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The map label for this gene is asnB [H]

Identifier: 53718325

GI number: 53718325

Start: 776411

End: 777943

Strand: Reverse

Name: asnB [H]

Synonym: BPSL0682

Alternate gene names: 53718325

Gene position: 777943-776411 (Counterclockwise)

Preceding gene: 53718326

Following gene: 53718324

Centisome position: 19.09

GC content: 64.06

Gene sequence:

>1533_bases
ATGTGCGGAATTGGGGCCATTTTCAGCACGCAGCGCGCGTCGGTCGACGGCATCGAGCGCTCGCTGAGCGCGTTGATGTC
GAAGATCGCGCACCGCGGCGACGCGTCGCGCTTCAACGAAAACCTGATCCGGGAGCGCTTCGGCCTCGCGACGAACCGCC
TCGCGATCGTCGAGCGCGACCATGCGCGCCAACCGGTGTCCGATCACGAGCGCGACCTGCACGTCGTGCTCAACGGGCAG
ATCTACAACTACAAGGAACTGCGCGACGAGCTCGCCGCGCGCGGCCACCGCTTCAGCCATGCGGGCGACGCCGAGGTGCT
GTTGCGCGCGTACCTCGAGTACGGCCCGTCGTTCGCGACGAAGCTCGACGGCATGTTCAGCTTCGTGCTGTTCGACAACC
TGAACGGAAAATTCCTGATCGGGCGCGACCACGTCGGCATCAAGCCGCTCTATTACGCGCAGCGCGACGGCGTCTGGTAC
GTCGCGTCGGAACTCAAGGCGCTCACGCACGTGAACGCGCGAATCGAGACGCTCGCGCCCGGCACGCTGTTCGACGGCAA
GGAGACGATCAGGTACGTGTCGTACGACGCCAGCCGCCCGGTGAGCCGCGACAGCTTCGACGAAGCGAAGGCGCGCGTGC
GCACGCTGCTCGAGGAATCGGTGCGCCGCCGCGTCGACACCGACCTGCCGATCTGCGTGATGTTCAGCGGCGGCATCGAC
AGCGCGATCGTGCTGCAGCTCGCGCACCGCTATCACCCGGACGTGACCGCGATCTCGTTCGGCCTGCCCGGCTCGAACGA
CATCGAGATCGCGCACCGCTACTGCGCCGAGCACGGCATCCGCCACGTGATCTACACGTTCACGAAGCAGGACCTGCTCG
AGAACATCGACGACGCGGTCTATTACGGCGAATTCTTCGAGAAGATCGACGCGATCGATTCGGCGATCGCGCATTTCGGC
TACTACATCGCGAACAAGCTCGGCTTGAAGGTTGCGCTCTGCGGCGAAGGCAGCGACGAGATCTTCGGCGGCTACGATCT
GTTCAAGACGCACGCGCAGCCCGGCGCGCTGTCGCTCTATCGGCTCAACAACCTGCACCGCACCGATCTGCAGCGCGTCG
ATCGCGCGAGCATGCGCAATACGGTCGAGGCGCGGGTGCCGTTCATGGACGCCGAGCTGATCGACTACGTGCTGAGCCTC
GACTTCTCGTACAAACTGCACGGCGGCGTCGAAAAGCATATCCTGCGCGAGGCGTTTCGCGACGTGCTGCCGGGCTACAT
CATCGATCGCCCGAAAATCAGGATGCCCGACGGCAGCGGCGTGAAGAACGTCATCATCGACCACATCGACAGCCTCGGCG
ACGCGCCGCCTGACGATGCGGCAAGGCGGCTCGGCGAAATCGGGCTGTCCGATCGCTCGGCGCTCTTCTTCGCGCGGAAG
TACCTGGAGTGCAACTTCCCGTTTCCGACGCAGCGCTTCAAGCAGGCCGGCAAGGATTTTTCGGAATCCGGATATTTCGA
CTTCATCTCATGA

Upstream 100 bases:

>100_bases
ATCGGCGCGAACGTCGACGGGCTGATCTACCCGAACGTCGCCGCGGCGCGCGCATGAACGCGGCCCGCGCCATCGAATCC
ACTCACGAGAGAGAAATGAC

Downstream 100 bases:

>100_bases
CGCACGGGCGCGGCGCCTCGCCGCGCCCGTCCGGGGAATGCCCATGAAGACTTTTCTGTTCGTCGAAGCCCGCCCGATCG
AATGCGCGGGCATCGGCTAC

Product: putative asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MCGIGAIFSTQRASVDGIERSLSALMSKIAHRGDASRFNENLIRERFGLATNRLAIVERDHARQPVSDHERDLHVVLNGQ
IYNYKELRDELAARGHRFSHAGDAEVLLRAYLEYGPSFATKLDGMFSFVLFDNLNGKFLIGRDHVGIKPLYYAQRDGVWY
VASELKALTHVNARIETLAPGTLFDGKETIRYVSYDASRPVSRDSFDEAKARVRTLLEESVRRRVDTDLPICVMFSGGID
SAIVLQLAHRYHPDVTAISFGLPGSNDIEIAHRYCAEHGIRHVIYTFTKQDLLENIDDAVYYGEFFEKIDAIDSAIAHFG
YYIANKLGLKVALCGEGSDEIFGGYDLFKTHAQPGALSLYRLNNLHRTDLQRVDRASMRNTVEARVPFMDAELIDYVLSL
DFSYKLHGGVEKHILREAFRDVLPGYIIDRPKIRMPDGSGVKNVIIDHIDSLGDAPPDDAARRLGEIGLSDRSALFFARK
YLECNFPFPTQRFKQAGKDFSESGYFDFIS

Sequences:

>Translated_510_residues
MCGIGAIFSTQRASVDGIERSLSALMSKIAHRGDASRFNENLIRERFGLATNRLAIVERDHARQPVSDHERDLHVVLNGQ
IYNYKELRDELAARGHRFSHAGDAEVLLRAYLEYGPSFATKLDGMFSFVLFDNLNGKFLIGRDHVGIKPLYYAQRDGVWY
VASELKALTHVNARIETLAPGTLFDGKETIRYVSYDASRPVSRDSFDEAKARVRTLLEESVRRRVDTDLPICVMFSGGID
SAIVLQLAHRYHPDVTAISFGLPGSNDIEIAHRYCAEHGIRHVIYTFTKQDLLENIDDAVYYGEFFEKIDAIDSAIAHFG
YYIANKLGLKVALCGEGSDEIFGGYDLFKTHAQPGALSLYRLNNLHRTDLQRVDRASMRNTVEARVPFMDAELIDYVLSL
DFSYKLHGGVEKHILREAFRDVLPGYIIDRPKIRMPDGSGVKNVIIDHIDSLGDAPPDDAARRLGEIGLSDRSALFFARK
YLECNFPFPTQRFKQAGKDFSESGYFDFIS
>Mature_510_residues
MCGIGAIFSTQRASVDGIERSLSALMSKIAHRGDASRFNENLIRERFGLATNRLAIVERDHARQPVSDHERDLHVVLNGQ
IYNYKELRDELAARGHRFSHAGDAEVLLRAYLEYGPSFATKLDGMFSFVLFDNLNGKFLIGRDHVGIKPLYYAQRDGVWY
VASELKALTHVNARIETLAPGTLFDGKETIRYVSYDASRPVSRDSFDEAKARVRTLLEESVRRRVDTDLPICVMFSGGID
SAIVLQLAHRYHPDVTAISFGLPGSNDIEIAHRYCAEHGIRHVIYTFTKQDLLENIDDAVYYGEFFEKIDAIDSAIAHFG
YYIANKLGLKVALCGEGSDEIFGGYDLFKTHAQPGALSLYRLNNLHRTDLQRVDRASMRNTVEARVPFMDAELIDYVLSL
DFSYKLHGGVEKHILREAFRDVLPGYIIDRPKIRMPDGSGVKNVIIDHIDSLGDAPPDDAARRLGEIGLSDRSALFFARK
YLECNFPFPTQRFKQAGKDFSESGYFDFIS

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=511, Percent_Identity=30.7240704500978, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI168229252, Length=511, Percent_Identity=30.7240704500978, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI168229250, Length=511, Percent_Identity=30.7240704500978, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI296010848, Length=483, Percent_Identity=30.2277432712215, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI296010852, Length=406, Percent_Identity=30.2955665024631, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI296010850, Length=406, Percent_Identity=30.2955665024631, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1786889, Length=528, Percent_Identity=32.9545454545455, Blast_Score=230, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17560178, Length=479, Percent_Identity=30.6889352818372, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71993933, Length=482, Percent_Identity=30.7053941908714, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25147557, Length=482, Percent_Identity=30.7053941908714, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25147560, Length=214, Percent_Identity=32.7102803738318, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321563, Length=501, Percent_Identity=30.1397205588822, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6325403, Length=496, Percent_Identity=30.241935483871, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI45553209, Length=536, Percent_Identity=29.4776119402985, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 57538; Mature: 57538

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIGAIFSTQRASVDGIERSLSALMSKIAHRGDASRFNENLIRERFGLATNRLAIVERD
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECC
HARQPVSDHERDLHVVLNGQIYNYKELRDELAARGHRFSHAGDAEVLLRAYLEYGPSFAT
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHH
KLDGMFSFVLFDNLNGKFLIGRDHVGIKPLYYAQRDGVWYVASELKALTHVNARIETLAP
HHHHHEEEEEEECCCCEEEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCHHEECCC
GTLFDGKETIRYVSYDASRPVSRDSFDEAKARVRTLLEESVRRRVDTDLPICVMFSGGID
CCCCCCHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
SAIVLQLAHRYHPDVTAISFGLPGSNDIEIAHRYCAEHGIRHVIYTFTKQDLLENIDDAV
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YYGEFFEKIDAIDSAIAHFGYYIANKLGLKVALCGEGSDEIFGGYDLFKTHAQPGALSLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEE
RLNNLHRTDLQRVDRASMRNTVEARVPFMDAELIDYVLSLDFSYKLHGGVEKHILREAFR
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
DVLPGYIIDRPKIRMPDGSGVKNVIIDHIDSLGDAPPDDAARRLGEIGLSDRSALFFARK
HHCCCCEECCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
YLECNFPFPTQRFKQAGKDFSESGYFDFIS
HHCCCCCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIGAIFSTQRASVDGIERSLSALMSKIAHRGDASRFNENLIRERFGLATNRLAIVERD
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECC
HARQPVSDHERDLHVVLNGQIYNYKELRDELAARGHRFSHAGDAEVLLRAYLEYGPSFAT
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHH
KLDGMFSFVLFDNLNGKFLIGRDHVGIKPLYYAQRDGVWYVASELKALTHVNARIETLAP
HHHHHEEEEEEECCCCEEEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCHHEECCC
GTLFDGKETIRYVSYDASRPVSRDSFDEAKARVRTLLEESVRRRVDTDLPICVMFSGGID
CCCCCCHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
SAIVLQLAHRYHPDVTAISFGLPGSNDIEIAHRYCAEHGIRHVIYTFTKQDLLENIDDAV
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YYGEFFEKIDAIDSAIAHFGYYIANKLGLKVALCGEGSDEIFGGYDLFKTHAQPGALSLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEE
RLNNLHRTDLQRVDRASMRNTVEARVPFMDAELIDYVLSLDFSYKLHGGVEKHILREAFR
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
DVLPGYIIDRPKIRMPDGSGVKNVIIDHIDSLGDAPPDDAARRLGEIGLSDRSALFFARK
HHCCCCEECCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
YLECNFPFPTQRFKQAGKDFSESGYFDFIS
HHCCCCCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]