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The map label for this gene is nagP [H]

Identifier: 52788024

GI number: 52788024

Start: 4197457

End: 4198830

Strand: Direct

Name: nagP [H]

Synonym: BLi04352

Alternate gene names: 52788024

Gene position: 4197457-4198830 (Clockwise)

Preceding gene: 52788023

Following gene: 52788029

Centisome position: 99.4

GC content: 51.09

Gene sequence:

>1374_bases
ATGTTGGGATTTCTTCAAAATATCGGCCGCGCGCTCATGCTGCCGATTGCCGTTCTTCCTGCTGCGGGCCTCCTGCTCGC
ACTGGGACGGGAAGACCTGTTAAACATTCCGTTTATCGCTGCTTCAGGACAATCAATTCTTGACCAACTTCCGATTATCT
TTGCGATAGGCGTTGCGATCGGATTGTCAAAGGATGGCCACGGGGCCGCCGCTCTGTCCGGAGCCATCGGTTATTTTGTC
CTGACAGGCGGCTTAGCAGCCATCAATAAGGACCTGAACATGGGAGTTCTCGGCGGGATTATTGCAGGCGTTGTCGCCGC
CGTCATGTATAACCGCTTTAAAGATGCAAAGCTGCCTGACTGGCTCGGTTTCTTCGGCGGAAAACGCAGTGTTCCGATTA
TGACAGCCTTCTGTTCCATCATTCTTGCAGGCATTTTCGGCTTCGTCTGGATTTATGCACAGCAGGCAATTGACGCCATT
GGAGGATGGATCATTCATGCGGGGGCATTAGGGGTCGGGATATTCGGTCTTTTAAACAGGCTGCTTTTGCCGCTCGGCCT
TCATCACATCTTAAACAACATGGTCTGGATGGTCTTTGGAGAATTTGCAGGCAAAACGGGTGACATGAACCGGTTCTTTG
CCGGAGATCCAAGCGCCGGCGCCTTTATGACAGGGTTCTTCCCGATCATGATGTTCGGTCTTCCTGCGGCGTGTCTGGCG
ATGATTGCCGCTGCGAAAAAGCATCGCCGCAAAGCAACGGCCGGGTTGCTGATCGGATTGGCGTTCACATCATTTTTAAC
CGGAATCACAGAGCCGATTGAGTTCACATTCATGTTCCTGTCGCCGCTTCTCTTCGGTATTCATGCCTTGTTAACCGCAA
GCGCCATGACCGTGACCAATATGCTCGGCATCCATCACGGCTTTACCTTTTCAGCCGGGACGATCGACTATCTCTTAAAC
TTTGGAATCGCCACAAAGCCGCTGCTCTTGCTTCCGCTTGGTCTTGTTTACGGCATTATTTATTTCATCATCTTTTATTT
CTTGATAACGAAGCTCAATTTGAAAACGCCTGGACGAGAAGATGACGATGAGCAGGAAAACACCGCTGTCAGCCCTGACA
CAGGATCGTCTAAATACGAAACCCTTGCGGACAAATACATCGAAGCGCTTGGCGGAAAAGAAAACATCGAGACGCTGAAC
AACTGCGTGACCCGCCTCCGCCTCAAGGTCCGCGATGCTTCCAAGCTGGATGAAAGCGCGATGAAATCCATGGGAGCGAA
GGGATTCGTCAAGCTCAATCAGCACGAGGTGCAGGTGATCATCGGTACAGACGTCGAATTCTTGGCGAATGAAATGAGAA
AACGTACGGAATAA

Upstream 100 bases:

>100_bases
CGTTGACAAACGGAACGCTTGAAGTTGCCCGCCGCTGTTTATCCCATGAAAATGGACATTTGAAAAAAGCGATTCAATAT
CATCGAAAGGGGTTTTAACT

Downstream 100 bases:

