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The map label for this gene is ltaA [H]

Identifier: 53719328

GI number: 53719328

Start: 2018070

End: 2019230

Strand: Reverse

Name: ltaA [H]

Synonym: BPSL1714

Alternate gene names: 53719328

Gene position: 2019230-2018070 (Counterclockwise)

Preceding gene: 53719329

Following gene: 53719327

Centisome position: 49.56

GC content: 71.58

Gene sequence:

>1161_bases
ATGACACGCACGACGGCGGCCGCGAAGGCGGCCGTCTCGACGACGAACCGGCGCAGCGAGGCCGGACGACCCAACCAATG
GATCGATGCGATGATTGATTTCCTGAGCGACACGGTGACGCTGCCGACCGCGGAGATGCGGCACGCGATGTTTACCGCGA
ATGTGGGCGACGATTGCTACGGCGAGGATCCGACGGTCAACGAGCTCGAATCGGTGGCGGCCGGACTGACCGGCAAGGAA
GCGGCGGCGTTCGTCACGAGCGGCACGCTCGGCAACCTGAGCGCGCTGCTCGCGCAATGCCCGCGCGGGCACGAGGTGAT
CCTCGGCGATCGCTCCGACCTGTACAACTACGAGGCGGGCGGCGTGTCGCTCGTCGGCGGCGCGGTGTTGCACCCCGTCG
AGACCGCCGACGACGGCAGCCTGCCGCTCGAGCGGCTGCGCGCGGCGATCCGCGACAAGCGCGACCCCCAGTGCGCGCCC
GCCGCGGTGATCGCGCTCGAGAATCCGCATTGCCTCGCCGGCGGCCGCGTGCTGTCGCTCGACTACCTGCGGCGCGTGCG
CGCGCTCGCCGACGAGCACGGGCTCGCCGTGCACATGGACGGCGCGCGTCTGTTCAACGCGCAGGCGAGCCTCGGCACGC
CGGCGGCCGAGATCGTCGCGCACGTCGATTCGGTCCAGTTCTGCCTGTCGAAGAGCCTCGCCGCGCCGTACGGCTCGATG
GTGTGCGGCAGCGCCGCCCTGATCGATCGCGTGAAGCGCTATCGGAAGCTGCTCGGCGGCGGCACGCGGCAAGCCGGCAT
CATGGCGGCCGCCGGGCTCGTCGCGCTGCGCACGATGGTCGCGCGGCTCGCGGACGATCACCGCCGCGCGGCGCGCCTCG
CCGCGGAGCTGGCGCGGATTCCGGGCGTCGCGCTGCGCTCGGCGGTGATCGAGACGAACATGGTGTTCTTCGACGTCGCC
GAGCCGGGCAACGAGGCGTTTCTCGCCGCGCTGCGCGACGCGGGCATCCGGATGGGCGTGCTCGGCGACGGCGTGATCCG
GGCCGTCGTGCACTACATGATCGACGACGACGCGATCAGCCGCACCGTCGACGCCGTCCGCGCGATTGTTCTTCCGTTCG
CCCCGGCGTTAGCGCCGGCCGCCGCATCGCAGGCGCAATGA

Upstream 100 bases:

>100_bases
GGCGCACGCGCGGCGGCGCGGGTGCGTCGCCGATCTGCTCGCGGCGGCGGCGGCCTGAGGCGGCCGCGCGGCCGCGCGCG
ATGCGCGGCGCGCGCATGCA

Downstream 100 bases:

>100_bases
TGAGCGCCTTCTCGCTGACCCACGCGCATCTCGATTTCGGCCATGAAGGAACCCGCATGACCACTTTTCCCATTTCCACC
GCGCGCCTCGTGCTGCGGCC

Product: putative threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTRTTAAAKAAVSTTNRRSEAGRPNQWIDAMIDFLSDTVTLPTAEMRHAMFTANVGDDCYGEDPTVNELESVAAGLTGKE
AAAFVTSGTLGNLSALLAQCPRGHEVILGDRSDLYNYEAGGVSLVGGAVLHPVETADDGSLPLERLRAAIRDKRDPQCAP
AAVIALENPHCLAGGRVLSLDYLRRVRALADEHGLAVHMDGARLFNAQASLGTPAAEIVAHVDSVQFCLSKSLAAPYGSM
VCGSAALIDRVKRYRKLLGGGTRQAGIMAAAGLVALRTMVARLADDHRRAARLAAELARIPGVALRSAVIETNMVFFDVA
EPGNEAFLAALRDAGIRMGVLGDGVIRAVVHYMIDDDAISRTVDAVRAIVLPFAPALAPAAASQAQ

