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The map label for this gene is hisJ [H]

Identifier: 53718673

GI number: 53718673

Start: 1202831

End: 1203607

Strand: Reverse

Name: hisJ [H]

Synonym: BPSL1033

Alternate gene names: 53718673

Gene position: 1203607-1202831 (Counterclockwise)

Preceding gene: 53718675

Following gene: 53718672

Centisome position: 29.54

GC content: 65.89

Gene sequence:

>777_bases
ATGAAGAAGCTCGCCCTGTGCGCGGCCCTCGCCCTTGCGGCGGGCGGCGCCTTCGCGAAGGAATGGAAGACCGTGCGGAT
CGGCGTCGACGCCAGCTATCCGCCGTTCGAATCGACCGCGCCGAGCGGCGAGATCGTCGGATTCGACGTCGATCTCGCGA
AGGAAGTCTGCAAGCGGATCAACGCGAAGTGCACATGGACGCCGCAGGATCTCGACGGCATCATCCCCGCGCTGAAGGCG
AAGAAGTTCGACGTGATCGTGTCGTCGCTGACCGTCACCGACAAGCGCCGCGAGCAGATCGATTTCTCCGACAAGCTGTA
CGACGCGCCCGCGCGAATGATCGCGAAGGCGGGCTCGCCGCTCGCGCCGAGCGTCGAGTCGCTCAAGGGCAAGCGCGTCG
GCGTCGAGCAGGGCTCGACCCAGGAAACCTTCGCGAAGGCGCACTGGGAGCCGAAGGGCGTGACGATCGTGTCGTACCAG
AACCAGGATCAGGTGTATGCCGATCTCGGCTCGGGGCGTCTCGATGCGACGCTGCAAGACGAGTTGCAGGCCGACTACGG
CTTCCTGCGCACGCCGCGCGGCAAGGGCTTCGCCTGGGCAGGTCCGGCCGTGAAGGATCCGCGCACGCTCGGCGACGGCA
CCGCGATGGGCCTGCGCAAGGAAGACGCGGACCTGAAGGACGCGATCAACCGCGCGCTCGCTTCGATGCACAAGGACGGC
ACGTACGACAGGCTCTCGCGCAAGTACTTCCCGTACAGCGTGTATTCGGCGAAGTAA

Upstream 100 bases:

>100_bases
GATCGACGAACGCGAATTTTGGCCGCCTAGAATACGCCCGGCGCGCGCATGCCCGGACGCATCCGGCCGCGCGGCCGGGG
AAGTCAGGAAGAGGAACGAC

Downstream 100 bases:

>100_bases
GCGCCGAGCGGGCTGCGCCAAGCGACCGGCGCGCGGCCCGCGCAGCACCAGCGGTCGACACCAAGCGCCGCGATGCGCCG
CCGCCCTGCCCGAGCGCAGG

Product: histidine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLAKEVCKRINAKCTWTPQDLDGIIPALKA
KKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLAPSVESLKGKRVGVEQGSTQETFAKAHWEPKGVTIVSYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAWAGPAVKDPRTLGDGTAMGLRKEDADLKDAINRALASMHKDG
TYDRLSRKYFPYSVYSAK

Sequences:

>Translated_258_residues
MKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLAKEVCKRINAKCTWTPQDLDGIIPALKA
KKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLAPSVESLKGKRVGVEQGSTQETFAKAHWEPKGVTIVSYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAWAGPAVKDPRTLGDGTAMGLRKEDADLKDAINRALASMHKDG
TYDRLSRKYFPYSVYSAK
>Mature_258_residues
MKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLAKEVCKRINAKCTWTPQDLDGIIPALKA
KKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLAPSVESLKGKRVGVEQGSTQETFAKAHWEPKGVTIVSYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAWAGPAVKDPRTLGDGTAMGLRKEDADLKDAINRALASMHKDG
TYDRLSRKYFPYSVYSAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=55.4263565891473, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=56.6523605150215, Blast_Score=297, Evalue=4e-82,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=38.4920634920635, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=36.2549800796813, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1788228, Length=237, Percent_Identity=31.6455696202532, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=31.9672131147541, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28084; Mature: 28084

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLAKEVCKRI
CCHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHC
NAKCTWTPQDLDGIIPALKAKKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSP
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHCCCHHHHHHHHHHHHHHCCCC
LAPSVESLKGKRVGVEQGSTQETFAKAHWEPKGVTIVSYQNQDQVYADLGSGRLDATLQD
CCCCHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHH
ELQADYGFLRTPRGKGFAWAGPAVKDPRTLGDGTAMGLRKEDADLKDAINRALASMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TYDRLSRKYFPYSVYSAK
CHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLAKEVCKRI
CCHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHC
NAKCTWTPQDLDGIIPALKAKKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSP
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHCCCHHHHHHHHHHHHHHCCCC
LAPSVESLKGKRVGVEQGSTQETFAKAHWEPKGVTIVSYQNQDQVYADLGSGRLDATLQD
CCCCHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHH
ELQADYGFLRTPRGKGFAWAGPAVKDPRTLGDGTAMGLRKEDADLKDAINRALASMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TYDRLSRKYFPYSVYSAK
CHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]