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The map label for this gene is rlmI [H]

Identifier: 53717849

GI number: 53717849

Start: 215294

End: 216571

Strand: Reverse

Name: rlmI [H]

Synonym: BPSL0207

Alternate gene names: 53717849

Gene position: 216571-215294 (Counterclockwise)

Preceding gene: 53717850

Following gene: 53717848

Centisome position: 5.32

GC content: 70.42

Gene sequence:

>1278_bases
ATGCATACCGTTACGCTCAAGCCGTCGAAAGACAAATCCCTGCTGCGCCGCCACCCGTGGGTCTACGCGAACGCGATCGA
CTGCGTCGACGGCAAGCCCGCGCCCGGCGCGACCGTCATCGTGCGCGCGCACGACGGGCGCTTTCTCGCGCGCGCCGCGT
ACAGCCCGCATTCGCAGATCCGGCTGCGCGTGTGGAGCTTCGACGAGAACGAGCCGATCGACCACGCGTTCTTCAAGCGG
CGCGTGCAACGCGCGCTCGCGCATCGCCGCGCGATGATCTCGGGCACGGACGCGGTGCGGCTCGTGTTCGGCGAGGCGGA
CGGGTTGCCGGGGCTCATCGTCGATTACTACGTCGCGGCGCGAGGCGCCGCCCACACCGGCGACGCCGCCGCGCGCGCGG
CCGAAGGCGGCGCGGCCGCGCCGGTCGCGCCGGGCGACGGCGAGGGTCGCGGCCAGCTCGTCTGCCAGTTCATGGCGGCG
GGCGTCGAGCACTGGAAGGGCGCGATCGTCGCGGCGATCGTCGCGGCCACCGGCTGCCCGAACGTCTATGAGCGCTCGGA
CGTGTCGATCCGCGAAAAGGAAGGGCTCGAACAGACGACCGGCGTGCTCGCGGGCGACGCGCCGCCCGACACGCTGATCG
CGAACGAGAACGGCGTGCTGTATCACGTCGACGTGCGCAACGGCCACAAGACGGGCTTCTACGTCGACCAGCGCGAGAAC
CGCGCACTCGTCGCGCAGTACGCGCGCGATCGCGACGTGCTGAACTGCTTCTGCTACACGGGCGGCTTCTCGCTCGCGGC
GCTCAAGGGCGGCGCGAAGCGGGTCGTGTCGATCGATTCGTCGGGCGACGCGCTCGCGCTCGCGCAGCGCAACGTCGCCG
CGAACGGCTTCGACGCCGCGCGCGCGCAATGGCTCGACGCCGACGCGTTCAAGACGCTGCGCCGCCTCGTCGACGAAGGC
GAGCGCTTCGACCTGATCGTGCTCGATCCGCCGAAGTTCGCGCCGACGCGCGACAGCGTCGATCGCGCGGCGCGCGCGTA
CAAGGACGTCAATCTGAGCGGCTTGAAGCTGCTGCGCCCGGGCGGCCTGCTGTTCACGTACTCGTGCTCCGGCGCGATCG
ACATGGACCTGTTCCAGAAGATCGTCGCGGGCGCGGCGGCCGACGCGAAGGTCGACGCGCGCATCCTCAAGCGGCTCGGC
GCGGGCGTCGATCATCCGCTGCTGACCGCGTTCCCCGAAGGGGAATATCTGAAGGGGCTGCTGTTGCAAATCGCGTGA

Upstream 100 bases:

>100_bases
AAGCGCGATTGACCCGCGCCGCCGCGCGCGCCGCATCACTGAATCGACGAATCCCAGAATCAGACTCCGCCGCGCCCCGC
GCGGCCATTTCGATCCCGAC

Downstream 100 bases:

