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The map label for this gene is nasD [H]

Identifier: 53718152

GI number: 53718152

Start: 564531

End: 566975

Strand: Reverse

Name: nasD [H]

Synonym: BPSL0512

Alternate gene names: 53718152

Gene position: 566975-564531 (Counterclockwise)

Preceding gene: 53718154

Following gene: 53718151

Centisome position: 13.92

GC content: 67.08

Gene sequence:

>2445_bases
ATGCGGAAAATGAAGCTGGTGATGGTCGGCAACGGCATGGCCGGAGTGCGCACGCTCGAAGAATTGTTCAAGCTCGCGCC
CGATTTGTACGACGTGACGGTATTCGGCGCCGAACCGCATCCGAACTACAACCGGATCCTGCTGTCGCCCGTGCTCGCCG
GCGAGCAGACGCTCGAGCAGATCGTCCTCAACGATTACGCATGGTACGCATCGCACGGCGTCACGCTCCACGTCGGCAAG
AAGATCGTGAAGATCGATCGCATGAAGCGCGTCGTGATCGCCGACGACGGCACCGAGGCCGCATACGACCGGCTGCTGCT
CGCGACCGGCTCGAACCCGTTCATGCTGCCCGTTCCGGGCGCGCAGCTCGACGGCGTGCTCGGCTATCGCGACATCGCGG
ACACGCAGGCGATGATCGACGCCGCCGCGCGGCACACGCACGCGGTCGTGATCGGCGGCGGCCTGCTCGGCCTCGAGGCG
GCGAACGGCCTGAAGCTGCGCGGCATGGACGTGACGGTCGTGCACCTCGCGCCGACGCTGCTCGAGCGCCAGCTCGACGC
GAAGGCGGGCGGCCTGTTGCGTGCGTCGCTGGAGGCGCGCGGGCTGACGTTTCTGATGCCGAAGCAAACGCAGGCGCTCG
TCGGCGACGAGCACGGCCGCGTGCGCGCGGTGCACTTCAAGGACGGCGGCGAATGCAAGGCCGACCTCGTCGTGATGGCG
GTCGGCATTCGCCCGAACACGGCGCTCGCCGAAAGCGCGGGCCTCTATTGCAATCGCGGGATCGTCGTCAACGACACGAT
GCAGACCTACGATCCGCGCATCTACGCGGTCGGCGAATGCGTGAGCCACCGCGGTATCGCGTACGGCCTCGTCGCGCCGC
TGTTCGAACAGGCGAAAGTCGCGGCGAACCATCTCGCGCAGTTCGGCATCGGCCGCTACACGGGCTCGGTGACGTCGACG
AAGCTCAAGGTCACGGGCATCGACCTGTTCTCCGCGGGCGACTTCCTCGGCGGCGACGATACCGAAGACATCACGCTGTC
CGATCCGATCGCGGGCGTCTACAAGAAGCTCGTGATCAAGGACGACAGGATCGTCGGCGCGTGCCTGTACGGCGACACGG
CCGACGGCGCGTGGTATTTCAAGCTGCTGCGCGAAGGCCGCAACATCGCGGACATCCGCGAGACGCTGATGTTCGGCGAG
ACGAGCCTCGGCGACACCGGCCACAGCGGCGCGACGCGCGCGATGACGATGGCCGACGACGCCGAGGTGTGCGGCTGCAA
CGGCGTGTGCAAGGGCACGATCGTATCGGCGATCAAGGAAAAAGGCCTCTTCACGCTCGACGACGTGCGCAAGCACACGA
AGGCGTCGGCGTCATGCGGCTCGTGCACCGGCCTCGTCGAGCAGATCCTGATGTCGACGCTCGGCGGCGACTATTCGGCG
TCGCCGAAGGCGAAGCCCGTATGCGGCTGCACCGATCATACGCACGCCGAAGTGCGCGCGGCGATCCGCGAGCACAAGCT
GCTGTCGGTGCCGGAGGCGATGCGCTTTCTCGAATGGCGCACGCCGAACGGCTGCTCGAGCTGCCGCCCGGCGCTCAACT
ACTACTGCATCAGCACGTGGCCGCACGACGCGAAGGACGATCCGCAGTCGCGCTTCATCAACGAGCGCGCGCACGCGAAC
ATCCAGAAGGACGGCACGTATTCGGTCGTGCCGCGGATGTGGGGCGGCGTGACGAGCGCCGACGAGTTGCGCCGCATCGC
CGACGTCGTCGACAAGTACGCGATCCCGACCGTCAAGGTGACGGGCGGCCAGCGCATCGATCTGCTCGGCGTGAAGAAGG
AGGACCTGCCCGGCGTGTGGCGCGACCTCGGCATGCCGAGCGGCCACGCGTACGCGAAGGCGCTGCGCACGGTGAAGACC
TGCGTCGGCTCCGAATGGTGCCGCTTCGGCACGCAGGATTCGACGCTGATGGGCCAGCAGCTCGAACGCGCGCTGTGGCG
GATGTACGCGCCGCACAAGGTGAAGCTCGCGGTGTCCGGCTGCCCGCGCAACTGCGCGGAATCGGGGATCAAGGACGTCG
GCGTGATCGGCGTCGATTCGGGCTGGGAGATCTACGTCGGCGGCAACGGCGGCATCAAGACCGAAGTCGCGCAGTTCTTC
TGCAAGGTGAAGACGCACGAGGAGGTGCTCGAATATGCGGGCGCGTTCCTGCAGCTGTATCGCGAGGAAGGCTGGTATCT
CGAGCGCACCGTGCACTATCTCGAGCGCGTCGGCCTCGACCACGTGAAGGCGCGCGTGCTCGACGACGCCGAGAACCGCC
GCGCGCTGTGGGACAGGCTGCAGTTCGCGCTGAAGGACGAGCCGGACCCGTGGCACGACACGCAGGACGCGAAGGTCGAT
CTGCGCCAGTTCATCCCGATCGCCGTCGCGCCCGCCGATGCGTGA

