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The map label for this gene is estA [H]

Identifier: 53719379

GI number: 53719379

Start: 2080385

End: 2081071

Strand: Reverse

Name: estA [H]

Synonym: BPSL1765

Alternate gene names: 53719379

Gene position: 2081071-2080385 (Counterclockwise)

Preceding gene: 53719380

Following gene: 53719372

Centisome position: 51.07

GC content: 69.29

Gene sequence:

>687_bases
ATGCTCGACGAAACGCCGACGATCGAAATCGAGACCGGCCCGAACCCCGCCTTCGCGGTGATCCTGATGCACGGCCTCGG
CGCCGACGCGAACGATTTCGTGCCGCTCGTGCCCGAGTTGCGAATCGCGAACGGCCCGGCGGTGCGCTTCGTGTTCCCGA
ACGCGCCCGAGATCGCGGTCACCGCGAACAACGGCTACGTGATGCGCGCGTGGTACGACATCCTGTCGTTCGAAGGCGTG
AACCGCCAGGTCGACGAAGCCGGGATCGACGCATCGTGCGCGAGCGTGCGCGGCCTGATCGCCGAGCAGAACCGGCGCGG
CATCCCGACGTCGCGAATCTTCGTCGCGGGCTTCTCGCAGGGCGGCGCGATGGCGTACTCGGCGGGCCTCACGCACCCGG
ACGCGCTCGCGGGGCTGATCGTGCTGTCCGGCTACGTGCCTTCGCCGGGCTTCATCGACGCGCGGCTCGCCGACGCGAAT
CGCACGACGCCGATCTTCGCCGCGCACGGCACGGACGACGACATCCTGCCGATCCGCCTGGGCGAGGCCGCCCGCGATTT
CGCGCGCGACAAAGGCGCGAGCGTCGACTGGCACGCGTATCCGATGCCGCATTCGGTGTGCATCGAAGAAATCGACGCGC
TGCGCCGATGGCTGCATGCGCGGATCGCCGCGCTGCAAGCGGCGTGA

Upstream 100 bases:

>100_bases
ACACGGCCGCCCGTCGCGCGAGCAAGCGCGGCGCCGGTCATCCCGATGTGCGATCATCGACGCGTATTCTTCCGATCCCC
CATCCGAACGGAGCTCCAGC

Downstream 100 bases:

>100_bases
CCCGCCCGCCGGCGCGCCGGGGCTTTGTCCGCGCGCGGCGTCACGCCGCGTGAGACGCGCGCGGACGAGGCCGCGCCTCA
CGCGAGCGGCTTGCGCTTCG

Product: putative carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MLDETPTIEIETGPNPAFAVILMHGLGADANDFVPLVPELRIANGPAVRFVFPNAPEIAVTANNGYVMRAWYDILSFEGV
NRQVDEAGIDASCASVRGLIAEQNRRGIPTSRIFVAGFSQGGAMAYSAGLTHPDALAGLIVLSGYVPSPGFIDARLADAN
RTTPIFAAHGTDDDILPIRLGEAARDFARDKGASVDWHAYPMPHSVCIEEIDALRRWLHARIAALQAA

Sequences:

>Translated_228_residues
MLDETPTIEIETGPNPAFAVILMHGLGADANDFVPLVPELRIANGPAVRFVFPNAPEIAVTANNGYVMRAWYDILSFEGV
NRQVDEAGIDASCASVRGLIAEQNRRGIPTSRIFVAGFSQGGAMAYSAGLTHPDALAGLIVLSGYVPSPGFIDARLADAN
RTTPIFAAHGTDDDILPIRLGEAARDFARDKGASVDWHAYPMPHSVCIEEIDALRRWLHARIAALQAA
>Mature_228_residues
MLDETPTIEIETGPNPAFAVILMHGLGADANDFVPLVPELRIANGPAVRFVFPNAPEIAVTANNGYVMRAWYDILSFEGV
NRQVDEAGIDASCASVRGLIAEQNRRGIPTSRIFVAGFSQGGAMAYSAGLTHPDALAGLIVLSGYVPSPGFIDARLADAN
RTTPIFAAHGTDDDILPIRLGEAARDFARDKGASVDWHAYPMPHSVCIEEIDALRRWLHARIAALQAA

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI9966764, Length=203, Percent_Identity=33.0049261083744, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI5453722, Length=204, Percent_Identity=30.8823529411765, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI20270341, Length=209, Percent_Identity=24.4019138755981, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17508057, Length=207, Percent_Identity=36.7149758454106, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323147, Length=212, Percent_Identity=33.4905660377358, Blast_Score=125, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45553055, Length=206, Percent_Identity=31.0679611650485, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45553057, Length=206, Percent_Identity=31.0679611650485, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21357257, Length=206, Percent_Identity=31.0679611650485, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078179, Length=224, Percent_Identity=27.2321428571429, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24403; Mature: 24403

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDETPTIEIETGPNPAFAVILMHGLGADANDFVPLVPELRIANGPAVRFVFPNAPEIAV
CCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEE
TANNGYVMRAWYDILSFEGVNRQVDEAGIDASCASVRGLIAEQNRRGIPTSRIFVAGFSQ
ECCCCEEEEEEHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCC
GGAMAYSAGLTHPDALAGLIVLSGYVPSPGFIDARLADANRTTPIFAAHGTDDDILPIRL
CCEEEEECCCCCHHHHHHHHHEECCCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEEE
GEAARDFARDKGASVDWHAYPMPHSVCIEEIDALRRWLHARIAALQAA
CHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDETPTIEIETGPNPAFAVILMHGLGADANDFVPLVPELRIANGPAVRFVFPNAPEIAV
CCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEE
TANNGYVMRAWYDILSFEGVNRQVDEAGIDASCASVRGLIAEQNRRGIPTSRIFVAGFSQ
ECCCCEEEEEEHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCC
GGAMAYSAGLTHPDALAGLIVLSGYVPSPGFIDARLADANRTTPIFAAHGTDDDILPIRL
CCEEEEECCCCCHHHHHHHHHEECCCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEEE
GEAARDFARDKGASVDWHAYPMPHSVCIEEIDALRRWLHARIAALQAA
CHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA