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The map label for this gene is tesA [H]

Identifier: 53719047

GI number: 53719047

Start: 1643189

End: 1643863

Strand: Reverse

Name: tesA [H]

Synonym: BPSL1411

Alternate gene names: 53719047

Gene position: 1643863-1643189 (Counterclockwise)

Preceding gene: 53719049

Following gene: 53719042

Centisome position: 40.34

GC content: 70.96

Gene sequence:

>675_bases
ATGAAACGCACAGTTCACTGGAAGGCCGTCGCGACGCTCGCCGTGGTCGCGACGTTCGCCGGCCCGGCATCCGCCGCCAC
GAATGCAGCGCCCGCGCCGAACGCCGACGGCGGCAAGCCGGTGATCGTCGTGCTCGGCGACAGCCTGTCGGCCGAATACG
GCCTGCCGCGCGATACGGGCTGGGTCGCGCTGATGCGCGCGCGCCTCGCGGCCGAGCGAATCGATTATAGCGTCGCGAAC
GCGAGCATCTCCGGCGACACGACGAGCGGCGGCCGCGCGCGGCTGCCCGCGGTGCTCGCACGGCTCAAGCCGGCCGTCGT
CGTCGTCGAACTCGGCGCGAACGACGCGCTGCGCGGCGTGCCGCTCGCCGCGACCGAAGGCAACCTGCGCGAGATCGTCG
CGCAGGCGCGGCAGGCGCGCGCGCAGGTGCTGCTCGTCGGCATGTACGTGCCGTCGAACTACGGCCCGGACTACACGCAA
AAGTTCCGCGGCGTCTACACGGGGCTGTCCAAGGAGATGCGCGTGCCGCTCGTGCCGTTCCTGCTCGCCGGCATCGAGAA
CAAGCCCGACATGTTCCAGGCCGACCAGATCCATCCGACGCAGGCCGCGCAGCGCCAGTTGCTCGACAACGTATGGCCGG
CCCTCAAGCCGCTGCTGCGGACGTCATCGCGCTGA

Upstream 100 bases:

>100_bases
CGTCAGGTCGATGCCAGCGAGAATCGTCAGCTCGCCGGCGGCATCGGCGACACGCTTGCACACATTCCGTACTTCGATGA
TTGGATCGGTAATCTTGAGC

Downstream 100 bases:

>100_bases
TCGCGCCGTGCCGGCATCTCGGTGCTTCGGTGCTTCGGTGCTTCGGTGCTTCGGTACTTCGGTACTTCGGTACTTCGGTA
CTTCGGTACTTCGGTACTTC

Product: putative acyl-CoA thioesterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKRTVHWKAVATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAERIDYSVAN
ASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYTQ
KFRGVYTGLSKEMRVPLVPFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSR

Sequences:

>Translated_224_residues
MKRTVHWKAVATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAERIDYSVAN
ASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYTQ
KFRGVYTGLSKEMRVPLVPFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSR
>Mature_224_residues
MKRTVHWKAVATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAERIDYSVAN
ASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYTQ
KFRGVYTGLSKEMRVPLVPFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSR

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=183, Percent_Identity=42.0765027322404, Blast_Score=140, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 23805; Mature: 23805

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTVHWKAVATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTG
CCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCH
WVALMRARLAAERIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
PLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYTQKFRGVYTGLSKEMRVPLVPF
CEECCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
LLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSR
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRTVHWKAVATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTG
CCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCH
WVALMRARLAAERIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
PLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYTQKFRGVYTGLSKEMRVPLVPF
CEECCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
LLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSR
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]