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The map label for this gene is ydaP [H]

Identifier: 53718364

GI number: 53718364

Start: 832108

End: 833934

Strand: Reverse

Name: ydaP [H]

Synonym: BPSL0724

Alternate gene names: 53718364

Gene position: 833934-832108 (Counterclockwise)

Preceding gene: 53718369

Following gene: 53718363

Centisome position: 20.47

GC content: 69.46

Gene sequence:

>1827_bases
ATGGCAACTGTGGCCGATTTCATCGTCGAGCGGCTTTACGACTGGGGCGTGCGCCGCCTCTACGGCTACCCCGGCGACGG
CATCAACGGCATGTTCGGCGCGCTGAACCGCGCCGACGGCAAGATCGAATTCGTGCAGGTGCGACATGAGGAAATGGCGG
CATTCATGGCATGCGCGCATGCAAAATTTACAGGCGAGCTCGGCGTATGCATCGCGACCTCGGGCCCCGGCGCATCGCAT
CTGGTGACGGGGCTGTACGACGCGCGAATGGACCACATGCCGGTGCTTGCGATCGCAGGGCAGCAGGCGCGCGCGGCGCT
CGGCGGCCACTACCAGCAGGAGCTCGACCTGAGCGCGCTCTTCAAGGACGTCGCGGGCGCGTTCGTGCAGACGGCGAGCG
TGCCCGCGCAGGCGCGCCATCTCGTCGACCGCGCGGTGCGCACCGCGCTCGCCAACCGCACCGTCGTCGCGCTCGTGCTG
CCGAACGATCTGCAGGAGCTCGACTACGAGCCGCCGAAGCGCCGGCACGGCACCGTGCACTCCGGCATCGGCTATACCGA
TCCGCAGATCGTGCCGTACGAAGCCGACCTGCGCCGCGCGGCCGACGTGCTGAACGCGGGCTCGAAGGTCGCGATGCTCG
TCGGCGCGGGCGCGCTTCGCGCGACCGACGAAGTGATCGCGGTGGCCGACCGGCTCGGCGCGGGCGTCGCGAAGGCGCTG
CTCGGCAAGGCCGCGGTGCCCGACGAGCTGCCGTGGGTCACGGGCTCGATCGGGCTGCTCGGCACCGAGCCGAGTTACCG
GATGATGAGCGAATGCGACACGCTGCTCGTCGTCGGTTCGGGCTTTCCGTATTCCGAATTCCTGCCGAAGGAAGGCGAGG
CCCGCGGCGTGCAGATCGATCTGAAGGCGGACATGCTGAGCCTGCGCTATCCGATGGAGGTGAACCTCGTCGGCGACAGC
GCGCAGACGCTGCGCCGGCTGCTGCCGCTGCTCAACGAGCGCGCGGACGCGTCGTGGCGCGCGCAGATCGCGCGCTGGAA
CGCCGCATGGTGGAACACGCTCGACGCGCGCGCGCGCGTGAACCCCGGCAACGGGCGCGGCGTCAATCCGCAGCGCGCGT
TCACCGAGCTGTCGCCGCGCCTGCCCGAATACGCGATCGTGACGAGCGATTCGGGCTCCTGCGCGAACTGGTATGCGCGC
GACCTGCGGATGCGGCGCGGGATGATGTGCTCGCTGTCGGGCGGGCTCGCGTCGATGGGCGCGGCCGTGCCGTACGCGAT
CGCCGCGAAGTTCGCGCATCCGCACCGCCCGGTGATCGCGCTCGTCGGCGACGGCGCGATGCAGATGAACAACATGGCCG
AGCTGATCACGGTCGCGAAGTACTGGAAGCAATGGGCCGATCCGCGCTGGATCTGCATGGTGCTCAACAACGAGGATCTG
AACCAGGTCACGTGGGAGCAGCGCGTGATGGAGGGCGATCCGAAATTCGACGCGTCGCAGCAGGTGCCGGACGTGCCGTA
CCACCGCTTCGCCGAACTGATCGGCCTGAAGGGCGTGTACGTCGACGACGCCGACCAACTGGGCGCCGCGTGGGAGGACG
TGCTCGCAGCGAACCGGCCGGCCGTGCTCGAAGTGAAGTGCGACCCCGAGGTGCCGCCGCTGCCGCCGCACGTGACACTG
CAGCAGGCGAAGCATTTCGCGCAGGCGCTGATGCGCGGCGATCCGCGCGAGCGCAGCGTGATCGTCGAGACGGCGAAGCA
GGTGCTCTCCGGCGCGTTCTCCGGCCGCGCGGACCGGACGCGCGACGAGCCGGACGGCGACGCATGA

Upstream 100 bases:

>100_bases
CCACGGCGCGCATCGGGCATGGACGTTGCGCGACGCCCCATGCTCCCCGCCTCGGGTGCGAGACCCATCAAAACGGCGCG
AGCCGGAAAGGAGAAACAGC

Downstream 100 bases:

>100_bases
CGCGCGCGCGGCGCACGGACCGAACGAGCCTCACGCAATGAACGGCGCAAACATGTCGGTGAAACCCGCACAGCGGCCCG
AGCGCGGGTTCGCGAAGACG

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MATVADFIVERLYDWGVRRLYGYPGDGINGMFGALNRADGKIEFVQVRHEEMAAFMACAHAKFTGELGVCIATSGPGASH
LVTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLSALFKDVAGAFVQTASVPAQARHLVDRAVRTALANRTVVALVL
PNDLQELDYEPPKRRHGTVHSGIGYTDPQIVPYEADLRRAADVLNAGSKVAMLVGAGALRATDEVIAVADRLGAGVAKAL
LGKAAVPDELPWVTGSIGLLGTEPSYRMMSECDTLLVVGSGFPYSEFLPKEGEARGVQIDLKADMLSLRYPMEVNLVGDS
AQTLRRLLPLLNERADASWRAQIARWNAAWWNTLDARARVNPGNGRGVNPQRAFTELSPRLPEYAIVTSDSGSCANWYAR
DLRMRRGMMCSLSGGLASMGAAVPYAIAAKFAHPHRPVIALVGDGAMQMNNMAELITVAKYWKQWADPRWICMVLNNEDL
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGVYVDDADQLGAAWEDVLAANRPAVLEVKCDPEVPPLPPHVTL
QQAKHFAQALMRGDPRERSVIVETAKQVLSGAFSGRADRTRDEPDGDA

Sequences:

>Translated_608_residues
MATVADFIVERLYDWGVRRLYGYPGDGINGMFGALNRADGKIEFVQVRHEEMAAFMACAHAKFTGELGVCIATSGPGASH
LVTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLSALFKDVAGAFVQTASVPAQARHLVDRAVRTALANRTVVALVL
PNDLQELDYEPPKRRHGTVHSGIGYTDPQIVPYEADLRRAADVLNAGSKVAMLVGAGALRATDEVIAVADRLGAGVAKAL
LGKAAVPDELPWVTGSIGLLGTEPSYRMMSECDTLLVVGSGFPYSEFLPKEGEARGVQIDLKADMLSLRYPMEVNLVGDS
AQTLRRLLPLLNERADASWRAQIARWNAAWWNTLDARARVNPGNGRGVNPQRAFTELSPRLPEYAIVTSDSGSCANWYAR
DLRMRRGMMCSLSGGLASMGAAVPYAIAAKFAHPHRPVIALVGDGAMQMNNMAELITVAKYWKQWADPRWICMVLNNEDL
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGVYVDDADQLGAAWEDVLAANRPAVLEVKCDPEVPPLPPHVTL
QQAKHFAQALMRGDPRERSVIVETAKQVLSGAFSGRADRTRDEPDGDA
>Mature_607_residues
ATVADFIVERLYDWGVRRLYGYPGDGINGMFGALNRADGKIEFVQVRHEEMAAFMACAHAKFTGELGVCIATSGPGASHL
VTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLSALFKDVAGAFVQTASVPAQARHLVDRAVRTALANRTVVALVLP
NDLQELDYEPPKRRHGTVHSGIGYTDPQIVPYEADLRRAADVLNAGSKVAMLVGAGALRATDEVIAVADRLGAGVAKALL
GKAAVPDELPWVTGSIGLLGTEPSYRMMSECDTLLVVGSGFPYSEFLPKEGEARGVQIDLKADMLSLRYPMEVNLVGDSA
QTLRRLLPLLNERADASWRAQIARWNAAWWNTLDARARVNPGNGRGVNPQRAFTELSPRLPEYAIVTSDSGSCANWYARD
LRMRRGMMCSLSGGLASMGAAVPYAIAAKFAHPHRPVIALVGDGAMQMNNMAELITVAKYWKQWADPRWICMVLNNEDLN
QVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGVYVDDADQLGAAWEDVLAANRPAVLEVKCDPEVPPLPPHVTLQ
QAKHFAQALMRGDPRERSVIVETAKQVLSGAFSGRADRTRDEPDGDA

