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The map label for this gene is sacP [H]

Identifier: 52787694

GI number: 52787694

Start: 3839792

End: 3841171

Strand: Reverse

Name: sacP [H]

Synonym: BLi04017

Alternate gene names: 52787694

Gene position: 3841171-3839792 (Counterclockwise)

Preceding gene: 52787695

Following gene: 52787693

Centisome position: 90.97

GC content: 46.45

Gene sequence:

>1380_bases
ATGATTCATAAAGAAATCGCTGTACGTCTTATACAGCTTCTTGGCGGAGAAGATAATGTCATCAGTGCAGCTCATTGTGC
GACAAGGCTTCGTTTGGTGATTAAGGACGAAGCAAAAATCGACCAGGAAAAGGTCGAAGAGCTGGAAGGTGTAAAAGGAG
CCTTTTCAAGCTCTGGTCAATACCAGATTATTTTCGGAACAGGGCTTGTTAATAAAGTGTATGAACAGTTTGCCAAAGAG
CTGAATCTTGACCAAAAGGAAACGGTTCAGCACAGCGATGCCGCAAAGGGAAAAATGAATCCTTTAGCGCGATTTGCGAA
AACGCTGTCAAATATTTTCGTGCCAATCATCCCGGCCATTGTTGCAAGCGGTTTATTAATGGGGCTGTTGGGAATGATGA
AGGCATTTCAGTGGGTCGATCCTGATGCGGCGCTGTATAAAATGCTTGATATGTTCTCGAGCGCGGCATTTATTATCCTT
CCGATCTTGATCGGTGTCAGTGCTGCGAAAGAGTTTGGCAGCAATCCTTACCTGGGGGCTGTACTTGGAGGAATTTTAAT
TCACCCGAGCTTGTTCAATCCTTGGGGACTTGCAACTGCATCACCGGATGTGATGGACTTTTACGGACTCAAAATCGAGA
TGCTTGGCTATCAAGGAACCGTTGTTCCAATTTTGCTTGCCGTATATGTGATGAGTAAAATCGAAAAAGGCGTAAGAAGA
ATCGTTCCAAATGCGATCGATCTATTAGTAACACCGTTTGTCACGATTATTTCAACGGGATTCATTGCTTTTATCGCGAT
CGGTCCTTTAGGAAGAATGCTTGGTACAGGGATCACGAACATTTTAACATATGTGTATGACGTTGCCGGGCCTGTTGCCG
GACTGATTTTCGGGGGAACATACTCGCTGATCGTTCTAACTGGTGTCCACCACAGTTTCCATGCGATTGAGGCCGGCCTT
ATCGCTGATATCGGCAGGAACTACCTGCTCCCGATCTGGTCTATGTCCAATGTCGCCCAAGGCGGGGCCGGACTTGCCGT
ATTCTTTTTGGCAAAGCGTGCAAAAACAAAAGAAATTGCTTTACCTGCCGCTTTCTCTTCTTTTCTCGGCATTACCGAAC
CTGTTATTTTCGGTGTCAACCTCCGTTACCGCCGGCCGTTTATCGCGGCAATGGCCGGGGGAGCGCTGGGAGCTGCATAC
GTTGTCTTTATGAATGTTGCAGCAAACGCATACGGTTTGACGGGAATTCCGATGATTGCGATTGTCGCCCCGCTTGGAAT
GGGCAACCTGATCCACTATTTAATCGGAATGGCGATTTCTGTGACTGTCGCATTTATCACAGCCTTTATCTTAGGCATTA
ATGAAGACGAGAAAAAGTGA

Upstream 100 bases:

>100_bases
GACCTAAAATTTGCTAGATGAATGGGGAAAATCTATCCTCCATTCGTTGGTGAATTTTAGGTCTTTTCTGCTTTTTTTTA
AAAAAAAAGGAGGAGTCATC

Downstream 100 bases:

>100_bases
CGGAGGGAGGGAAAACGATGAATCAAGATCAGGAGCTTCGTCAAAAGGCAATGAACCGGGTTGAAGAATACCGGGATATT
GTAAACCTTGATCCCTATCG

Product: SacP

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIHKEIAVRLIQLLGGEDNVISAAHCATRLRLVIKDEAKIDQEKVEELEGVKGAFSSSGQYQIIFGTGLVNKVYEQFAKE
LNLDQKETVQHSDAAKGKMNPLARFAKTLSNIFVPIIPAIVASGLLMGLLGMMKAFQWVDPDAALYKMLDMFSSAAFIIL
PILIGVSAAKEFGSNPYLGAVLGGILIHPSLFNPWGLATASPDVMDFYGLKIEMLGYQGTVVPILLAVYVMSKIEKGVRR
IVPNAIDLLVTPFVTIISTGFIAFIAIGPLGRMLGTGITNILTYVYDVAGPVAGLIFGGTYSLIVLTGVHHSFHAIEAGL
IADIGRNYLLPIWSMSNVAQGGAGLAVFFLAKRAKTKEIALPAAFSSFLGITEPVIFGVNLRYRRPFIAAMAGGALGAAY
VVFMNVAANAYGLTGIPMIAIVAPLGMGNLIHYLIGMAISVTVAFITAFILGINEDEKK

Sequences:

>Translated_459_residues
MIHKEIAVRLIQLLGGEDNVISAAHCATRLRLVIKDEAKIDQEKVEELEGVKGAFSSSGQYQIIFGTGLVNKVYEQFAKE
LNLDQKETVQHSDAAKGKMNPLARFAKTLSNIFVPIIPAIVASGLLMGLLGMMKAFQWVDPDAALYKMLDMFSSAAFIIL
PILIGVSAAKEFGSNPYLGAVLGGILIHPSLFNPWGLATASPDVMDFYGLKIEMLGYQGTVVPILLAVYVMSKIEKGVRR
IVPNAIDLLVTPFVTIISTGFIAFIAIGPLGRMLGTGITNILTYVYDVAGPVAGLIFGGTYSLIVLTGVHHSFHAIEAGL
IADIGRNYLLPIWSMSNVAQGGAGLAVFFLAKRAKTKEIALPAAFSSFLGITEPVIFGVNLRYRRPFIAAMAGGALGAAY
VVFMNVAANAYGLTGIPMIAIVAPLGMGNLIHYLIGMAISVTVAFITAFILGINEDEKK
>Mature_459_residues
MIHKEIAVRLIQLLGGEDNVISAAHCATRLRLVIKDEAKIDQEKVEELEGVKGAFSSSGQYQIIFGTGLVNKVYEQFAKE
LNLDQKETVQHSDAAKGKMNPLARFAKTLSNIFVPIIPAIVASGLLMGLLGMMKAFQWVDPDAALYKMLDMFSSAAFIIL
PILIGVSAAKEFGSNPYLGAVLGGILIHPSLFNPWGLATASPDVMDFYGLKIEMLGYQGTVVPILLAVYVMSKIEKGVRR
IVPNAIDLLVTPFVTIISTGFIAFIAIGPLGRMLGTGITNILTYVYDVAGPVAGLIFGGTYSLIVLTGVHHSFHAIEAGL
IADIGRNYLLPIWSMSNVAQGGAGLAVFFLAKRAKTKEIALPAAFSSFLGITEPVIFGVNLRYRRPFIAAMAGGALGAAY
VVFMNVAANAYGLTGIPMIAIVAPLGMGNLIHYLIGMAISVTVAFITAFILGINEDEKK

Specific function: Acts as both a kinase and a phosphatase on sacT [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=426, Percent_Identity=34.2723004694836, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1790159, Length=413, Percent_Identity=31.9612590799031, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788769, Length=443, Percent_Identity=29.3453724604966, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI48994906, Length=461, Percent_Identity=27.9826464208243, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49041; Mature: 49041

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHKEIAVRLIQLLGGEDNVISAAHCATRLRLVIKDEAKIDQEKVEELEGVKGAFSSSGQ
CCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHEECCCHHCCHHHHHHHHCCCHHCCCCCC
YQIIFGTGLVNKVYEQFAKELNLDQKETVQHSDAAKGKMNPLARFAKTLSNIFVPIIPAI
EEEEEECHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMMKAFQWVDPDAALYKMLDMFSSAAFIILPILIGVSAAKEFGSNPYLGA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHH
VLGGILIHPSLFNPWGLATASPDVMDFYGLKIEMLGYQGTVVPILLAVYVMSKIEKGVRR
HHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
IVPNAIDLLVTPFVTIISTGFIAFIAIGPLGRMLGTGITNILTYVYDVAGPVAGLIFGGT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
YSLIVLTGVHHSFHAIEAGLIADIGRNYLLPIWSMSNVAQGGAGLAVFFLAKRAKTKEIA
EEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCHHHHHHHHHHCCCHHHC
LPAAFSSFLGITEPVIFGVNLRYRRPFIAAMAGGALGAAYVVFMNVAANAYGLTGIPMIA
CHHHHHHHHCCCCHHEECCCEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHH
IVAPLGMGNLIHYLIGMAISVTVAFITAFILGINEDEKK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIHKEIAVRLIQLLGGEDNVISAAHCATRLRLVIKDEAKIDQEKVEELEGVKGAFSSSGQ
CCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHEECCCHHCCHHHHHHHHCCCHHCCCCCC
YQIIFGTGLVNKVYEQFAKELNLDQKETVQHSDAAKGKMNPLARFAKTLSNIFVPIIPAI
EEEEEECHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMMKAFQWVDPDAALYKMLDMFSSAAFIILPILIGVSAAKEFGSNPYLGA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHH
VLGGILIHPSLFNPWGLATASPDVMDFYGLKIEMLGYQGTVVPILLAVYVMSKIEKGVRR
HHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
IVPNAIDLLVTPFVTIISTGFIAFIAIGPLGRMLGTGITNILTYVYDVAGPVAGLIFGGT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
YSLIVLTGVHHSFHAIEAGLIADIGRNYLLPIWSMSNVAQGGAGLAVFFLAKRAKTKEIA
EEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCHHHHHHHHHHCCCHHHC
LPAAFSSFLGITEPVIFGVNLRYRRPFIAAMAGGALGAAYVVFMNVAANAYGLTGIPMIA
CHHHHHHHHCCCCHHEECCCEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHH
IVAPLGMGNLIHYLIGMAISVTVAFITAFILGINEDEKK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3122206; 7934828; 9384377; 10568751 [H]