>100_bases
TCGCCCCGATCTGAGCGGGCGGCGCTGTGCACGCCCGCGCCGCGCCGGCCGTTCGGCCGACAGCGGGGGCTTGACAGATA
TGTTTCAATAAATGGCTGAG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MHTVTLKPSKDKSLLRRHPWVYANAIDCVDGKPAPGATVIVRAHDGRFLARAAYSPHSQIRLRVWSFDENEPIDHAFFKR
RVQRALAHRRAMISGTDAVRLVFGEADGLPGLIVDYYVAARGAAHTGDAAARAAEGGAAAPVAPGDGEGRGQLVCQFMAA
GVEHWKGAIVAAIVAATGCPNVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQREN
RALVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARAQWLDADAFKTLRRLVDEG
ERFDLIVLDPPKFAPTRDSVDRAARAYKDVNLSGLKLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLG
AGVDHPLLTAFPEGEYLKGLLLQIA

Sequences:

>Translated_425_residues
MHTVTLKPSKDKSLLRRHPWVYANAIDCVDGKPAPGATVIVRAHDGRFLARAAYSPHSQIRLRVWSFDENEPIDHAFFKR
RVQRALAHRRAMISGTDAVRLVFGEADGLPGLIVDYYVAARGAAHTGDAAARAAEGGAAAPVAPGDGEGRGQLVCQFMAA
GVEHWKGAIVAAIVAATGCPNVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQREN
RALVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARAQWLDADAFKTLRRLVDEG
ERFDLIVLDPPKFAPTRDSVDRAARAYKDVNLSGLKLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLG
AGVDHPLLTAFPEGEYLKGLLLQIA
>Mature_425_residues
MHTVTLKPSKDKSLLRRHPWVYANAIDCVDGKPAPGATVIVRAHDGRFLARAAYSPHSQIRLRVWSFDENEPIDHAFFKR
RVQRALAHRRAMISGTDAVRLVFGEADGLPGLIVDYYVAARGAAHTGDAAARAAEGGAAAPVAPGDGEGRGQLVCQFMAA
GVEHWKGAIVAAIVAATGCPNVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQREN
RALVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARAQWLDADAFKTLRRLVDEG
ERFDLIVLDPPKFAPTRDSVDRAARAYKDVNLSGLKLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLG
AGVDHPLLTAFPEGEYLKGLLLQIA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=419, Percent_Identity=42.9594272076372, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787181, Length=213, Percent_Identity=29.1079812206573, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45654; Mature: 45654

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTVTLKPSKDKSLLRRHPWVYANAIDCVDGKPAPGATVIVRAHDGRFLARAAYSPHSQI
CCEEECCCCCCHHHHHHCCCEEECEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCEE
RLRVWSFDENEPIDHAFFKRRVQRALAHRRAMISGTDAVRLVFGEADGLPGLIVDYYVAA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHH
RGAAHTGDAAARAAEGGAAAPVAPGDGEGRGQLVCQFMAAGVEHWKGAIVAAIVAATGCP
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQREN
CCCCCCCCCCHHHCCCHHHHCEEECCCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCC
RALVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAA
CHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCHH
RAQWLDADAFKTLRRLVDEGERFDLIVLDPPKFAPTRDSVDRAARAYKDVNLSGLKLLRP
HHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC
GGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAGVDHPLLTAFPEGEYLKGL
CCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
LLQIA
HHCCC
>Mature Secondary Structure
MHTVTLKPSKDKSLLRRHPWVYANAIDCVDGKPAPGATVIVRAHDGRFLARAAYSPHSQI
CCEEECCCCCCHHHHHHCCCEEECEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCEE
RLRVWSFDENEPIDHAFFKRRVQRALAHRRAMISGTDAVRLVFGEADGLPGLIVDYYVAA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHH
RGAAHTGDAAARAAEGGAAAPVAPGDGEGRGQLVCQFMAAGVEHWKGAIVAAIVAATGCP
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQREN
CCCCCCCCCCHHHCCCHHHHCEEECCCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCC
RALVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAA
CHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCHH
RAQWLDADAFKTLRRLVDEGERFDLIVLDPPKFAPTRDSVDRAARAYKDVNLSGLKLLRP
HHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC
GGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAGVDHPLLTAFPEGEYLKGL
CCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
LLQIA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA