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The map label for this gene is gcvA [H]

Identifier: 53718051

GI number: 53718051

Start: 445227

End: 446192

Strand: Reverse

Name: gcvA [H]

Synonym: BPSL0411

Alternate gene names: 53718051

Gene position: 446192-445227 (Counterclockwise)

Preceding gene: 53718055

Following gene: 53718040

Centisome position: 10.95

GC content: 71.33

Gene sequence:

>966_bases
ATGAACATCCATCGACTGCCGATGCTGAATGCGCTGCGCGTGTTCGAGGCCGCCGCGCGGCACGAAAGCTTCTCGCGCGC
GGCAGACGAGTTGTCCGTCACGCACGGCGCGGTCAGCCACCAGATGCGCGCGCTGGAAGCCGAGCTCGGCGTGCCGCTGT
TCGTGCGCCACGGCAAGCGGCTCGCGCTGACGGACGCGGGCGGCCGCTACGCGCAGCAGGTGCGCGCCGCGCTCGCGCTG
CTCGCCGACGCGACCCGCGAGGTTCGCGCGAGCGAGCGCGACAAGCGGCTCGTCGTGTCGACGCTGCCGTCGTTCGCCGC
GCGCTGGATCACGCCGAGGATCGGCCCGTTCATCGAACGGCATCCGGAAATCGACCTGGAGCTGCGGGCAAGCGATTCGC
TCGTCGATTTCGCGCGCGACGACGTCGATGTCGCGATCCGCTTCGGCCACGGCGTCTATCCGGGGCTGCACGTCGAGCCG
CTGCTCGACGAGACGTTCTTTCCCGTCTGCGCGCCGACGCTCAACGGCGGCATGCTGCCCGAGACGCCCGCCGATCTCGT
CCGCTATCCGCTGCTGCGCTCGGACGACGAGCTGTGGCGGCCGTGGTTCGACGCGGCGGGGCTCGACACGCTGACCGAGC
CGAAGCGCGGCGTGCTGTATCAGGATTCGTCGAATCTGCTGCAGGCGGCGATCGACGGCCAGGGCATCGCGCTCGTGCGG
CGCTCGCTCGCGGTGCCGGAGGTGGCGGCCGGCCGGATCGTGCGGCTCTTCGACATCGCGGGGCCGAGCCCGTGGCACTA
CTTCTTCGTGTGCCCGCCGTCGCTCGCGCAAACGCCGCGCGTGCAGGCGCTCAGGAGCTGGCTGCTGGACGAGATCGCGC
GCTTCAGGGCGCTGTGCGCGGCGCAGGAGGCGCAGCACACGGCGGCCTATGCGGCCGCGCGTGCGCGCGGCAAGGAGGAA
AACTAG

Upstream 100 bases:

>100_bases
GACAGCCTCCGCGAAATCGCTTCAGTGGCTACAATCGTAGTTCCCGCAAGGCTTTGCGCCAAACGATCGATTCTCACGTG
CTTGTGAGAAAAACTAACCG

Downstream 100 bases:

>100_bases
CGGCCGGCCGTCACAGGACGACCTTGACCATCGGATGGCCCGTCAGCGCGCGGTAGATGTTGTCGAGCTGCGCCTGGCTC
GTCGCGCGCACGGTGATCGT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNIHRLPMLNALRVFEAAARHESFSRAADELSVTHGAVSHQMRALEAELGVPLFVRHGKRLALTDAGGRYAQQVRAALAL
LADATREVRASERDKRLVVSTLPSFAARWITPRIGPFIERHPEIDLELRASDSLVDFARDDVDVAIRFGHGVYPGLHVEP
LLDETFFPVCAPTLNGGMLPETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
RSLAVPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQALRSWLLDEIARFRALCAAQEAQHTAAYAAARARGKEE
N

Sequences:

>Translated_321_residues
MNIHRLPMLNALRVFEAAARHESFSRAADELSVTHGAVSHQMRALEAELGVPLFVRHGKRLALTDAGGRYAQQVRAALAL
LADATREVRASERDKRLVVSTLPSFAARWITPRIGPFIERHPEIDLELRASDSLVDFARDDVDVAIRFGHGVYPGLHVEP
LLDETFFPVCAPTLNGGMLPETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
RSLAVPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQALRSWLLDEIARFRALCAAQEAQHTAAYAAARARGKEE
N
>Mature_321_residues
MNIHRLPMLNALRVFEAAARHESFSRAADELSVTHGAVSHQMRALEAELGVPLFVRHGKRLALTDAGGRYAQQVRAALAL
LADATREVRASERDKRLVVSTLPSFAARWITPRIGPFIERHPEIDLELRASDSLVDFARDDVDVAIRFGHGVYPGLHVEP
LLDETFFPVCAPTLNGGMLPETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
RSLAVPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQALRSWLLDEIARFRALCAAQEAQHTAAYAAARARGKEE
N

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=42.9553264604811, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=34.1379310344828, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.6122448979592, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=30.3135888501742, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=28.7625418060201, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=37.5757575757576, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081978, Length=264, Percent_Identity=26.8939393939394, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=32.6241134751773, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789440, Length=263, Percent_Identity=23.574144486692, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788748, Length=167, Percent_Identity=30.5389221556886, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788887, Length=162, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=207, Percent_Identity=26.0869565217391, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=252, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35549; Mature: 35549

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHRLPMLNALRVFEAAARHESFSRAADELSVTHGAVSHQMRALEAELGVPLFVRHGKR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
LALTDAGGRYAQQVRAALALLADATREVRASERDKRLVVSTLPSFAARWITPRIGPFIER
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHC
HPEIDLELRASDSLVDFARDDVDVAIRFGHGVYPGLHVEPLLDETFFPVCAPTLNGGMLP
CCCCCEEEECCCCHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHCCCCHHCCCCCCCCCC
ETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
CCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHH
RSLAVPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQALRSWLLDEIARFRALCA
HHHCCCCHHHCCEEEEEECCCCCCCEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
AQEAQHTAAYAAARARGKEEN
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNIHRLPMLNALRVFEAAARHESFSRAADELSVTHGAVSHQMRALEAELGVPLFVRHGKR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
LALTDAGGRYAQQVRAALALLADATREVRASERDKRLVVSTLPSFAARWITPRIGPFIER
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHC
HPEIDLELRASDSLVDFARDDVDVAIRFGHGVYPGLHVEPLLDETFFPVCAPTLNGGMLP
CCCCCEEEECCCCHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHCCCCHHCCCCCCCCCC
ETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
CCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHH
RSLAVPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQALRSWLLDEIARFRALCA
HHHCCCCHHHCCEEEEEECCCCCCCEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
AQEAQHTAAYAAARARGKEEN
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]