Sequences:

>Translated_386_residues
MTRTTAAAKAAVSTTNRRSEAGRPNQWIDAMIDFLSDTVTLPTAEMRHAMFTANVGDDCYGEDPTVNELESVAAGLTGKE
AAAFVTSGTLGNLSALLAQCPRGHEVILGDRSDLYNYEAGGVSLVGGAVLHPVETADDGSLPLERLRAAIRDKRDPQCAP
AAVIALENPHCLAGGRVLSLDYLRRVRALADEHGLAVHMDGARLFNAQASLGTPAAEIVAHVDSVQFCLSKSLAAPYGSM
VCGSAALIDRVKRYRKLLGGGTRQAGIMAAAGLVALRTMVARLADDHRRAARLAAELARIPGVALRSAVIETNMVFFDVA
EPGNEAFLAALRDAGIRMGVLGDGVIRAVVHYMIDDDAISRTVDAVRAIVLPFAPALAPAAASQAQ
>Mature_385_residues
TRTTAAAKAAVSTTNRRSEAGRPNQWIDAMIDFLSDTVTLPTAEMRHAMFTANVGDDCYGEDPTVNELESVAAGLTGKEA
AAFVTSGTLGNLSALLAQCPRGHEVILGDRSDLYNYEAGGVSLVGGAVLHPVETADDGSLPLERLRAAIRDKRDPQCAPA
AVIALENPHCLAGGRVLSLDYLRRVRALADEHGLAVHMDGARLFNAQASLGTPAAEIVAHVDSVQFCLSKSLAAPYGSMV
CGSAALIDRVKRYRKLLGGGTRQAGIMAAAGLVALRTMVARLADDHRRAARLAAELARIPGVALRSAVIETNMVFFDVAE
PGNEAFLAALRDAGIRMGVLGDGVIRAVVHYMIDDDAISRTVDAVRAIVLPFAPALAPAAASQAQ

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=340, Percent_Identity=40.5882352941176, Blast_Score=244, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI193206522, Length=348, Percent_Identity=39.0804597701149, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI193206524, Length=348, Percent_Identity=39.0804597701149, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI193206520, Length=348, Percent_Identity=39.0804597701149, Blast_Score=222, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6320789, Length=353, Percent_Identity=33.9943342776204, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21355439, Length=370, Percent_Identity=38.3783783783784, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 40514; Mature: 40383

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTTAAAKAAVSTTNRRSEAGRPNQWIDAMIDFLSDTVTLPTAEMRHAMFTANVGDDCY
CCCCHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCC
GEDPTVNELESVAAGLTGKEAAAFVTSGTLGNLSALLAQCPRGHEVILGDRSDLYNYEAG
CCCCCHHHHHHHHHCCCCCHHHHEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GVSLVGGAVLHPVETADDGSLPLERLRAAIRDKRDPQCAPAAVIALENPHCLAGGRVLSL
CEEEECCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEECCCEEEEH
DYLRRVRALADEHGLAVHMDGARLFNAQASLGTPAAEIVAHVDSVQFCLSKSLAAPYGSM
HHHHHHHHHHHCCCEEEEECCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VCGSAALIDRVKRYRKLLGGGTRQAGIMAAAGLVALRTMVARLADDHRRAARLAAELARI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGVALRSAVIETNMVFFDVAEPGNEAFLAALRDAGIRMGVLGDGVIRAVVHYMIDDDAIS
CCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHH
RTVDAVRAIVLPFAPALAPAAASQAQ
HHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
TRTTAAAKAAVSTTNRRSEAGRPNQWIDAMIDFLSDTVTLPTAEMRHAMFTANVGDDCY
CCCHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCC
GEDPTVNELESVAAGLTGKEAAAFVTSGTLGNLSALLAQCPRGHEVILGDRSDLYNYEAG
CCCCCHHHHHHHHHCCCCCHHHHEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GVSLVGGAVLHPVETADDGSLPLERLRAAIRDKRDPQCAPAAVIALENPHCLAGGRVLSL
CEEEECCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEECCCEEEEH
DYLRRVRALADEHGLAVHMDGARLFNAQASLGTPAAEIVAHVDSVQFCLSKSLAAPYGSM
HHHHHHHHHHHCCCEEEEECCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VCGSAALIDRVKRYRKLLGGGTRQAGIMAAAGLVALRTMVARLADDHRRAARLAAELARI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGVALRSAVIETNMVFFDVAEPGNEAFLAALRDAGIRMGVLGDGVIRAVVHYMIDDDAIS
CCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHH
RTVDAVRAIVLPFAPALAPAAASQAQ
HHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.12 {DL-threo-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]