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The map label for this gene is adhE [H]

Identifier: 52787967

GI number: 52787967

Start: 4137609

End: 4140212

Strand: Direct

Name: adhE [H]

Synonym: BLi04290

Alternate gene names: 52787967

Gene position: 4137609-4140212 (Clockwise)

Preceding gene: 52787966

Following gene: 52787968

Centisome position: 97.99

GC content: 49.04

Gene sequence:

>2604_bases
ATGGCAATCGAAGAAAAGAACATGAAACAAAAGCAAAATGCCTCAAACATGATTGATCAGCTAGTCGAAAAAGGCCTAAA
GGCATTGGAAGAATTCCGCAGCTTTGATCAGGAGCAGATTGATGAGATCGTCAAACAGATGGCGCTTGCCGGCCTTGATC
AGCACATGCCTCTCGCCAAACTTGCCGTCGAAGAAACGAAGCGCGGCGTTTATGAAGATAAAATCATTAAAAACATGTTT
GCCACAGAGTATGTCTACCATCATATCAAATACGATAAAACAGTGGGCATCATCAATGAAAACGAACACGACGGAGTCAT
TGAAATCGCGGAGCCTGTCGGTGTCATCGCCGGCGTGACGCCGGTGACCAACCCTACTTCCACCACCATGTTCAAGTCCC
TGATATCAATTAAAACAAGAAATCCAATCGTGTTTGCTTTCCATCCATCCGCCCAAAAATGCAGCCGCGAAGCGGCGAGA
ATCCTTCGGGACGCAGCCGTCAAAGCAGGAGCACCCGACAACTGCATCCAATGGATCGAGACGCCTTCTCTGGATGCAAC
GCAAGCCCTAATGACCCACCCGAACGTTTCCCTGATCCTCGCAACAGGAGGCGCAGGCATGGTGAAATCCGCTTACAGCT
CCGGAAAACCGGCCCTTGGCGTCGGACCTGGAAACGTTCCGTGCTACATCGAGAAGTCTGCCAACCTGAAGCAAGCCGTC
AATGATCTGATCTTATCCAAAACATTTGACAACGGCATGATTTGCGCATCAGAGCAGGCCGTCATCATAGACAAAGGCAT
CTACTCAGACGTCAAAGCCGAAATGACAAGAAACAACTGCTATTTCTTAAATAAAACCGAAAAATCAAAGGTCGAAAAAT
TGGTCATCAATGAAAATACTTGCGCGGTAAACGCTGATATCGTCGGCATGCCGGCATTCAAAATTGCTGAAATGGCCGGT
ATCAAAGTGCCGCAAGATACAAAAATCCTCATCGCCGAATTAGAAGGTGTAGGTCCGGATGACCCGCTGTCCAGAGAAAA
ACTGAGCCCTGTTTTAGCGTGCTACAAAGTCAGCGGGCTTGAAGAAGGATTAAAACGCGCCGAAGAGATGCTGGCGTTCG
GGGGAACCGGCCACTCGGCGGTGATTCATACGAACGATCAAGAAGCCGTTAAAGAATTCGGCTTAAGGATGAAGGCCGGC
CGGATCATTGTCAATGCACCGTCGTCACAAGGAGCGATCGGCGACATTTACAACGCCTATATGCCTTCTTTAACGCTCGG
CTGCGGCACATATGGCGGGAACTCCGTCTCGTCAAACGTAGGGGCCGTTCATTTAATCAACACGAAAAAAGTGGCGAAAA
GGAATGTCAATATGCAGTGGTTTAAAGTGCCTCCAAAAATCTATTTTGAAAAACACGCAACCCAATATTTAGCGAAAATG
CCTGACATCTCAAAAGCGTTTATTGTGACTGATCCGGGAATGGTGAAACTCGGATATGTAGACCGCGCCCTTCACTACTT
AAGAAGGCGCCCGGATTACGTTCATTGCGAAATTTTTTCTGATGTCGAGCCTGATCCTTCAATCGAAACGGTGATGAACG
GCGTCGACATGATGGCCAAATTCCAGCCGGATGTCATCATCGCCCTCGGCGGAGGTTCGGCAATGGATGCCGCAAAAGGA
ATGTGGATGTTCTACGAGCACCCTGATGCTGAATTCTTTGGCTTAAAGCAAAAATTTCTGGACATCCGCAAGCGCATCGT
CAAATACCCGAAACTGGGAGGAAAAGCAAAATTCGTCGCAATCCCGACGACATCAGGCACAGGTTCTGAAGTCACTTCAT
TCTCTGTCATTACAGACAAAGAAACGAATACAAAGTACCCGCTGGCCGATTATGAATTAACACCGGATGTCGCGATCATT
GACCCGCAATTTGTCATGACCGTGCCGAAACATATCACGGCCGACACAGGAATGGACGTATTGACACACGCAATTGAATC
ATACGTATCGTGCATGGCCAACGACTATACTGACGGCTTGGCCATGAAGGCCATCCAGCTGATCTTCGAATATCTGCCAA
GAGCATACAAAAACGGCAGCGACGAGCTGGCCCGCGAAAAAGTGCATAATGCATCAACGATTGCCGGAATGGCATTTTCC
AATGCCTTCCTCGGCATCAACCACAGCTTAGCCCATAAGCTTGGCGCCGAGTTTCAGATCGCTCATGGACGGGCAAACGC
CATCCTTCTGCCGCACGTGATCCGCTATAATGCAGCGAAGCCGAAAAAGTTCACGGCATTTCCTAAATACAGCCACTTCA
TAGCCGACCAGCGCTACGCGGAGATTGCCAGAACGCTCGGCCTGCCTGCAAAAACAACTGCAGAAGGTGTTGAAAGCCTC
ATCCAGGAGATCATCTCGCTTGCCAAAGAGCTTAAAATTCCGATGAGCATTAAACAAAACGGCGTGGATGCCGCCGCATT
CGAAAGCAAAGTAGATCTCATGGCCGAGCGCGCCTTTGAAGATCAGTGCACGACAGCCAATCCTAAGCTGCCGCTCGTTT
CAGAGCTGGCTGAGATTTACCGTTCCGCGTATAAAGGAGTTTAG