Upstream 100 bases:

>100_bases
TTTTAAATAGCGGCCAAATAAAAAGTGGCATGGCAATTGCTGTTTTCCGTCCATCGAACATGGGCCCCCACGACAAGCGG
CCCCAGTCAGGAGATCCCCG

Downstream 100 bases:

>100_bases
TGCCGATGCGCGATGCGTGACGCGTCGCGCATCGCGCCGCACGCGCACCCCGTCAATCACCCAGGAATCCGAATCATGTC
CGCCACCCAACCCGAATGGA

Product: putative nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MRKMKLVMVGNGMAGVRTLEELFKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQIVLNDYAWYASHGVTLHVGK
KIVKIDRMKRVVIADDGTEAAYDRLLLATGSNPFMLPVPGAQLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEA
ANGLKLRGMDVTVVHLAPTLLERQLDAKAGGLLRASLEARGLTFLMPKQTQALVGDEHGRVRAVHFKDGGECKADLVVMA
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYTGSVTST
KLKVTGIDLFSAGDFLGGDDTEDITLSDPIAGVYKKLVIKDDRIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGE
TSLGDTGHSGATRAMTMADDAEVCGCNGVCKGTIVSAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTLGGDYSA
SPKAKPVCGCTDHTHAEVRAAIREHKLLSVPEAMRFLEWRTPNGCSSCRPALNYYCISTWPHDAKDDPQSRFINERAHAN
IQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKVTGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKT
CVGSEWCRFGTQDSTLMGQQLERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAQFF
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWDRLQFALKDEPDPWHDTQDAKVD
LRQFIPIAVAPADA

Sequences:

>Translated_814_residues
MRKMKLVMVGNGMAGVRTLEELFKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQIVLNDYAWYASHGVTLHVGK
KIVKIDRMKRVVIADDGTEAAYDRLLLATGSNPFMLPVPGAQLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEA
ANGLKLRGMDVTVVHLAPTLLERQLDAKAGGLLRASLEARGLTFLMPKQTQALVGDEHGRVRAVHFKDGGECKADLVVMA
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYTGSVTST
KLKVTGIDLFSAGDFLGGDDTEDITLSDPIAGVYKKLVIKDDRIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGE
TSLGDTGHSGATRAMTMADDAEVCGCNGVCKGTIVSAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTLGGDYSA
SPKAKPVCGCTDHTHAEVRAAIREHKLLSVPEAMRFLEWRTPNGCSSCRPALNYYCISTWPHDAKDDPQSRFINERAHAN
IQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKVTGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKT
CVGSEWCRFGTQDSTLMGQQLERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAQFF
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWDRLQFALKDEPDPWHDTQDAKVD
LRQFIPIAVAPADA
>Mature_814_residues
MRKMKLVMVGNGMAGVRTLEELFKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQIVLNDYAWYASHGVTLHVGK
KIVKIDRMKRVVIADDGTEAAYDRLLLATGSNPFMLPVPGAQLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEA
ANGLKLRGMDVTVVHLAPTLLERQLDAKAGGLLRASLEARGLTFLMPKQTQALVGDEHGRVRAVHFKDGGECKADLVVMA
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYTGSVTST
KLKVTGIDLFSAGDFLGGDDTEDITLSDPIAGVYKKLVIKDDRIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGE
TSLGDTGHSGATRAMTMADDAEVCGCNGVCKGTIVSAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTLGGDYSA
SPKAKPVCGCTDHTHAEVRAAIREHKLLSVPEAMRFLEWRTPNGCSSCRPALNYYCISTWPHDAKDDPQSRFINERAHAN
IQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKVTGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKT
CVGSEWCRFGTQDSTLMGQQLERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAQFF
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWDRLQFALKDEPDPWHDTQDAKVD
LRQFIPIAVAPADA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=280, Percent_Identity=30, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI21389617, Length=280, Percent_Identity=30, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI226437568, Length=280, Percent_Identity=30, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI4757732, Length=228, Percent_Identity=28.5087719298246, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI22202629, Length=228, Percent_Identity=28.5087719298246, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1789765, Length=831, Percent_Identity=35.2587244283995, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1789065, Length=302, Percent_Identity=28.8079470198675, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1788892, Length=288, Percent_Identity=27.0833333333333, Blast_Score=93, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=407, Percent_Identity=25.0614250614251, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI281359715, Length=244, Percent_Identity=27.8688524590164, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI281359713, Length=244, Percent_Identity=27.8688524590164, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639250, Length=244, Percent_Identity=27.8688524590164, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18543267, Length=244, Percent_Identity=27.8688524590164, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639252, Length=244, Percent_Identity=27.8688524590164, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639257, Length=244, Percent_Identity=27.8688524590164, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585130, Length=319, Percent_Identity=22.884012539185, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24581020, Length=211, Percent_Identity=29.3838862559242, Blast_Score=78, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28573993, Length=211, Percent_Identity=29.3838862559242, Blast_Score=78, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358499, Length=199, Percent_Identity=31.6582914572864, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88740; Mature: 88740

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKMKLVMVGNGMAGVRTLEELFKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQ
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEHHHCCHHHHHH
IVLNDYAWYASHGVTLHVGKKIVKIDRMKRVVIADDGTEAAYDRLLLATGSNPFMLPVPG
HHHHHHHEECCCCEEEECCCHHHHHHHCCEEEEECCCCHHHHCEEEEEECCCCEEEECCC
AQLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEAANGLKLRGMDVTVVHLAPTL
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCEEEECCCEEEEEHHHHH
LERQLDAKAGGLLRASLEARGLTFLMPKQTQALVGDEHGRVRAVHFKDGGECKADLVVMA
HHHHHCCCCCCEEEEEHHHCCEEEECCCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEEE
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKV
ECCCCCCHHHHCCCCEECCCEEECCCHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHH
AANHLAQFGIGRYTGSVTSTKLKVTGIDLFSAGDFLGGDDTEDITLSDPIAGVYKKLVIK
HHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHEE
DDRIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGETSLGDTGHSGATRAMTMADD
CCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
AEVCGCNGVCKGTIVSAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTLGGDYSA
CCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
SPKAKPVCGCTDHTHAEVRAAIREHKLLSVPEAMRFLEWRTPNGCSSCRPALNYYCISTW
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCCCCEEEEECC
PHDAKDDPQSRFINERAHANIQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKV
CCCCCCCHHHHHHHHHHCCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
TGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTLMGQQ
CCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHH
LERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAQFF
HHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHH
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWDRL
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHH
QFALKDEPDPWHDTQDAKVDLRQFIPIAVAPADA
HHHHCCCCCCCCCCCCCHHHHHHCCCEEEECCCC
>Mature Secondary Structure
MRKMKLVMVGNGMAGVRTLEELFKLAPDLYDVTVFGAEPHPNYNRILLSPVLAGEQTLEQ
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEHHHCCHHHHHH
IVLNDYAWYASHGVTLHVGKKIVKIDRMKRVVIADDGTEAAYDRLLLATGSNPFMLPVPG
HHHHHHHEECCCCEEEECCCHHHHHHHCCEEEEECCCCHHHHCEEEEEECCCCEEEECCC
AQLDGVLGYRDIADTQAMIDAAARHTHAVVIGGGLLGLEAANGLKLRGMDVTVVHLAPTL
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCEEEECCCEEEEEHHHHH
LERQLDAKAGGLLRASLEARGLTFLMPKQTQALVGDEHGRVRAVHFKDGGECKADLVVMA
HHHHHCCCCCCEEEEEHHHCCEEEECCCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEEE
VGIRPNTALAESAGLYCNRGIVVNDTMQTYDPRIYAVGECVSHRGIAYGLVAPLFEQAKV
ECCCCCCHHHHCCCCEECCCEEECCCHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHH
AANHLAQFGIGRYTGSVTSTKLKVTGIDLFSAGDFLGGDDTEDITLSDPIAGVYKKLVIK
HHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHEE
DDRIVGACLYGDTADGAWYFKLLREGRNIADIRETLMFGETSLGDTGHSGATRAMTMADD
CCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
AEVCGCNGVCKGTIVSAIKEKGLFTLDDVRKHTKASASCGSCTGLVEQILMSTLGGDYSA
CCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
SPKAKPVCGCTDHTHAEVRAAIREHKLLSVPEAMRFLEWRTPNGCSSCRPALNYYCISTW
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCCCCEEEEECC
PHDAKDDPQSRFINERAHANIQKDGTYSVVPRMWGGVTSADELRRIADVVDKYAIPTVKV
CCCCCCCHHHHHHHHHHCCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
TGGQRIDLLGVKKEDLPGVWRDLGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTLMGQQ
CCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHH
LERALWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAQFF
HHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHH
CKVKTHEEVLEYAGAFLQLYREEGWYLERTVHYLERVGLDHVKARVLDDAENRRALWDRL
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHH
QFALKDEPDPWHDTQDAKVDLRQFIPIAVAPADA
HHHHCCCCCCCCCCCCCHHHHHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]