Click here to switch to the map view.

The map label for this gene is dgoD [H]

Identifier: 52787747

GI number: 52787747

Start: 3895067

End: 3896215

Strand: Reverse

Name: dgoD [H]

Synonym: BLi04070

Alternate gene names: 52787747

Gene position: 3896215-3895067 (Counterclockwise)

Preceding gene: 52787748

Following gene: 52787746

Centisome position: 92.27

GC content: 50.83

Gene sequence:

>1149_bases
ATGAAAATAACAGGATTCGAGTGTTTCCTAATTCCGCCGCGCTGGCTTTTCTTAAAAATCGAAACGGATGAAGGCATTAC
AGGATGGGGAGAGCCGGTAATCGAGGGAAAGGCCGCGACAGTTAAGGCGGCTGTGGGCGAGTTAATGGAATATTTGATCG
GCAAAGACCCGATGAACATCGAAGACCATTGGAATGTCATGTACAGAGGCGGGTTTTATCGGGGCGGGCCCATTCTGATG
AGCGCGATTGCAGGCATCGATCAAGCGCTTTGGGACATTAAGGGCAAGTTTTACAACGCTCCCGTGCATCAGCTGCTCGG
AGGGAAGAACAGGGAATCGATCAAGGTCTATTCATGGATTGGCGGAGACAGGCCTCAAGACGTCGGCCTCGCTGCCAAAC
AGGTGGTTGATAAAGGCTTTACAGCCGTCAAAATGAACGGCACGGAAGAGCTGCAATACATCGATTCACATGAAAAAATC
GATCAGGTGATTGAAAGAATATCAGCAGTCAGAGAAGCGGTCGGCCCGTACATCGGAATCGGCATTGACTTTCACGGCAG
GGTGCACAAGCCGATGGCGAAGATCTTGGCAAAGGAGCTTGAACCGTTTCGGCCGATGTTTATAGAAGAGCCTGTTCTGC
CGGAAAACAACGAGGCGCTCCGCGATATCGCCAGCCTCGTCTCAATTCCGATTGCGACTGGAGAACGGATGTTTTCAAAG
TGGCAGTTTAAAAACCTGCTGACTGACGGCTATGTCGATATCATTCAGCCTGACTTGTCACACGCCGGCGGAATTACCGA
ATGCAAAAAGATCCTGTCGATGGCGGAAGCATTTGATGTCGCCGCCGCCCCTCATTGTCCGCTGGGCCCGATCGCTCTGG
CGGCATGCCTTCAGGTTGATGCAACGTGCCACAATGCATTTATACAGGAACAAAGCTTAGGGATCCATTACAACAAAGGG
ACGGATTTGCTTGATTATATAGTAGATCAAAAGGTGTTTTTCTATGAGGACGGCTATGTCCGGATTCCGGATGGACCTGG
CCTTGGGGTGGACATCAATGAAGATCACGTCCGTAAAATGGCTGATATCGGGCACAACTGGCGCAATCCGGTATGGCGGC
ACAAGGACGGAAGCATAGCTGAATGGTAA

Upstream 100 bases:

>100_bases
CGGGCTAATAAAGATGTTAATGACGCGTTTTTAGAAGAGCTGTCCAAAAAAGCAAAGCAATTTGTTGAAGCAGCAAAACA
GTAAAAAGGGGAGATCAGAG

Downstream 100 bases:

>100_bases
AAATGATACCGTTTTCAAAAGGTGTCCGTCATCACAAACAAATAAAAGGAGAGGGCCTGTCATGAAACAAACAACTTTAA
AAGAGAAGCCGACAAAGGTT

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITGFECFLIPPRWLFLKIETDEGITGWGEPVIEGKAATVKAAVGELMEYLIGKDPMNIEDHWNVMYRGGFYRGGPILM
SAIAGIDQALWDIKGKFYNAPVHQLLGGKNRESIKVYSWIGGDRPQDVGLAAKQVVDKGFTAVKMNGTEELQYIDSHEKI
DQVIERISAVREAVGPYIGIGIDFHGRVHKPMAKILAKELEPFRPMFIEEPVLPENNEALRDIASLVSIPIATGERMFSK
WQFKNLLTDGYVDIIQPDLSHAGGITECKKILSMAEAFDVAAAPHCPLGPIALAACLQVDATCHNAFIQEQSLGIHYNKG
TDLLDYIVDQKVFFYEDGYVRIPDGPGLGVDINEDHVRKMADIGHNWRNPVWRHKDGSIAEW

