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The map label for this gene is gcvA [H]

Identifier: 53717924

GI number: 53717924

Start: 296548

End: 297429

Strand: Reverse

Name: gcvA [H]

Synonym: BPSL0283

Alternate gene names: 53717924

Gene position: 297429-296548 (Counterclockwise)

Preceding gene: 53717927

Following gene: 161723214

Centisome position: 7.3

GC content: 70.52

Gene sequence:

>882_bases
ATGGCACGCGATCTTCCACCGTTTCCGGCCTTAAGGGCCTTCGAAGCAGCAGCGAGGCACGAAAGCTTCAGCGCCGCGGG
CGAGGAGCTGCATGTGACTCACGGTGCCATCAGCCGGCAGATCGCCGCATTCGAGGCGTGGCTCGGCAAGCCGGTGTTCC
ACCGGCACGGCAAGCGGGTGAAGCTCACCGACGAAGGCCGCCGCTATCTCGCCACCGTCGAGGCGGCGTTCGACAGCATC
GCGGGCGCGACCGTCCAGCTGCGCGAGACGGGCGCGGCGCGCGTGCTGCGGATCAACGCGCTGCCGACGTTCGCGATGAA
GTGGCTGCTGCCGCGGCTGTCGCGGTTTCAGCGCGAGGCGCCGAACGTCGAGCTGAAGCTGTCGACGTCGAATGCGCCGC
TCGGCTCGCTCGACGGGTTCGATGTCGCGATCCGGCGCGGCCCCGGCCGCTGGCCGAACTGCACGAGCGGGCGCTTTCTC
GACGAGAGCGTGATTCCCGTGTGCAGCGCGGCGCTCCTGAAGCGCGCACCGATCGCGAGCGCGGACGATCTCGCGCGCCA
TGTGCTGCTGCATTCGGATACGCGCCCGGATGCGTGGCGCGAGTGGTTCGCCGCGGCGGGCGCGCCGCTCAAGGGGCGCA
AGAAGCAATCGTTCGACCACTTCTATCTGGCGTTGCAGGCGGCCGTGGACGGCCTCGGCGTCGCGCTCGGGCCGCTGCCG
CTCATCGCCGACGAACTCGCGAGCGGCCGGCTCGTGACGCCGCTGCCGGGCCCGCGTATCGCAACGCGCAGCTACTGGTG
GATCGCGCCGCGCGAGACGGCGAACGACGTGCTCGTCGGCCGGTTCTGTGCATGGCTCGACGCGCAGGCCCAGGCGAGCT
GA

Upstream 100 bases:

>100_bases
CATGGCAGTCCTCGTGATTTCCACGAGCCGAGATTACGCCCGTTCGCCGCGGACTCCAAACGATTTTATCGGCGCGCCCT
TGTGAGCTAGAATCACAAGC

Downstream 100 bases:

>100_bases
GCGCTCGCGCGGATTTCAGCGAGTCATCGGGGATCGCGGACGAGCGAGCGGGCGGCGCGCGCCAATGTCGATGGCGATGG
CGATGACGATGGCACAACCA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRVKLTDEGRRYLATVEAAFDSI
AGATVQLRETGAARVLRINALPTFAMKWLLPRLSRFQREAPNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPNCTSGRFL
DESVIPVCSAALLKRAPIASADDLARHVLLHSDTRPDAWREWFAAAGAPLKGRKKQSFDHFYLALQAAVDGLGVALGPLP
LIADELASGRLVTPLPGPRIATRSYWWIAPRETANDVLVGRFCAWLDAQAQAS

Sequences:

>Translated_293_residues
MARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRVKLTDEGRRYLATVEAAFDSI
AGATVQLRETGAARVLRINALPTFAMKWLLPRLSRFQREAPNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPNCTSGRFL
DESVIPVCSAALLKRAPIASADDLARHVLLHSDTRPDAWREWFAAAGAPLKGRKKQSFDHFYLALQAAVDGLGVALGPLP
LIADELASGRLVTPLPGPRIATRSYWWIAPRETANDVLVGRFCAWLDAQAQAS
>Mature_292_residues
ARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRVKLTDEGRRYLATVEAAFDSIA
GATVQLRETGAARVLRINALPTFAMKWLLPRLSRFQREAPNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPNCTSGRFLD
ESVIPVCSAALLKRAPIASADDLARHVLLHSDTRPDAWREWFAAAGAPLKGRKKQSFDHFYLALQAAVDGLGVALGPLPL
IADELASGRLVTPLPGPRIATRSYWWIAPRETANDVLVGRFCAWLDAQAQAS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=35.5932203389831, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=33.2155477031802, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=29.2096219931272, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=29.9342105263158, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786401, Length=248, Percent_Identity=27.4193548387097, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787128, Length=139, Percent_Identity=33.8129496402878, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI157672245, Length=191, Percent_Identity=29.8429319371728, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=25.7692307692308, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32009; Mature: 31878

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLATVEAAFDSIAGATVQLRETGAARVLRINALPTFAMKWLLPRLSRFQREA
EECCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHC
PNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPNCTSGRFLDESVIPVCSAALLKRAPIAS
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
ADDLARHVLLHSDTRPDAWREWFAAAGAPLKGRKKQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHCCHH
LIADELASGRLVTPLPGPRIATRSYWWIAPRETANDVLVGRFCAWLDAQAQAS
HHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARDLPPFPALRAFEAAARHESFSAAGEELHVTHGAISRQIAAFEAWLGKPVFHRHGKRV
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLATVEAAFDSIAGATVQLRETGAARVLRINALPTFAMKWLLPRLSRFQREA
EECCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHC
PNVELKLSTSNAPLGSLDGFDVAIRRGPGRWPNCTSGRFLDESVIPVCSAALLKRAPIAS
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
ADDLARHVLLHSDTRPDAWREWFAAAGAPLKGRKKQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHCCHH
LIADELASGRLVTPLPGPRIATRSYWWIAPRETANDVLVGRFCAWLDAQAQAS
HHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]