Upstream 100 bases:

>100_bases
TAACCTAACTTGTGAAATGTTTCACAAACTTTACCGACTTCATTCATCCCTGGACATCCTGTTGTAATCAAATTCCTATT
CATTCACTGGAGGGTTTACA

Downstream 100 bases:

>100_bases
CCAAAAAAGCCGTCATTCCGACGGCTTTAGTTTGTTGACAAAATCCCAAAACGACTTTTAGTTTTGGGATTTTGTCATCC
TTTCAGCGTGATTGAAAACC

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 867; Mature: 866

Protein sequence:

>867_residues
MAIEEKNMKQKQNASNMIDQLVEKGLKALEEFRSFDQEQIDEIVKQMALAGLDQHMPLAKLAVEETKRGVYEDKIIKNMF
ATEYVYHHIKYDKTVGIINENEHDGVIEIAEPVGVIAGVTPVTNPTSTTMFKSLISIKTRNPIVFAFHPSAQKCSREAAR
ILRDAAVKAGAPDNCIQWIETPSLDATQALMTHPNVSLILATGGAGMVKSAYSSGKPALGVGPGNVPCYIEKSANLKQAV
NDLILSKTFDNGMICASEQAVIIDKGIYSDVKAEMTRNNCYFLNKTEKSKVEKLVINENTCAVNADIVGMPAFKIAEMAG
IKVPQDTKILIAELEGVGPDDPLSREKLSPVLACYKVSGLEEGLKRAEEMLAFGGTGHSAVIHTNDQEAVKEFGLRMKAG
RIIVNAPSSQGAIGDIYNAYMPSLTLGCGTYGGNSVSSNVGAVHLINTKKVAKRNVNMQWFKVPPKIYFEKHATQYLAKM
PDISKAFIVTDPGMVKLGYVDRALHYLRRRPDYVHCEIFSDVEPDPSIETVMNGVDMMAKFQPDVIIALGGGSAMDAAKG
MWMFYEHPDAEFFGLKQKFLDIRKRIVKYPKLGGKAKFVAIPTTSGTGSEVTSFSVITDKETNTKYPLADYELTPDVAII
DPQFVMTVPKHITADTGMDVLTHAIESYVSCMANDYTDGLAMKAIQLIFEYLPRAYKNGSDELAREKVHNASTIAGMAFS
NAFLGINHSLAHKLGAEFQIAHGRANAILLPHVIRYNAAKPKKFTAFPKYSHFIADQRYAEIARTLGLPAKTTAEGVESL
IQEIISLAKELKIPMSIKQNGVDAAAFESKVDLMAERAFEDQCTTANPKLPLVSELAEIYRSAYKGV

Sequences:

>Translated_867_residues
MAIEEKNMKQKQNASNMIDQLVEKGLKALEEFRSFDQEQIDEIVKQMALAGLDQHMPLAKLAVEETKRGVYEDKIIKNMF
ATEYVYHHIKYDKTVGIINENEHDGVIEIAEPVGVIAGVTPVTNPTSTTMFKSLISIKTRNPIVFAFHPSAQKCSREAAR
ILRDAAVKAGAPDNCIQWIETPSLDATQALMTHPNVSLILATGGAGMVKSAYSSGKPALGVGPGNVPCYIEKSANLKQAV
NDLILSKTFDNGMICASEQAVIIDKGIYSDVKAEMTRNNCYFLNKTEKSKVEKLVINENTCAVNADIVGMPAFKIAEMAG
IKVPQDTKILIAELEGVGPDDPLSREKLSPVLACYKVSGLEEGLKRAEEMLAFGGTGHSAVIHTNDQEAVKEFGLRMKAG
RIIVNAPSSQGAIGDIYNAYMPSLTLGCGTYGGNSVSSNVGAVHLINTKKVAKRNVNMQWFKVPPKIYFEKHATQYLAKM
PDISKAFIVTDPGMVKLGYVDRALHYLRRRPDYVHCEIFSDVEPDPSIETVMNGVDMMAKFQPDVIIALGGGSAMDAAKG
MWMFYEHPDAEFFGLKQKFLDIRKRIVKYPKLGGKAKFVAIPTTSGTGSEVTSFSVITDKETNTKYPLADYELTPDVAII
DPQFVMTVPKHITADTGMDVLTHAIESYVSCMANDYTDGLAMKAIQLIFEYLPRAYKNGSDELAREKVHNASTIAGMAFS
NAFLGINHSLAHKLGAEFQIAHGRANAILLPHVIRYNAAKPKKFTAFPKYSHFIADQRYAEIARTLGLPAKTTAEGVESL
IQEIISLAKELKIPMSIKQNGVDAAAFESKVDLMAERAFEDQCTTANPKLPLVSELAEIYRSAYKGV
>Mature_866_residues
AIEEKNMKQKQNASNMIDQLVEKGLKALEEFRSFDQEQIDEIVKQMALAGLDQHMPLAKLAVEETKRGVYEDKIIKNMFA
TEYVYHHIKYDKTVGIINENEHDGVIEIAEPVGVIAGVTPVTNPTSTTMFKSLISIKTRNPIVFAFHPSAQKCSREAARI
LRDAAVKAGAPDNCIQWIETPSLDATQALMTHPNVSLILATGGAGMVKSAYSSGKPALGVGPGNVPCYIEKSANLKQAVN
DLILSKTFDNGMICASEQAVIIDKGIYSDVKAEMTRNNCYFLNKTEKSKVEKLVINENTCAVNADIVGMPAFKIAEMAGI
KVPQDTKILIAELEGVGPDDPLSREKLSPVLACYKVSGLEEGLKRAEEMLAFGGTGHSAVIHTNDQEAVKEFGLRMKAGR
IIVNAPSSQGAIGDIYNAYMPSLTLGCGTYGGNSVSSNVGAVHLINTKKVAKRNVNMQWFKVPPKIYFEKHATQYLAKMP
DISKAFIVTDPGMVKLGYVDRALHYLRRRPDYVHCEIFSDVEPDPSIETVMNGVDMMAKFQPDVIIALGGGSAMDAAKGM
WMFYEHPDAEFFGLKQKFLDIRKRIVKYPKLGGKAKFVAIPTTSGTGSEVTSFSVITDKETNTKYPLADYELTPDVAIID
PQFVMTVPKHITADTGMDVLTHAIESYVSCMANDYTDGLAMKAIQLIFEYLPRAYKNGSDELAREKVHNASTIAGMAFSN
AFLGINHSLAHKLGAEFQIAHGRANAILLPHVIRYNAAKPKKFTAFPKYSHFIADQRYAEIARTLGLPAKTTAEGVESLI
QEIISLAKELKIPMSIKQNGVDAAAFESKVDLMAERAFEDQCTTANPKLPLVSELAEIYRSAYKGV

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=327, Percent_Identity=26.2996941896024, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1787493, Length=861, Percent_Identity=50.9872241579559, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=386, Percent_Identity=35.4922279792746, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI87082107, Length=339, Percent_Identity=35.9882005899705, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1789163, Length=382, Percent_Identity=34.0314136125654, Blast_Score=195, Evalue=1e-50,
Organism=Escherichia coli, GI1788797, Length=385, Percent_Identity=28.051948051948, Blast_Score=154, Evalue=3e-38,
Organism=Escherichia coli, GI1789386, Length=394, Percent_Identity=24.1116751269036, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537053, Length=348, Percent_Identity=25, Blast_Score=112, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321181, Length=410, Percent_Identity=34.8780487804878, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24657991, Length=326, Percent_Identity=28.2208588957055, Blast_Score=95, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 95009; Mature: 94877

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEEKNMKQKQNASNMIDQLVEKGLKALEEFRSFDQEQIDEIVKQMALAGLDQHMPLAK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETKRGVYEDKIIKNMFATEYVYHHIKYDKTVGIINENEHDGVIEIAEPVGVIAGVT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCEEEECCCHHHHCCCC
PVTNPTSTTMFKSLISIKTRNPIVFAFHPSAQKCSREAARILRDAAVKAGAPDNCIQWIE
CCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
TPSLDATQALMTHPNVSLILATGGAGMVKSAYSSGKPALGVGPGNVPCYIEKSANLKQAV
CCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCEEECCCCCCCEEEECCCCHHHHH
NDLILSKTFDNGMICASEQAVIIDKGIYSDVKAEMTRNNCYFLNKTEKSKVEKLVINENT
HHHHHHHCCCCCEEEECCCEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHEEEECCCC
CAVNADIVGMPAFKIAEMAGIKVPQDTKILIAELEGVGPDDPLSREKLSPVLACYKVSGL
EEEECCEECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
EEGLKRAEEMLAFGGTGHSAVIHTNDQEAVKEFGLRMKAGRIIVNAPSSQGAIGDIYNAY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCEEECCEEEEECCCCCCCHHHHHHHH
MPSLTLGCGTYGGNSVSSNVGAVHLINTKKVAKRNVNMQWFKVPPKIYFEKHATQYLAKM
CCCEEEECCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHC
PDISKAFIVTDPGMVKLGYVDRALHYLRRRPDYVHCEIFSDVEPDPSIETVMNGVDMMAK
CCCCCEEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
FQPDVIIALGGGSAMDAAKGMWMFYEHPDAEFFGLKQKFLDIRKRIVKYPKLGGKAKFVA
CCCCEEEEECCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IPTTSGTGSEVTSFSVITDKETNTKYPLADYELTPDVAIIDPQFVMTVPKHITADTGMDV
EECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCHHEEECCCHHCCCCCHHH
LTHAIESYVSCMANDYTDGLAMKAIQLIFEYLPRAYKNGSDELAREKVHNASTIAGMAFS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
NAFLGINHSLAHKLGAEFQIAHGRANAILLPHVIRYNAAKPKKFTAFPKYSHFIADQRYA
CHHCCCCHHHHHHCCCCEEEECCCCCEEECCHHHHCCCCCCCCEECCCCHHHHHHHHHHH
EIARTLGLPAKTTAEGVESLIQEIISLAKELKIPMSIKQNGVDAAAFESKVDLMAERAFE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH
DQCTTANPKLPLVSELAEIYRSAYKGV
CCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIEEKNMKQKQNASNMIDQLVEKGLKALEEFRSFDQEQIDEIVKQMALAGLDQHMPLAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETKRGVYEDKIIKNMFATEYVYHHIKYDKTVGIINENEHDGVIEIAEPVGVIAGVT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCEEEECCCHHHHCCCC
PVTNPTSTTMFKSLISIKTRNPIVFAFHPSAQKCSREAARILRDAAVKAGAPDNCIQWIE
CCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
TPSLDATQALMTHPNVSLILATGGAGMVKSAYSSGKPALGVGPGNVPCYIEKSANLKQAV
CCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCEEECCCCCCCEEEECCCCHHHHH
NDLILSKTFDNGMICASEQAVIIDKGIYSDVKAEMTRNNCYFLNKTEKSKVEKLVINENT
HHHHHHHCCCCCEEEECCCEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHEEEECCCC
CAVNADIVGMPAFKIAEMAGIKVPQDTKILIAELEGVGPDDPLSREKLSPVLACYKVSGL
EEEECCEECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
EEGLKRAEEMLAFGGTGHSAVIHTNDQEAVKEFGLRMKAGRIIVNAPSSQGAIGDIYNAY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCEEECCEEEEECCCCCCCHHHHHHHH
MPSLTLGCGTYGGNSVSSNVGAVHLINTKKVAKRNVNMQWFKVPPKIYFEKHATQYLAKM
CCCEEEECCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHC
PDISKAFIVTDPGMVKLGYVDRALHYLRRRPDYVHCEIFSDVEPDPSIETVMNGVDMMAK
CCCCCEEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
FQPDVIIALGGGSAMDAAKGMWMFYEHPDAEFFGLKQKFLDIRKRIVKYPKLGGKAKFVA
CCCCEEEEECCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IPTTSGTGSEVTSFSVITDKETNTKYPLADYELTPDVAIIDPQFVMTVPKHITADTGMDV
EECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCHHEEECCCHHCCCCCHHH
LTHAIESYVSCMANDYTDGLAMKAIQLIFEYLPRAYKNGSDELAREKVHNASTIAGMAFS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
NAFLGINHSLAHKLGAEFQIAHGRANAILLPHVIRYNAAKPKKFTAFPKYSHFIADQRYA
CHHCCCCHHHHHHCCCCEEEECCCCCEEECCHHHHCCCCCCCCEECCCCHHHHHHHHHHH
EIARTLGLPAKTTAEGVESLIQEIISLAKELKIPMSIKQNGVDAAAFESKVDLMAERAFE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH
DQCTTANPKLPLVSELAEIYRSAYKGV
CCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]