gene names: 53719452

Gene position: 2194016-2193033 (Counterclockwise)

Preceding gene: 53719456

Following gene: 53719451

Centisome position: 53.85

GC content: 66.87

Gene sequence:

>984_bases
TTGGCGCTCGGCGCGGCGGCGGCGCTCGGCGCGCTCACGCCGGCGCTCGCGGACACGACGTTCCTGAACGTTTCGTACGA
CCCGACGCGCGAGCTCTACCAGGACGTCAACCAGGCGTTCGGCAAGGAATGGAAGGCGAGGACGGGCGAGACGGTGAACT
TCAAGCAGTCGCACGGCGGCTCGGGCGCGCAGGCGCGCTCGGTGCTCGACGGGCTGCAGGCCGACGTGGTCACGCTCGCG
CTCGCGTACGACATCGACGCGCTCGCGAACAAGGGCCTCGTCAGCAAGGATTGGCAAAAGCGTCTGCCGGACAACGCGTC
GCCGTACACGTCGACGATCGTGTTCCTCGTGAGGAAGGGCAATCCGAAGGGCATCAAGGATTGGGACGATCTCGTGAAGC
CGGGCGTGTCGATCGTCACGCCGAACCCGAAAACCTCGGGCGGCGCGCGCTGGAACTACCTCGCCGCGTGGGCATACGCG
CAGCACCAGCCGGGCGGCACGGCGCAGACGGCGAAGGATTTCGTCACGAAGCTGTACAGGAACGCGGGCGTGCTCGACTC
GGGCGCGCGCGGCGCGACGACGAGCTTCGTGCAGCGCGGCATCGGCGACGTGCTGATCGCGTGGGAAAACGAGGCGTTCC
TGTCGATCAAGGAATTCGGCGCCGACAAGTTCGAGATCGTCGTGCCGTCGGCGAGCATTCTCGCGGAGCCGCCGGTGGCG
GTGGTCGACAAGGTGGTCGACAAGAAGGGCACGCGCAAGCTCGCCGACGCGTACCTGAACTTTCTGTACAGCAGGCAAGG
GCAGGAGATCGCCGCGCGCAACTACTACCGGCCGCGCTCGCGGGACGTGCCGGCGGCGCTCACGAAGCAGTTCCCGAAGC
TCAAGCTGTACACGGTCGACGACACGTTCGGCGGCTGGACCCAAGCGCAGAAGACGCATTTCGCCGACGGCGGCGTGTTC
GATTCGATCTACAAGCCGCAGTGA

Upstream 100 bases:

>100_bases
AACGAACGAACCGCAACGAACGACCCACGCATTTGGAGAACAACGGATGGTCAAGCGCAACACGGGGCTGGCAGGCGGCG
CGCGCCGTCTCATCGCATCA

Downstream 100 bases:

>100_bases
GCGAGGGCGAAGCGGCGCGCCGGACGGGCGCGCGGGCTTCGCGACAACACGAAATCGGCACGGCGGACGCGCGTTGCAGC
GCGTCCGCCGTGCCGTTGAA

Product: sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MALGAAAALGALTPALADTTFLNVSYDPTRELYQDVNQAFGKEWKARTGETVNFKQSHGGSGAQARSVLDGLQADVVTLA
LAYDIDALANKGLVSKDWQKRLPDNASPYTSTIVFLVRKGNPKGIKDWDDLVKPGVSIVTPNPKTSGGARWNYLAAWAYA
QHQPGGTAQTAKDFVTKLYRNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSIKEFGADKFEIVVPSASILAEPPVA
VVDKVVDKKGTRKLADAYLNFLYSRQGQEIAARNYYRPRSRDVPAALTKQFPKLKLYTVDDTFGGWTQAQKTHFADGGVF
DSIYKPQ

Sequences:

>Translated_327_residues
MALGAAAALGALTPALADTTFLNVSYDPTRELYQDVNQAFGKEWKARTGETVNFKQSHGGSGAQARSVLDGLQADVVTLA
LAYDIDALANKGLVSKDWQKRLPDNASPYTSTIVFLVRKGNPKGIKDWDDLVKPGVSIVTPNPKTSGGARWNYLAAWAYA
QHQPGGTAQTAKDFVTKLYRNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSIKEFGADKFEIVVPSASILAEPPVA
VVDKVVDKKGTRKLADAYLNFLYSRQGQEIAARNYYRPRSRDVPAALTKQFPKLKLYTVDDTFGGWTQAQKTHFADGGVF
DSIYKPQ
>Mature_326_residues
ALGAAAALGALTPALADTTFLNVSYDPTRELYQDVNQAFGKEWKARTGETVNFKQSHGGSGAQARSVLDGLQADVVTLAL
AYDIDALANKGLVSKDWQKRLPDNASPYTSTIVFLVRKGNPKGIKDWDDLVKPGVSIVTPNPKTSGGARWNYLAAWAYAQ
HQPGGTAQTAKDFVTKLYRNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSIKEFGADKFEIVVPSASILAEPPVAV
VDKVVDKKGTRKLADAYLNFLYSRQGQEIAARNYYRPRSRDVPAALTKQFPKLKLYTVDDTFGGWTQAQKTHFADGGVFD
SIYKPQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=323, Percent_Identity=65.015479876161, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1788765, Length=307, Percent_Identity=49.185667752443, Blast_Score=298, Evalue=5e-82,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35527; Mature: 35396

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGAAAALGALTPALADTTFLNVSYDPTRELYQDVNQAFGKEWKARTGETVNFKQSHGG
CCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCHHHHHCCCCEEEEHHCCCC
SGAQARSVLDGLQADVVTLALAYDIDALANKGLVSKDWQKRLPDNASPYTSTIVFLVRKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEEEEECC
NPKGIKDWDDLVKPGVSIVTPNPKTSGGARWNYLAAWAYAQHQPGGTAQTAKDFVTKLYR
CCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHEEEEHHHHCCCCCCHHHHHHHHHHHHH
NAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSIKEFGADKFEIVVPSASILAEPPVA
CCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEHHHCCCCEEEEEECCHHHHCCCCHH
VVDKVVDKKGTRKLADAYLNFLYSRQGQEIAARNYYRPRSRDVPAALTKQFPKLKLYTVD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEEC
DTFGGWTQAQKTHFADGGVFDSIYKPQ
CCCCCCHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALGAAAALGALTPALADTTFLNVSYDPTRELYQDVNQAFGKEWKARTGETVNFKQSHGG
CCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCHHHHHCCCCEEEEHHCCCC
SGAQARSVLDGLQADVVTLALAYDIDALANKGLVSKDWQKRLPDNASPYTSTIVFLVRKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEEEEECC
NPKGIKDWDDLVKPGVSIVTPNPKTSGGARWNYLAAWAYAQHQPGGTAQTAKDFVTKLYR
CCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHEEEEHHHHCCCCCCHHHHHHHHHHHHH
NAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSIKEFGADKFEIVVPSASILAEPPVA
CCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEHHHCCCCEEEEEECCHHHHCCCCHH
VVDKVVDKKGTRKLADAYLNFLYSRQGQEIAARNYYRPRSRDVPAALTKQFPKLKLYTVD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEEC
DTFGGWTQAQKTHFADGGVFDSIYKPQ
CCCCCCHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]