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The map label for this gene is ywcJ [H]

Identifier: 52787812

GI number: 52787812

Start: 3962146

End: 3962934

Strand: Direct

Name: ywcJ [H]

Synonym: BLi04135

Alternate gene names: 52787812

Gene position: 3962146-3962934 (Clockwise)

Preceding gene: 52787806

Following gene: 52787813

Centisome position: 93.83

GC content: 45.75

Gene sequence:

>789_bases
ATGGAATCTAAATCACTTGTTCTAGTCGAAGAGCTTGCTTTAAAAAAGCAAAGCATCTTTGTGCAAAGCCCGCTTCGCTA
TCTTTTACGGGCTATGCTGGCCAGTATGTTTATCGGGTTCGGAGTCATTGTAGCGTTTAAAACGGGGAATCTCTTTTATG
TTGAACATTCTCCGTTCACATATCCAATTGCAGCAATCACCTTTGGGGGCGCCATTATTCTGATCGTCTATGGCGGAGGA
GATTTATTTACGGGAAATACATTTTATTATACATTGGCTGCGCTTCGTAAAAAAATGCGCTGGCCCACTGTGATCAAGCT
GTGGATGTTCAGCTATGCAGGAAATTTAATGGGCGCCGTTTTTTTCGCAGGCCTCATCTTTGCGACCGGCTTATTTAAAG
ATCCTTCTTCCACCGGCTTTATTCTCAGCGTGGCGGAGCATAAAATGAATACGCCGGTGACCGAATTGTTCTTTAAAGCG
ATTCTGTGTAACTGGCTTGTCTGCCTTGCGTTTTTCGTTCCGATGGCGCAAAAAGGGGACGGAGCGAAAATGTTCTCTAT
GATGCTGTTTGTCTTTACTTTCTTTATTTCCGGTTATGAACACAGCATCGCGAACATGGCCACTTTTGCCGTCTCTCTGG
TCACCGAGCATCCGGAGACCATTTCGATTGCAGGCGCAATCCATAACCTAATTCCGGTAACACTCGGGAACCTGGTCGGA
GGCGCGTTTATGATGGGCTTCATGTACCACTATGCCAACAAGCCGGGCGGTGAAGAAATCATCAAATAA

Upstream 100 bases:

>100_bases
AATACACAAAATTTGTGAAATGTTTCACAATATCCTGCTATACTTAGGTTGTATTTATGATAGATACTTCTTAAGTAAAC
ACTTGATGGTGGTGGAAAAC

Downstream 100 bases:

>100_bases
AAAGATCGTCCATTTTTAATGGATTCTAAATATGCGGGCTCCCTTCATAAAATCGTGGTAACTTTAAAAATGGAGGTTCA
GCGATGCAAAGTTTTCATTC

Product: YwcJ

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MESKSLVLVEELALKKQSIFVQSPLRYLLRAMLASMFIGFGVIVAFKTGNLFYVEHSPFTYPIAAITFGGAIILIVYGGG
DLFTGNTFYYTLAALRKKMRWPTVIKLWMFSYAGNLMGAVFFAGLIFATGLFKDPSSTGFILSVAEHKMNTPVTELFFKA
ILCNWLVCLAFFVPMAQKGDGAKMFSMMLFVFTFFISGYEHSIANMATFAVSLVTEHPETISIAGAIHNLIPVTLGNLVG
GAFMMGFMYHYANKPGGEEIIK

Sequences:

>Translated_262_residues
MESKSLVLVEELALKKQSIFVQSPLRYLLRAMLASMFIGFGVIVAFKTGNLFYVEHSPFTYPIAAITFGGAIILIVYGGG
DLFTGNTFYYTLAALRKKMRWPTVIKLWMFSYAGNLMGAVFFAGLIFATGLFKDPSSTGFILSVAEHKMNTPVTELFFKA
ILCNWLVCLAFFVPMAQKGDGAKMFSMMLFVFTFFISGYEHSIANMATFAVSLVTEHPETISIAGAIHNLIPVTLGNLVG
GAFMMGFMYHYANKPGGEEIIK
>Mature_262_residues
MESKSLVLVEELALKKQSIFVQSPLRYLLRAMLASMFIGFGVIVAFKTGNLFYVEHSPFTYPIAAITFGGAIILIVYGGG
DLFTGNTFYYTLAALRKKMRWPTVIKLWMFSYAGNLMGAVFFAGLIFATGLFKDPSSTGFILSVAEHKMNTPVTELFFKA
ILCNWLVCLAFFVPMAQKGDGAKMFSMMLFVFTFFISGYEHSIANMATFAVSLVTEHPETISIAGAIHNLIPVTLGNLVG
GAFMMGFMYHYANKPGGEEIIK

Specific function: Potential Nitrite Transporter. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=255, Percent_Identity=36.4705882352941, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1787132, Length=267, Percent_Identity=31.0861423220974, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788837, Length=273, Percent_Identity=26.7399267399267, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321779, Length=186, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 28920; Mature: 28920

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKSLVLVEELALKKQSIFVQSPLRYLLRAMLASMFIGFGVIVAFKTGNLFYVEHSPFT
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCC
YPIAAITFGGAIILIVYGGGDLFTGNTFYYTLAALRKKMRWPTVIKLWMFSYAGNLMGAV
CHHHHHHHCCEEEEEEECCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FFAGLIFATGLFKDPSSTGFILSVAEHKMNTPVTELFFKAILCNWLVCLAFFVPMAQKGD
HHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAKMFSMMLFVFTFFISGYEHSIANMATFAVSLVTEHPETISIAGAIHNLIPVTLGNLVG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
GAFMMGFMYHYANKPGGEEIIK
HHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MESKSLVLVEELALKKQSIFVQSPLRYLLRAMLASMFIGFGVIVAFKTGNLFYVEHSPFT
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCC
YPIAAITFGGAIILIVYGGGDLFTGNTFYYTLAALRKKMRWPTVIKLWMFSYAGNLMGAV
CHHHHHHHCCEEEEEEECCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FFAGLIFATGLFKDPSSTGFILSVAEHKMNTPVTELFFKAILCNWLVCLAFFVPMAQKGD
HHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAKMFSMMLFVFTFFISGYEHSIANMATFAVSLVTEHPETISIAGAIHNLIPVTLGNLVG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
GAFMMGFMYHYANKPGGEEIIK
HHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]