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=594, Percent_Identity=22.0538720538721, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1787096, Length=592, Percent_Identity=33.1081081081081, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87081685, Length=579, Percent_Identity=28.4974093264249, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=26.0176991150442, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=24.9557522123894, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788716, Length=586, Percent_Identity=22.5255972696246, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=27.1777003484321, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=577, Percent_Identity=21.4904679376083, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922626, Length=584, Percent_Identity=23.972602739726, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 66201; Mature: 66070

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVADFIVERLYDWGVRRLYGYPGDGINGMFGALNRADGKIEFVQVRHEEMAAFMACAH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHH
AKFTGELGVCIATSGPGASHLVTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLSAL
HCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHHHHHHHH
FKDVAGAFVQTASVPAQARHLVDRAVRTALANRTVVALVLPNDLQELDYEPPKRRHGTVH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCHHHCCCCC
SGIGYTDPQIVPYEADLRRAADVLNAGSKVAMLVGAGALRATDEVIAVADRLGAGVAKAL
CCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LGKAAVPDELPWVTGSIGLLGTEPSYRMMSECDTLLVVGSGFPYSEFLPKEGEARGVQID
HCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCEEEE
LKADMLSLRYPMEVNLVGDSAQTLRRLLPLLNERADASWRAQIARWNAAWWNTLDARARV
ECCCHHHEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCEEE
NPGNGRGVNPQRAFTELSPRLPEYAIVTSDSGSCANWYARDLRMRRGMMCSLSGGLASMG
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCHHHHH
AAVPYAIAAKFAHPHRPVIALVGDGAMQMNNMAELITVAKYWKQWADPRWICMVLNNEDL
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGVYVDDADQLGAAWEDVLAANRP
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCC
AVLEVKCDPEVPPLPPHVTLQQAKHFAQALMRGDPRERSVIVETAKQVLSGAFSGRADRT
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
RDEPDGDA
CCCCCCCC
>Mature Secondary Structure 
ATVADFIVERLYDWGVRRLYGYPGDGINGMFGALNRADGKIEFVQVRHEEMAAFMACAH
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHH
AKFTGELGVCIATSGPGASHLVTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLSAL
HCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHHHHHHHH
FKDVAGAFVQTASVPAQARHLVDRAVRTALANRTVVALVLPNDLQELDYEPPKRRHGTVH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCHHHCCCCC
SGIGYTDPQIVPYEADLRRAADVLNAGSKVAMLVGAGALRATDEVIAVADRLGAGVAKAL
CCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LGKAAVPDELPWVTGSIGLLGTEPSYRMMSECDTLLVVGSGFPYSEFLPKEGEARGVQID
HCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCEEEE
LKADMLSLRYPMEVNLVGDSAQTLRRLLPLLNERADASWRAQIARWNAAWWNTLDARARV
ECCCHHHEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCEEE
NPGNGRGVNPQRAFTELSPRLPEYAIVTSDSGSCANWYARDLRMRRGMMCSLSGGLASMG
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCHHHHH
AAVPYAIAAKFAHPHRPVIALVGDGAMQMNNMAELITVAKYWKQWADPRWICMVLNNEDL
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGVYVDDADQLGAAWEDVLAANRP
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCC
AVLEVKCDPEVPPLPPHVTLQQAKHFAQALMRGDPRERSVIVETAKQVLSGAFSGRADRT
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
RDEPDGDA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.0012 {thiamine} 0.03 {sodium} 2 {ferricyanide}} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]