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The map label for this gene is dhbE [H]

Identifier: 52787578

GI number: 52787578

Start: 3726263

End: 3727885

Strand: Reverse

Name: dhbE [H]

Synonym: BLi03900

Alternate gene names: 52787578

Gene position: 3727885-3726263 (Counterclockwise)

Preceding gene: 52787579

Following gene: 52787577

Centisome position: 88.28

GC content: 52.25

Gene sequence:

>1623_bases
ATGCTGAAAGGATGCACGACATGGCCGAAGGAGTTTGCGGATAAATATCGGAAGGAGGGATGCTGGCGAGGCGAAACTTT
TGGAAAAATGCTGAGGGAGCGGGCCGAAAAATACGGAAGCCGCATTGCCCTTACATACGGAGATATCCATTTAAGCTATC
GTGAGTTAGACAGGAGAGCCGATTCTTTGGCTGGCGGTCTTTTAAAGCTGGGGATCAAAAAAAATGATCGCGTCGTTGTC
CAGCTGCCGAATATCATCGAGTTTTTTGAAGTTTGCTTTGCGCTCTTCCGGATCGGAGCTCTGCCTGTTTTTGCGCTTCC
ATCGCATCGGCATAGCGAGATCACGTATTTTTGCGAGTTTTCTGAAGCTGCTGCCTACATTATTCCCGATCGTCACGCAA
AGTTTGACTACCGCAAGCTGGCGAGACAGGTAAAGGAGAAGCTGCAAGGGCTCCGTCATGTCATTGTCGCCGGGGAACCG
GAGGAGTTTGAAGCGCTGGCAAGCCTTTATGATCAGCCGGCACCTCCGCAAAACGTCTATCCAGAAGATGTCGCCTTTCT
TCAGTTGTCGGGGGGAAGCACAGGTCTGTCCAAGCTGATCCCGAGGACGCATGATGATTATATATACAGCTTGAGGGTCA
GCGCAGAGATTTGCAGGCTCGATCACAACAGCGTATATCTTGCGGCTCTTCCGATCGCTCACAATTATCCGCTCAGCTCG
CCGGGAGTGCTCGGTACGCTGTACGCGGGAGGCCGAGTCGTCCTTGCATCAGGAGGCAGCCCGGATGAAACCTTCCCTCT
GATCGATAAGGAGAGGGTAACGATCACGGGACTCGTACCGCCGCTTGCGTTAATCTGGCTTGATGCTGCTTCGGCGCGCC
GGGACGACTTGTCAAGCCTCCAGGTCTTTCAAGTCGGCGGAGCGAAATTCAGCGCCGAAGCTGCCAGACGCGTTTCATCG
GTGTTTGGCTGCAAACTGCAGCAGGTTTATGGCATGGCAGAAGGTCTGGTCAACTATACCAGACTTGATGATCCTGAAGA
CACGATCGTTCACACACAAGGCAGGCCAATGTCTCCGTTTGACGAGATTAAAATTGTGGACGAAAAAGGACGCGAACTTG
GACCCGGACAGACAGGAGAGCTGCTGACAAGAGGTCCATATACGATTCGCGGCTATTACAAAGCTGAGGAGCACAATGCC
AAAGCGTTCACTGAAGACGGGTTTTACCGTACGGGAGACTTGGTTAAAGTCAATTCATCAGGCTATTTGATTGTTGAAGG
ACGAGCTAAAGACCAGATCAACAGAGGCGGTGAGAAGGTTGCCGCGGAAGAAGTGGAAAACCATCTGCTCGCACATCCGG
GGGTACTCGATGCGGCGGTTGTCTCGATGCCGGATCAATTTCTCGGCGAAAGGGCGTGTGCATATATTATCCCTCGCGGC
GCTGCACCGGCTCAGGCTGAGTTGCAATCGTTTTTGAGAGAAAGGGGCCTTGCAGCCTACAAAATACCCGACCGGGTAGA
AATGGTTGAAGATTTCCCGCAGACAGGCGTAGGAAAAGTCAGCAAAAAAGAGCTGCGTGCGGCGATTGCTGAAAAAATGT
CTGCAGCTGAGAATGTCAGATGA

Upstream 100 bases:

>100_bases
GCCGGGTCGAAGCCTGAAGACGAACTTGCCGAAACGTCTGCAAAGTTTCGGACAATGCTTCTTGCGATGGGAATGAACAG
TGAGTAACGGAGGGATAGAG

Downstream 100 bases:

