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The map label for this gene is purR [H]

Identifier: 53718940

GI number: 53718940

Start: 1519247

End: 1520281

Strand: Reverse

Name: purR [H]

Synonym: BPSL1304

Alternate gene names: 53718940

Gene position: 1520281-1519247 (Counterclockwise)

Preceding gene: 53718941

Following gene: 53718939

Centisome position: 37.31

GC content: 72.27

Gene sequence:

>1035_bases
ATGACGCCCACCATCAAAGACGTCGCCGCGCTCGCCGGCTTTTCGATCGCCACCGTGTCGCGCGCGATCAACGCGCCGCA
CACCGTCCATCCGGCGACGCTCGAAAAGATCCGCGCGGCGATCGGCGCGCTGCGCTTTCGCCCGAATCCGCTCGGCCGGC
AATTGCGCAGCGACCGCACGCAATTGATCGGCGTCGTGCTGCCGACGCTCGCGAATCCCGTGTTCGCCGAATGCCTGCAG
GGCGTCGACGAACTCGCGACGCAGGCCGGCTTCAAGCTGATCGTGATGTCGACCGAATACGATGCGGCGCGCGAGCGCCA
TGCGATCGAGACACTGCGCGCGCAGCGCGTGGAAGGGCTGATGCTCACCGTCGCCGACGCCGACGCGCACCCGCTGCTCG
ACGAGCTCGACCGCGACGGCCCGCTCTACGTGCTGATGCACAACGACACGCCGCATCGCCCGTCGGTGGCGGTCGACAAT
CGCCGCGCCGCGTACGACGGCGTGCGGATGCTGATCGAGCGCGGCCATCGGCGCGTGCTGATGCTCGCGGGCTCGCTCGA
CGCATCCGATCGCGCGCGGCTGCGCGTGCACGGCTATGCGCAGGCACTCGACGAGCGCGGGCTCGAACCGCTGCCCGCGC
TCGAGCTCGACTTCAATGCACCCGCGCTGCCGCACGCGATGCTCGCGCATCTGAGCGCGCGCGCGACGCGCCCCACCGCG
CTCTTCTGCAGCAACGACTGGCTCGCGATGGTCGTGATTCGCGGGCTGCGCGACGCGCACCTCGCGGTGCCCGACGACAT
GTCGGTGCTCGGCTTCGACGGCCTCGCGGTCGGCGAGCTGCTCGCGCCGCCGCTCGCGAGCGTCGCGACGCCGAATCGCG
AGATCGGCCGCGCCGCGTGGCGGCGCCTTGCCGAGCGCATCGCCGGCAAGCGCCATGCGCAACCCGCGCTGACGCTGCCG
CACGCGGTGCGCGACGGCGCGACCGTCGCGCCGCCGCGCGATGCGCGCGATGCGCGCGCGGCGCGCATCGCCTGA

Upstream 100 bases:

>100_bases
CAGCCGCGCCCGCTCGCCTTCGCCGCCGTCATCAGTTCTTCATCGACGCCGCCGATAATCCCGTCGACGAAAACGTTTAC
ACGCCTCCGCTTCCCGCTTC

Downstream 100 bases:

>100_bases
ACCGTGCTTTCGCTTTCCGTCGCTTTCCATTCGCTTCGCCCTCAAGGAGACCCGCCGTGACCGTCCGCCGTTCGCTTCTT
CCGCGCTCGCCGCTCGCCGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQ
GVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGLMLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDN
RRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGKRHAQPALTLP
HAVRDGATVAPPRDARDARAARIA

Sequences:

>Translated_344_residues
MTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQ
GVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGLMLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDN
RRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGKRHAQPALTLP
HAVRDGATVAPPRDARDARAARIA
>Mature_343_residues
TPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQG
VDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGLMLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDNR
RAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTAL
FCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGKRHAQPALTLPH
AVRDGATVAPPRDARDARAARIA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=30.8681672025724, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1790194, Length=321, Percent_Identity=33.6448598130841, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1790715, Length=344, Percent_Identity=27.3255813953488, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994940, Length=329, Percent_Identity=30.3951367781155, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1787948, Length=346, Percent_Identity=30.9248554913295, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788474, Length=296, Percent_Identity=32.0945945945946, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=31.864406779661, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=29.7058823529412, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=29.3604651162791, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=27.9503105590062, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=26.8072289156627, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786268, Length=329, Percent_Identity=24.3161094224924, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790689, Length=313, Percent_Identity=22.3642172523962, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37300; Mature: 37169

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRT
CCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QLIGVVLPTLANPVFAECLQGVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCE
MLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDNRRAAYDGVRMLIERGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEE
MLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
EEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEE
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAW
EEECCCHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHCCCHHHHCCCCHHHHHHHH
RRLAERIAGKRHAQPALTLPHAVRDGATVAPPRDARDARAARIA
HHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
TPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRT
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QLIGVVLPTLANPVFAECLQGVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCE
MLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDNRRAAYDGVRMLIERGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEE
MLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
EEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEE
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAW
EEECCCHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHCCCHHHHCCCCHHHHHHHH
RRLAERIAGKRHAQPALTLPHAVRDGATVAPPRDARDARAARIA
HHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA