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The map label for this gene is pinE [H]

Identifier: 53719317

GI number: 53719317

Start: 1978747

End: 1979340

Strand: Reverse

Name: pinE [H]

Synonym: BPSL1702

Alternate gene names: 53719317

Gene position: 1979340-1978747 (Counterclockwise)

Preceding gene: 53719318

Following gene: 53719316

Centisome position: 48.58

GC content: 55.56

Gene sequence:

>594_bases
ATGAAAATAGGCTACGCTCGAGTCTCAACCGAAGAGCAGAATCTCGCACTGCAACGGGACGCCCTCATGCGTGCCGGATG
CAAGCGTATCTTTACTGATCGTGGCGTTTCCGGTACCGAGTTTGATCGCCCGGGGCTTAACCGCGCGCTCGCGAGCCTGC
GTGCAGGCGACACCTTAGTCGTCTGGCGGCTCGATCGCCTTGGCCGCGCACTCCCCAAGCTAGTCGGATTGGTTCAACAA
CTCTCGCTTAGGGAAATTCAATTCGAATCTCTTACCGAAGCCATGACAACCAATTCATCTAGCGGTATGCTCGTCTTCCA
CATGATGGCCGCACTCGCCCAGTTCGAGCGCTCTCTGATTAGCGAACGCACCTGCGCCGGCATGGCGGCAGCGCGGGCAC
GCGGCCAAATATTAGGGCGGCGGCCTGCATTAAACGAAAAGCAGAGAGCGCAGGCACTCAAACTTTTGCTGACACAACCA
ATCAAGTGTGTCGCGAAACAATTCAATGTTCATCCAAGAACTTTGCAGCGCCTCCAGAAAGCGCATCAAGCAACCGCGGT
TGCACTTCGGCCTAATAGCATCTTTCCGAAATAG

Upstream 100 bases:

>100_bases
GGCAAATCCAATACCCGATGGCCCCTCCCATCTCGACTCGACGGTTCACATACACTTCGAGATCGTATTTTTTCAAACCA
TTAGACGGCAAAACTCACTT

Downstream 100 bases:

>100_bases
AAAGAATATCGAGCAAAATCCTTATGGTTTCATCCTTTCTCGTGGCCGATCGTACATGGCCGTTCTTCTTTACGATATCC
AGACCGAAATTGATTTTAAT

Product: putative invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKIGYARVSTEEQNLALQRDALMRAGCKRIFTDRGVSGTEFDRPGLNRALASLRAGDTLVVWRLDRLGRALPKLVGLVQQ
LSLREIQFESLTEAMTTNSSSGMLVFHMMAALAQFERSLISERTCAGMAAARARGQILGRRPALNEKQRAQALKLLLTQP
IKCVAKQFNVHPRTLQRLQKAHQATAVALRPNSIFPK

Sequences:

>Translated_197_residues
MKIGYARVSTEEQNLALQRDALMRAGCKRIFTDRGVSGTEFDRPGLNRALASLRAGDTLVVWRLDRLGRALPKLVGLVQQ
LSLREIQFESLTEAMTTNSSSGMLVFHMMAALAQFERSLISERTCAGMAAARARGQILGRRPALNEKQRAQALKLLLTQP
IKCVAKQFNVHPRTLQRLQKAHQATAVALRPNSIFPK
>Mature_197_residues
MKIGYARVSTEEQNLALQRDALMRAGCKRIFTDRGVSGTEFDRPGLNRALASLRAGDTLVVWRLDRLGRALPKLVGLVQQ
LSLREIQFESLTEAMTTNSSSGMLVFHMMAALAQFERSLISERTCAGMAAARARGQILGRRPALNEKQRAQALKLLLTQP
IKCVAKQFNVHPRTLQRLQKAHQATAVALRPNSIFPK

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=52.247191011236, Blast_Score=168, Evalue=2e-43,
Organism=Escherichia coli, GI1787827, Length=180, Percent_Identity=33.8888888888889, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787638, Length=180, Percent_Identity=33.8888888888889, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21893; Mature: 21893

Theoretical pI: Translated: 11.82; Mature: 11.82

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGYARVSTEEQNLALQRDALMRAGCKRIFTDRGVSGTEFDRPGLNRALASLRAGDTLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEE
VWRLDRLGRALPKLVGLVQQLSLREIQFESLTEAMTTNSSSGMLVFHMMAALAQFERSLI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SERTCAGMAAARARGQILGRRPALNEKQRAQALKLLLTQPIKCVAKQFNVHPRTLQRLQK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AHQATAVALRPNSIFPK
HHHHHEEEECCCCCCCC
>Mature Secondary Structure
MKIGYARVSTEEQNLALQRDALMRAGCKRIFTDRGVSGTEFDRPGLNRALASLRAGDTLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEE
VWRLDRLGRALPKLVGLVQQLSLREIQFESLTEAMTTNSSSGMLVFHMMAALAQFERSLI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SERTCAGMAAARARGQILGRRPALNEKQRAQALKLLLTQPIKCVAKQFNVHPRTLQRLQK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AHQATAVALRPNSIFPK
HHHHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]