>100_bases
AAGATAAGCCCCTTCTGAGGAAGGGGCTTTGTTACTTTATGATTGCTCTTTCACGTGAACTTTCAGTGTTGCTTGTACTT
CCGGATGGAGCTTCACGGGA

Product: NagP

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MLGFLQNIGRALMLPIAVLPAAGLLLALGREDLLNIPFIAASGQSILDQLPIIFAIGVAIGLSKDGHGAAALSGAIGYFV
LTGGLAAINKDLNMGVLGGIIAGVVAAVMYNRFKDAKLPDWLGFFGGKRSVPIMTAFCSIILAGIFGFVWIYAQQAIDAI
GGWIIHAGALGVGIFGLLNRLLLPLGLHHILNNMVWMVFGEFAGKTGDMNRFFAGDPSAGAFMTGFFPIMMFGLPAACLA
MIAAAKKHRRKATAGLLIGLAFTSFLTGITEPIEFTFMFLSPLLFGIHALLTASAMTVTNMLGIHHGFTFSAGTIDYLLN
FGIATKPLLLLPLGLVYGIIYFIIFYFLITKLNLKTPGREDDDEQENTAVSPDTGSSKYETLADKYIEALGGKENIETLN
NCVTRLRLKVRDASKLDESAMKSMGAKGFVKLNQHEVQVIIGTDVEFLANEMRKRTE

Sequences:

>Translated_457_residues
MLGFLQNIGRALMLPIAVLPAAGLLLALGREDLLNIPFIAASGQSILDQLPIIFAIGVAIGLSKDGHGAAALSGAIGYFV
LTGGLAAINKDLNMGVLGGIIAGVVAAVMYNRFKDAKLPDWLGFFGGKRSVPIMTAFCSIILAGIFGFVWIYAQQAIDAI
GGWIIHAGALGVGIFGLLNRLLLPLGLHHILNNMVWMVFGEFAGKTGDMNRFFAGDPSAGAFMTGFFPIMMFGLPAACLA
MIAAAKKHRRKATAGLLIGLAFTSFLTGITEPIEFTFMFLSPLLFGIHALLTASAMTVTNMLGIHHGFTFSAGTIDYLLN
FGIATKPLLLLPLGLVYGIIYFIIFYFLITKLNLKTPGREDDDEQENTAVSPDTGSSKYETLADKYIEALGGKENIETLN
NCVTRLRLKVRDASKLDESAMKSMGAKGFVKLNQHEVQVIIGTDVEFLANEMRKRTE
>Mature_457_residues
MLGFLQNIGRALMLPIAVLPAAGLLLALGREDLLNIPFIAASGQSILDQLPIIFAIGVAIGLSKDGHGAAALSGAIGYFV
LTGGLAAINKDLNMGVLGGIIAGVVAAVMYNRFKDAKLPDWLGFFGGKRSVPIMTAFCSIILAGIFGFVWIYAQQAIDAI
GGWIIHAGALGVGIFGLLNRLLLPLGLHHILNNMVWMVFGEFAGKTGDMNRFFAGDPSAGAFMTGFFPIMMFGLPAACLA
MIAAAKKHRRKATAGLLIGLAFTSFLTGITEPIEFTFMFLSPLLFGIHALLTASAMTVTNMLGIHHGFTFSAGTIDYLLN
FGIATKPLLLLPLGLVYGIIYFIIFYFLITKLNLKTPGREDDDEQENTAVSPDTGSSKYETLADKYIEALGGKENIETLN
NCVTRLRLKVRDASKLDESAMKSMGAKGFVKLNQHEVQVIIGTDVEFLANEMRKRTE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=462, Percent_Identity=52.8138528138528, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1787343, Length=475, Percent_Identity=41.2631578947368, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1787908, Length=511, Percent_Identity=34.6379647749511, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49049; Mature: 49049

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCEEEECCCEEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure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HHHCCCCCEEEECCCEEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]