Sequences:

>Translated_382_residues
MKITGFECFLIPPRWLFLKIETDEGITGWGEPVIEGKAATVKAAVGELMEYLIGKDPMNIEDHWNVMYRGGFYRGGPILM
SAIAGIDQALWDIKGKFYNAPVHQLLGGKNRESIKVYSWIGGDRPQDVGLAAKQVVDKGFTAVKMNGTEELQYIDSHEKI
DQVIERISAVREAVGPYIGIGIDFHGRVHKPMAKILAKELEPFRPMFIEEPVLPENNEALRDIASLVSIPIATGERMFSK
WQFKNLLTDGYVDIIQPDLSHAGGITECKKILSMAEAFDVAAAPHCPLGPIALAACLQVDATCHNAFIQEQSLGIHYNKG
TDLLDYIVDQKVFFYEDGYVRIPDGPGLGVDINEDHVRKMADIGHNWRNPVWRHKDGSIAEW
>Mature_382_residues
MKITGFECFLIPPRWLFLKIETDEGITGWGEPVIEGKAATVKAAVGELMEYLIGKDPMNIEDHWNVMYRGGFYRGGPILM
SAIAGIDQALWDIKGKFYNAPVHQLLGGKNRESIKVYSWIGGDRPQDVGLAAKQVVDKGFTAVKMNGTEELQYIDSHEKI
DQVIERISAVREAVGPYIGIGIDFHGRVHKPMAKILAKELEPFRPMFIEEPVLPENNEALRDIASLVSIPIATGERMFSK
WQFKNLLTDGYVDIIQPDLSHAGGITECKKILSMAEAFDVAAAPHCPLGPIALAACLQVDATCHNAFIQEQSLGIHYNKG
TDLLDYIVDQKVFFYEDGYVRIPDGPGLGVDINEDHVRKMADIGHNWRNPVWRHKDGSIAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=59.9476439790576, Blast_Score=479, Evalue=1e-137,
Organism=Escherichia coli, GI1787864, Length=413, Percent_Identity=32.2033898305085, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI226510960, Length=274, Percent_Identity=27.3722627737226, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42614; Mature: 42614

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITGFECFLIPPRWLFLKIETDEGITGWGEPVIEGKAATVKAAVGELMEYLIGKDPMNI
CCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
EDHWNVMYRGGFYRGGPILMSAIAGIDQALWDIKGKFYNAPVHQLLGGKNRESIKVYSWI
CCCCEEEECCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEEC
GGDRPQDVGLAAKQVVDKGFTAVKMNGTEELQYIDSHEKIDQVIERISAVREAVGPYIGI
CCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GIDFHGRVHKPMAKILAKELEPFRPMFIEEPVLPENNEALRDIASLVSIPIATGERMFSK
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
WQFKNLLTDGYVDIIQPDLSHAGGITECKKILSMAEAFDVAAAPHCPLGPIALAACLQVD
HHHHHHHCCCCHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ATCHNAFIQEQSLGIHYNKGTDLLDYIVDQKVFFYEDGYVRIPDGPGLGVDINEDHVRKM
HHHHHHHHHHCCCCEEECCCCHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCHHHHHHH
ADIGHNWRNPVWRHKDGSIAEW
HHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITGFECFLIPPRWLFLKIETDEGITGWGEPVIEGKAATVKAAVGELMEYLIGKDPMNI
CCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
EDHWNVMYRGGFYRGGPILMSAIAGIDQALWDIKGKFYNAPVHQLLGGKNRESIKVYSWI
CCCCEEEECCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEEC
GGDRPQDVGLAAKQVVDKGFTAVKMNGTEELQYIDSHEKIDQVIERISAVREAVGPYIGI
CCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GIDFHGRVHKPMAKILAKELEPFRPMFIEEPVLPENNEALRDIASLVSIPIATGERMFSK
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
WQFKNLLTDGYVDIIQPDLSHAGGITECKKILSMAEAFDVAAAPHCPLGPIALAACLQVD
HHHHHHHCCCCHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ATCHNAFIQEQSLGIHYNKGTDLLDYIVDQKVFFYEDGYVRIPDGPGLGVDINEDHVRKM
HHHHHHHHHHCCCCEEECCCCHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCHHHHHHH
ADIGHNWRNPVWRHKDGSIAEW
HHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA