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The map label for this gene is moaA

Identifier: 52787593

GI number: 52787593

Start: 3740073

End: 3741098

Strand: Reverse

Name: moaA

Synonym: BLi03915

Alternate gene names: 52787593

Gene position: 3741098-3740073 (Counterclockwise)

Preceding gene: 52787594

Following gene: 52787592

Centisome position: 88.6

GC content: 49.22

Gene sequence:

>1026_bases
GTGAACACCAACAGTGAACAAGTTTTGGATCAGAAAAGCCGGCCGCTTCGGGATTTAAGAATTTCTGTCACCGACCGCTG
CAACTTAAGATGTACGTATTGCATGCCTGCAGAAATCTTTGGGCAGGATTATCCGTTTTTGCCAAAAGGCGAACTGTTGT
CCTTTGAAGAGCTTGAACGATTGGCCAAGCTGTTTGTTCATCAGTTCGGGGTAGAAAAAATCCGTCTCACCGGCGGAGAG
CCGCTGATGAGAAAAGATATGCCGGAATTAGTCGGGAAGCTTGCAGGGATCAAAGGGATTCGCGATATTGCGATGACAAC
GAACGGCGTTTTGCTGCCGGTTTACGCTGACAAGCTGAAAAAAGCGGGGCTGAAACGTGTAACGGTCAGCCTGGATTCCC
TGGATGATGAGCGTTTCAAATCGATCAACGGAAGAGGCGTTTCCGTCAGCAAGGTGCTGGCCGGAATTGAAGCGGCGAAA
AAGGCCGGACTCGGCGTCAAGATCAACATGGTCGTCCAGAAAGGGGTCAATGAGAAGGATATTTTGCCGATGGCCCGCTA
TTTCAAAGAAAAAGGCCATATTTTGCGGTTTATTGAGTTTATGGATGTCGGGAACACAAATGAATGGAACCGTCAGTCAG
TGGTGGCCAAAGCGGATATCATCCGGATCATCAATGAAGATATGCCGATTGAACCGATCGACCCGAACTACGAAGGAGAA
GTCGCAAAACGATACCGATACCTGGACGGCTCAGGAGAAATCGGTTTTATCTCTTCTGTATCCGATGCTTTTTGCACCAC
ATGCAACAGAGCAAGACTATCGGCGAAAGGCGAGCTTTTCACATGTCTTTTCGCATCAAGCGGGTTTGATTTAAGAACCT
TGATCAGAAGCGGGCAGACCGATCAGGAGCTGGCGGAGGCGATCGGCACCGTCTGGCGGAATCGCGATGACCAATATTCT
CTCGACCGCGCTCTTGCAAAAGCGGTTCCGCGGAAAAAGGTTGAAATGTCCTATATTGGAGGATAA

Upstream 100 bases:

>100_bases
GGAGCTGAATATCATGACGATTGGTTTTGCGAGAAACCGGTCATTCAATGTATACACGCATCACGAGCGAATTCAGTTTT
CATAAAAGGAGGCAACAGGC

Downstream 100 bases:

>100_bases
TTGACTGCTCCTGAGAAGGCCGTCCATAGAGTAGATGGGCGGTTTTTTTCATGCTGAGGTCTGCTTGTTTTGCATATTGA
CAGGTTTGATACGGGGGAAA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNTNSEQVLDQKSRPLRDLRISVTDRCNLRCTYCMPAEIFGQDYPFLPKGELLSFEELERLAKLFVHQFGVEKIRLTGGE
PLMRKDMPELVGKLAGIKGIRDIAMTTNGVLLPVYADKLKKAGLKRVTVSLDSLDDERFKSINGRGVSVSKVLAGIEAAK
KAGLGVKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNEWNRQSVVAKADIIRIINEDMPIEPIDPNYEGE
VAKRYRYLDGSGEIGFISSVSDAFCTTCNRARLSAKGELFTCLFASSGFDLRTLIRSGQTDQELAEAIGTVWRNRDDQYS
LDRALAKAVPRKKVEMSYIGG

Sequences:

>Translated_341_residues
MNTNSEQVLDQKSRPLRDLRISVTDRCNLRCTYCMPAEIFGQDYPFLPKGELLSFEELERLAKLFVHQFGVEKIRLTGGE
PLMRKDMPELVGKLAGIKGIRDIAMTTNGVLLPVYADKLKKAGLKRVTVSLDSLDDERFKSINGRGVSVSKVLAGIEAAK
KAGLGVKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNEWNRQSVVAKADIIRIINEDMPIEPIDPNYEGE
VAKRYRYLDGSGEIGFISSVSDAFCTTCNRARLSAKGELFTCLFASSGFDLRTLIRSGQTDQELAEAIGTVWRNRDDQYS
LDRALAKAVPRKKVEMSYIGG
>Mature_341_residues
MNTNSEQVLDQKSRPLRDLRISVTDRCNLRCTYCMPAEIFGQDYPFLPKGELLSFEELERLAKLFVHQFGVEKIRLTGGE
PLMRKDMPELVGKLAGIKGIRDIAMTTNGVLLPVYADKLKKAGLKRVTVSLDSLDDERFKSINGRGVSVSKVLAGIEAAK
KAGLGVKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNEWNRQSVVAKADIIRIINEDMPIEPIDPNYEGE
VAKRYRYLDGSGEIGFISSVSDAFCTTCNRARLSAKGELFTCLFASSGFDLRTLIRSGQTDQELAEAIGTVWRNRDDQYS
LDRALAKAVPRKKVEMSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=303, Percent_Identity=40.2640264026403, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI22027536, Length=303, Percent_Identity=40.2640264026403, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1786999, Length=338, Percent_Identity=33.4319526627219, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17561210, Length=327, Percent_Identity=40.6727828746177, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32566331, Length=326, Percent_Identity=38.6503067484663, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI32566329, Length=327, Percent_Identity=39.1437308868502, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI28574490, Length=335, Percent_Identity=37.910447761194, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI28574488, Length=324, Percent_Identity=37.962962962963, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_BACLD (Q65DY5)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080993.2
- RefSeq:   YP_093422.1
- HSSP:   P65388
- ProteinModelPortal:   Q65DY5
- SMR:   Q65DY5
- STRING:   Q65DY5
- EnsemblBacteria:   EBBACT00000056702
- EnsemblBacteria:   EBBACT00000061332
- GeneID:   3028567
- GeneID:   3101215
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03915
- KEGG:   bli:BL04009
- NMPDR:   fig|279010.5.peg.4000
- eggNOG:   COG2896
- GeneTree:   EBGT00050000002384
- HOGENOM:   HBG529885
- ProtClustDB:   PRK00164
- BioCyc:   BLIC279010-1:BLI03915-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 38171; Mature: 38171

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 20-20 BINDING 33-33 BINDING 75-75 BINDING 79-79 BINDING 106-106 BINDING 130-130 BINDING 167-167 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNSEQVLDQKSRPLRDLRISVTDRCNLRCTYCMPAEIFGQDYPFLPKGELLSFEELER
CCCCHHHHHHHHCCCHHHEEEEEECCCCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHHH
LAKLFVHQFGVEKIRLTGGEPLMRKDMPELVGKLAGIKGIRDIAMTTNGVLLPVYADKLK
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEHHHHHHH
KAGLKRVTVSLDSLDDERFKSINGRGVSVSKVLAGIEAAKKAGLGVKINMVVQKGVNEKD
HCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
ILPMARYFKEKGHILRFIEFMDVGNTNEWNRQSVVAKADIIRIINEDMPIEPIDPNYEGE
HHHHHHHHHHCCHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
VAKRYRYLDGSGEIGFISSVSDAFCTTCNRARLSAKGELFTCLFASSGFDLRTLIRSGQT
HHHHHEECCCCCCEEEHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCHHHHHHHCCCC
DQELAEAIGTVWRNRDDQYSLDRALAKAVPRKKVEMSYIGG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCEEECCC
>Mature Secondary Structure
MNTNSEQVLDQKSRPLRDLRISVTDRCNLRCTYCMPAEIFGQDYPFLPKGELLSFEELER
CCCCHHHHHHHHCCCHHHEEEEEECCCCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHHH
LAKLFVHQFGVEKIRLTGGEPLMRKDMPELVGKLAGIKGIRDIAMTTNGVLLPVYADKLK
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEHHHHHHH
KAGLKRVTVSLDSLDDERFKSINGRGVSVSKVLAGIEAAKKAGLGVKINMVVQKGVNEKD
HCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
ILPMARYFKEKGHILRFIEFMDVGNTNEWNRQSVVAKADIIRIINEDMPIEPIDPNYEGE
HHHHHHHHHHCCHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
VAKRYRYLDGSGEIGFISSVSDAFCTTCNRARLSAKGELFTCLFASSGFDLRTLIRSGQT
HHHHHEECCCCCCEEEHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCHHHHHHHCCCC
DQELAEAIGTVWRNRDDQYSLDRALAKAVPRKKVEMSYIGG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA