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The map label for this gene is dadA [H]

Identifier: 53719051

GI number: 53719051

Start: 1648302

End: 1649606

Strand: Reverse

Name: dadA [H]

Synonym: BPSL1415

Alternate gene names: 53719051

Gene position: 1649606-1648302 (Counterclockwise)

Preceding gene: 53719054

Following gene: 53719050

Centisome position: 40.49

GC content: 73.49

Gene sequence:

>1305_bases
ATGGATGTAATCGTCATCGGCGGCGGCATCGCCGGCATCGCCACCGCCTACCAGTTGCGCGCGGCCGGCCATCGCGTATG
CGTCGTCGAGCGCCACGCGACGGTCGCGCAAGGCGCGACCTACGGCCAGGGCGGCGTGCTGCTGCCGACGCCGCTCGACG
TCTGGTTCGGCCCGACGTTCATGCGCGAGCGGCGCGCGCAAAAGAGCGGCGTCGTCTACAAGCCGGGCCTGAACGGCGCG
CTGCGGCGCTTCACGCGCCGCCTCGACGCGCTGCGCGAGCCCGCCGCGTTCGCCGCGCAATACGCGCAGCTGCGCCCGCT
CGTCGAGCTGTCGCGCGAGGCGATCGCCGACGTCGAAGCGCGCTTCAATCTCGAATTCGAACAGAAGAGCGGCGTGCTGC
ACGTCGTGCGCGACGCGGCCGAATGGGACGCGACGCAGCCCGCGCTCGACCTGATGCGCGACCTCGGCCAGCCTTACGAG
ACGCTCACGCCCGACGCGTGCGCGGCGCTCGAACCGTCGATGCCCGTCGATCCCGCGTTCGCGGGCGGCGTGCTGCTCGC
GCAGGAGCGCACCGCGAACTGCCCGCTCTTCGCGAAGCTCGTCAAGCAGGTGCTCGACGAGCACGACGTGCAGTTCCGGC
TCGGCCGCGCGGTCGCGGCGATCCGCACCGACGCGCGACGCGCCGCCGTCGAGCTCGCGCCCGACGCGGCCGATCCGCGC
GCGGCGAAATCGAAGGAAGTGGACGTGATCTCCGCCGACGCGGTCGTCGTCGCGGCGGGCACCGAAAGCCTCGCGCTCGT
CGCGCCGCTCGGCGTGCGCGCGCCGCTGCATCCGGTGCGCATCCACACGCTGACGGCGCCCCTCGCGTATGAGGAGCACG
CGCCGCATCTGAGCATCGTCGATTCGGCCAAGCGGATCGCGATCACCCGGCTCGGCCAGCGGCTGCGAATCGGCGGCGGC
GCCGTGCTGCAAAGCGCGAAGGATCTCGCGCGGCCGCTGCCCGAGCCGCTGTCCGAAGCCGCGCTCGCGCTGCTCGGCCA
GGCGACGCACGACTGGCTGCCCGGCACCGCGAAGATCTCCGCCGCGCGCGCGTGGCAAGGCGCGAAGCTGCTGTCGCCGG
ACGGCCTGCCCGTCGTCGGGCCGACCGCCGATCCGCGGGTGTTCGTCAACTTCGGCCACGGCCCGGCGGGCTGGGGCCTC
GCGTTCGGGTCTGCTAAAGTGGTGGCAGATGACCTCGGCGGCGGCGCGCAGCGCGTGCCCGCCGACACGCTCGCCGCGCT
GCGCGCGGAGCGCTTTGCCACCTGA

Upstream 100 bases:

>100_bases
CTTCATTTGGCGCTATCATGCGCGCTTCACCGGGCCGCGCACGGCCTGCCACGCAAGATCCTCAATCGGGCCGGATGCGG
CGTCCGACATCGAATCGAAC

Downstream 100 bases:

>100_bases
CGCCGTCGCCGCCCGCGCGCGGGGCGGCGCGTGATCGCGAGCCGCCCATGATCCTGCCTGCCGCCTCCCCGCTGCCCCGT
TCGCTCGAACCGTTCGATGC

Product: putative D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDVIVIGGGIAGIATAYQLRAAGHRVCVVERHATVAQGATYGQGGVLLPTPLDVWFGPTFMRERRAQKSGVVYKPGLNGA
LRRFTRRLDALREPAAFAAQYAQLRPLVELSREAIADVEARFNLEFEQKSGVLHVVRDAAEWDATQPALDLMRDLGQPYE
TLTPDACAALEPSMPVDPAFAGGVLLAQERTANCPLFAKLVKQVLDEHDVQFRLGRAVAAIRTDARRAAVELAPDAADPR
AAKSKEVDVISADAVVVAAGTESLALVAPLGVRAPLHPVRIHTLTAPLAYEEHAPHLSIVDSAKRIAITRLGQRLRIGGG
AVLQSAKDLARPLPEPLSEAALALLGQATHDWLPGTAKISAARAWQGAKLLSPDGLPVVGPTADPRVFVNFGHGPAGWGL
AFGSAKVVADDLGGGAQRVPADTLAALRAERFAT

Sequences:

>Translated_434_residues
MDVIVIGGGIAGIATAYQLRAAGHRVCVVERHATVAQGATYGQGGVLLPTPLDVWFGPTFMRERRAQKSGVVYKPGLNGA
LRRFTRRLDALREPAAFAAQYAQLRPLVELSREAIADVEARFNLEFEQKSGVLHVVRDAAEWDATQPALDLMRDLGQPYE
TLTPDACAALEPSMPVDPAFAGGVLLAQERTANCPLFAKLVKQVLDEHDVQFRLGRAVAAIRTDARRAAVELAPDAADPR
AAKSKEVDVISADAVVVAAGTESLALVAPLGVRAPLHPVRIHTLTAPLAYEEHAPHLSIVDSAKRIAITRLGQRLRIGGG
AVLQSAKDLARPLPEPLSEAALALLGQATHDWLPGTAKISAARAWQGAKLLSPDGLPVVGPTADPRVFVNFGHGPAGWGL
AFGSAKVVADDLGGGAQRVPADTLAALRAERFAT
>Mature_434_residues
MDVIVIGGGIAGIATAYQLRAAGHRVCVVERHATVAQGATYGQGGVLLPTPLDVWFGPTFMRERRAQKSGVVYKPGLNGA
LRRFTRRLDALREPAAFAAQYAQLRPLVELSREAIADVEARFNLEFEQKSGVLHVVRDAAEWDATQPALDLMRDLGQPYE
TLTPDACAALEPSMPVDPAFAGGVLLAQERTANCPLFAKLVKQVLDEHDVQFRLGRAVAAIRTDARRAAVELAPDAADPR
AAKSKEVDVISADAVVVAAGTESLALVAPLGVRAPLHPVRIHTLTAPLAYEEHAPHLSIVDSAKRIAITRLGQRLRIGGG
AVLQSAKDLARPLPEPLSEAALALLGQATHDWLPGTAKISAARAWQGAKLLSPDGLPVVGPTADPRVFVNFGHGPAGWGL
AFGSAKVVADDLGGGAQRVPADTLAALRAERFAT

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=443, Percent_Identity=27.765237020316, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076
- InterPro:   IPR006036 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45896; Mature: 45896

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVIVIGGGIAGIATAYQLRAAGHRVCVVERHATVAQGATYGQGGVLLPTPLDVWFGPTF
CEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHCCCEECCCCEECCCCHHHHCCHHH
MRERRAQKSGVVYKPGLNGALRRFTRRLDALREPAAFAAQYAQLRPLVELSREAIADVEA
HHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFNLEFEQKSGVLHVVRDAAEWDATQPALDLMRDLGQPYETLTPDACAALEPSMPVDPAF
HEEEEEHHCCCEEEEHHHHHHCCCCCHHHHHHHHCCCCCHHCCCCHHHHCCCCCCCCCCC
AGGVLLAQERTANCPLFAKLVKQVLDEHDVQFRLGRAVAAIRTDARRAAVELAPDAADPR
CCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
AAKSKEVDVISADAVVVAAGTESLALVAPLGVRAPLHPVRIHTLTAPLAYEEHAPHLSIV
CCCCCCCEEEECCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCHHCCCCCCHHH
DSAKRIAITRLGQRLRIGGGAVLQSAKDLARPLPEPLSEAALALLGQATHDWLPGTAKIS
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEH
AARAWQGAKLLSPDGLPVVGPTADPRVFVNFGHGPAGWGLAFGSAKVVADDLGGGAQRVP
HHHCCCCCEEECCCCCEEECCCCCCEEEEECCCCCCCCCEECCCCEEEEECCCCCCCCCC
ADTLAALRAERFAT
HHHHHHHHHHHCCC
>Mature Secondary Structure
MDVIVIGGGIAGIATAYQLRAAGHRVCVVERHATVAQGATYGQGGVLLPTPLDVWFGPTF
CEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHCCCEECCCCEECCCCHHHHCCHHH
MRERRAQKSGVVYKPGLNGALRRFTRRLDALREPAAFAAQYAQLRPLVELSREAIADVEA
HHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFNLEFEQKSGVLHVVRDAAEWDATQPALDLMRDLGQPYETLTPDACAALEPSMPVDPAF
HEEEEEHHCCCEEEEHHHHHHCCCCCHHHHHHHHCCCCCHHCCCCHHHHCCCCCCCCCCC
AGGVLLAQERTANCPLFAKLVKQVLDEHDVQFRLGRAVAAIRTDARRAAVELAPDAADPR
CCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
AAKSKEVDVISADAVVVAAGTESLALVAPLGVRAPLHPVRIHTLTAPLAYEEHAPHLSIV
CCCCCCCEEEECCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCHHCCCCCCHHH
DSAKRIAITRLGQRLRIGGGAVLQSAKDLARPLPEPLSEAALALLGQATHDWLPGTAKIS
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEH
AARAWQGAKLLSPDGLPVVGPTADPRVFVNFGHGPAGWGLAFGSAKVVADDLGGGAQRVP
HHHCCCCCEEECCCCCEEECCCCCCEEEEECCCCCCCCCEECCCCEEEEECCCCCCCCCC
ADTLAALRAERFAT
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]