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The map label for this gene is ald [H]

Identifier: 52787952

GI number: 52787952

Start: 4117470

End: 4118600

Strand: Direct

Name: ald [H]

Synonym: BLi04275

Alternate gene names: 52787952

Gene position: 4117470-4118600 (Clockwise)

Preceding gene: 52787949

Following gene: 52787959

Centisome position: 97.51

GC content: 51.55

Gene sequence:

>1131_bases
ATGATCATTGGAATTCCTAAAGAAATTAAAAACAACGAGAACCGTGTAGCCATTACTCCGGCCGGAGTAGTCGCGCTAAA
AAAAAGCGGTCATCAAGTTTTAATTGAACAGGGAGCCGGGCTTGGAAGCGGATTCGAGGACGCCGACTACAAAGCAGCCG
GGGCGGACATTTTGGCTGAAGCGAAAGATGTGTGGGGAAAAGCGGATATGGTCATGAAAGTGAAAGAGCCGATCAGCTCT
GAATACGGCTATTTCCGAAAAGGACTTGTTTTATTTACTTATCTGCACTTAGCGGCTGAACCTAAGCTTGCGAAAGCGCT
TGTGGACAGCGGCGTCACAGCCATCGCTTACGAAACGGTCGAAGTGAACCGGACGCTTCCGCTCTTGACTCCAATGAGTG
AAGTCGCCGGACGCATGGCTTCGCAGATCGGCGCGCAATTTCTTGAGAAATCAAAAGGCGGCAAAGGCATCCTGTTATCG
GGCGTACCCGGTGTAAAACGCGGCAAAGTCACGATCATCGGCGGCGGAGTGGTTGGTACGAACGCTGCCAAAATCGCTGT
CGGACTTGGCGCTGACGTCACTTTGATCGACTTGAGCGCTGAGCGCCTCCGTCAGCTCGATGACCAGTTCGGCAAAGACA
TTCAAACGCTTATGTCCAATCCGCTCAACATTGCGGAAGCCGTAAAAGACTCAGACCTTGTCATCGGCGCCGTCTTAATT
CCTGGTGCAAAAGCGCCGAAGCTTGTCACAGAAGAGATGATCAAGTCGATGTCGCCAGGTTCAGTTGTTGTAGACGTGGC
GATTGACCAGGGCGGCATTATCGAAACGGTTGATAAAATCACTACGCATGACGATCCGACTTACACGAAGCACGGCGTTG
TCCACTATGCCGTCGCCAATATGCCGGGAGCCGTTCCCCGTACATCAACGATCGGCTTAACAAACGTCACCATGCCTTAC
GCCTTGCAAATCGCAAATAAAGGCGTTGAACAAGCAGTCGCCGAAAATCAGGCGCTCGCTCTCGGCGTCAACGTTGCAAA
CGGAAGCATCACCTACAAGGCCGTCGCGCGCGACCTTGGATATGAATATGTATCTGTAGAAGATGCATTTCAAACGGTAA
CCGCTGGTTAA

Upstream 100 bases:

>100_bases
AAAAAAGCGCTTACATTTTTAATTTTACACACGATGCTCTTCCCGATTCAGAGATTTATACTAGCGGTAGAATCATATTT
CATTTGAGGGGGACGACATC

Downstream 100 bases:

>100_bases
GCGGATTTTATCTCTTGAGAACATTTTAACAACTCTTTCTGACTGTCCTTTCACGAAAAAAGGCTGGATGATATCCGGCC
TTTTTTCCATTCTGCTTTAA

Product: hypothetical protein

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIIGIPKEIKNNENRVAITPAGVVALKKSGHQVLIEQGAGLGSGFEDADYKAAGADILAEAKDVWGKADMVMKVKEPISS
EYGYFRKGLVLFTYLHLAAEPKLAKALVDSGVTAIAYETVEVNRTLPLLTPMSEVAGRMASQIGAQFLEKSKGGKGILLS
GVPGVKRGKVTIIGGGVVGTNAAKIAVGLGADVTLIDLSAERLRQLDDQFGKDIQTLMSNPLNIAEAVKDSDLVIGAVLI
PGAKAPKLVTEEMIKSMSPGSVVVDVAIDQGGIIETVDKITTHDDPTYTKHGVVHYAVANMPGAVPRTSTIGLTNVTMPY
ALQIANKGVEQAVAENQALALGVNVANGSITYKAVARDLGYEYVSVEDAFQTVTAG

Sequences:

>Translated_376_residues
MIIGIPKEIKNNENRVAITPAGVVALKKSGHQVLIEQGAGLGSGFEDADYKAAGADILAEAKDVWGKADMVMKVKEPISS
EYGYFRKGLVLFTYLHLAAEPKLAKALVDSGVTAIAYETVEVNRTLPLLTPMSEVAGRMASQIGAQFLEKSKGGKGILLS
GVPGVKRGKVTIIGGGVVGTNAAKIAVGLGADVTLIDLSAERLRQLDDQFGKDIQTLMSNPLNIAEAVKDSDLVIGAVLI
PGAKAPKLVTEEMIKSMSPGSVVVDVAIDQGGIIETVDKITTHDDPTYTKHGVVHYAVANMPGAVPRTSTIGLTNVTMPY
ALQIANKGVEQAVAENQALALGVNVANGSITYKAVARDLGYEYVSVEDAFQTVTAG
>Mature_376_residues
MIIGIPKEIKNNENRVAITPAGVVALKKSGHQVLIEQGAGLGSGFEDADYKAAGADILAEAKDVWGKADMVMKVKEPISS
EYGYFRKGLVLFTYLHLAAEPKLAKALVDSGVTAIAYETVEVNRTLPLLTPMSEVAGRMASQIGAQFLEKSKGGKGILLS
GVPGVKRGKVTIIGGGVVGTNAAKIAVGLGADVTLIDLSAERLRQLDDQFGKDIQTLMSNPLNIAEAVKDSDLVIGAVLI
PGAKAPKLVTEEMIKSMSPGSVVVDVAIDQGGIIETVDKITTHDDPTYTKHGVVHYAVANMPGAVPRTSTIGLTNVTMPY
ALQIANKGVEQAVAENQALALGVNVANGSITYKAVARDLGYEYVSVEDAFQTVTAG

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=355, Percent_Identity=31.2676056338028, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI122939153, Length=355, Percent_Identity=31.2676056338028, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787887, Length=379, Percent_Identity=31.6622691292876, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550456, Length=345, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39338; Mature: 39338

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGIPKEIKNNENRVAITPAGVVALKKSGHQVLIEQGAGLGSGFEDADYKAAGADILAE
CEECCCHHHCCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHH
AKDVWGKADMVMKVKEPISSEYGYFRKGLVLFTYLHLAAEPKLAKALVDSGVTAIAYETV
HHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEE
EVNRTLPLLTPMSEVAGRMASQIGAQFLEKSKGGKGILLSGVPGVKRGKVTIIGGGVVGT
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKIAVGLGADVTLIDLSAERLRQLDDQFGKDIQTLMSNPLNIAEAVKDSDLVIGAVLI
CCEEEEEECCCCEEEEECCHHHHHHHHHHHCHHHHHHHCCCCCHHHHHCCCCEEEEEEEE
PGAKAPKLVTEEMIKSMSPGSVVVDVAIDQGGIIETVDKITTHDDPTYTKHGVVHYAVAN
CCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECC
MPGAVPRTSTIGLTNVTMPYALQIANKGVEQAVAENQALALGVNVANGSITYKAVARDLG
CCCCCCCCCCCEEEECCCHHHHHHHHCCHHHHHHCCCEEEEEEEECCCCEEHHHHHHHHC
YEYVSVEDAFQTVTAG
CEEEEHHHHHHHHCCC
>Mature Secondary Structure
MIIGIPKEIKNNENRVAITPAGVVALKKSGHQVLIEQGAGLGSGFEDADYKAAGADILAE
CEECCCHHHCCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHH
AKDVWGKADMVMKVKEPISSEYGYFRKGLVLFTYLHLAAEPKLAKALVDSGVTAIAYETV
HHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEE
EVNRTLPLLTPMSEVAGRMASQIGAQFLEKSKGGKGILLSGVPGVKRGKVTIIGGGVVGT
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKIAVGLGADVTLIDLSAERLRQLDDQFGKDIQTLMSNPLNIAEAVKDSDLVIGAVLI
CCEEEEEECCCCEEEEECCHHHHHHHHHHHCHHHHHHHCCCCCHHHHHCCCCEEEEEEEE
PGAKAPKLVTEEMIKSMSPGSVVVDVAIDQGGIIETVDKITTHDDPTYTKHGVVHYAVAN
CCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECC
MPGAVPRTSTIGLTNVTMPYALQIANKGVEQAVAENQALALGVNVANGSITYKAVARDLG
CCCCCCCCCCCEEEECCCHHHHHHHHCCHHHHHHCCCEEEEEEEECCCCEEHHHHHHHHC
YEYVSVEDAFQTVTAG
CEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]