>100_bases
TAAAAAATGAGAGGATGTGATTTGATGGCCATTCCTGCCATCTTACCGTACCAAATGCCAGAAGCATCAAATATGCCGAA
AAACAAGGTGTCATGGACTC

Product: DhbE

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLKGCTTWPKEFADKYRKEGCWRGETFGKMLRERAEKYGSRIALTYGDIHLSYRELDRRADSLAGGLLKLGIKKNDRVVV
QLPNIIEFFEVCFALFRIGALPVFALPSHRHSEITYFCEFSEAAAYIIPDRHAKFDYRKLARQVKEKLQGLRHVIVAGEP
EEFEALASLYDQPAPPQNVYPEDVAFLQLSGGSTGLSKLIPRTHDDYIYSLRVSAEICRLDHNSVYLAALPIAHNYPLSS
PGVLGTLYAGGRVVLASGGSPDETFPLIDKERVTITGLVPPLALIWLDAASARRDDLSSLQVFQVGGAKFSAEAARRVSS
VFGCKLQQVYGMAEGLVNYTRLDDPEDTIVHTQGRPMSPFDEIKIVDEKGRELGPGQTGELLTRGPYTIRGYYKAEEHNA
KAFTEDGFYRTGDLVKVNSSGYLIVEGRAKDQINRGGEKVAAEEVENHLLAHPGVLDAAVVSMPDQFLGERACAYIIPRG
AAPAQAELQSFLRERGLAAYKIPDRVEMVEDFPQTGVGKVSKKELRAAIAEKMSAAENVR

Sequences:

>Translated_540_residues
MLKGCTTWPKEFADKYRKEGCWRGETFGKMLRERAEKYGSRIALTYGDIHLSYRELDRRADSLAGGLLKLGIKKNDRVVV
QLPNIIEFFEVCFALFRIGALPVFALPSHRHSEITYFCEFSEAAAYIIPDRHAKFDYRKLARQVKEKLQGLRHVIVAGEP
EEFEALASLYDQPAPPQNVYPEDVAFLQLSGGSTGLSKLIPRTHDDYIYSLRVSAEICRLDHNSVYLAALPIAHNYPLSS
PGVLGTLYAGGRVVLASGGSPDETFPLIDKERVTITGLVPPLALIWLDAASARRDDLSSLQVFQVGGAKFSAEAARRVSS
VFGCKLQQVYGMAEGLVNYTRLDDPEDTIVHTQGRPMSPFDEIKIVDEKGRELGPGQTGELLTRGPYTIRGYYKAEEHNA
KAFTEDGFYRTGDLVKVNSSGYLIVEGRAKDQINRGGEKVAAEEVENHLLAHPGVLDAAVVSMPDQFLGERACAYIIPRG
AAPAQAELQSFLRERGLAAYKIPDRVEMVEDFPQTGVGKVSKKELRAAIAEKMSAAENVR
>Mature_540_residues
MLKGCTTWPKEFADKYRKEGCWRGETFGKMLRERAEKYGSRIALTYGDIHLSYRELDRRADSLAGGLLKLGIKKNDRVVV
QLPNIIEFFEVCFALFRIGALPVFALPSHRHSEITYFCEFSEAAAYIIPDRHAKFDYRKLARQVKEKLQGLRHVIVAGEP
EEFEALASLYDQPAPPQNVYPEDVAFLQLSGGSTGLSKLIPRTHDDYIYSLRVSAEICRLDHNSVYLAALPIAHNYPLSS
PGVLGTLYAGGRVVLASGGSPDETFPLIDKERVTITGLVPPLALIWLDAASARRDDLSSLQVFQVGGAKFSAEAARRVSS
VFGCKLQQVYGMAEGLVNYTRLDDPEDTIVHTQGRPMSPFDEIKIVDEKGRELGPGQTGELLTRGPYTIRGYYKAEEHNA
KAFTEDGFYRTGDLVKVNSSGYLIVEGRAKDQINRGGEKVAAEEVENHLLAHPGVLDAAVVSMPDQFLGERACAYIIPRG
AAPAQAELQSFLRERGLAAYKIPDRVEMVEDFPQTGVGKVSKKELRAAIAEKMSAAENVR

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=25.6317689530686, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=26.9870609981516, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=26.9870609981516, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI122937307, Length=512, Percent_Identity=25.5859375, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI38505220, Length=515, Percent_Identity=23.8834951456311, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI42544132, Length=530, Percent_Identity=23.2075471698113, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI115511026, Length=539, Percent_Identity=25.7884972170686, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI28416953, Length=539, Percent_Identity=25.6029684601113, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=24.609375, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=24.609375, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI58082049, Length=509, Percent_Identity=24.3614931237721, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI8923896, Length=563, Percent_Identity=23.8010657193606, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=47.7611940298507, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI145693145, Length=536, Percent_Identity=30.2238805970149, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=27.1375464684015, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI221142682, Length=524, Percent_Identity=25.763358778626, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790505, Length=562, Percent_Identity=25.0889679715303, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786529, Length=522, Percent_Identity=23.7547892720307, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789201, Length=361, Percent_Identity=24.0997229916898, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788595, Length=510, Percent_Identity=24.1176470588235, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786801, Length=500, Percent_Identity=22.6, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538037, Length=514, Percent_Identity=29.5719844357977, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17557194, Length=508, Percent_Identity=29.1338582677165, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17560308, Length=553, Percent_Identity=27.4864376130199, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71994703, Length=568, Percent_Identity=24.8239436619718, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=568, Percent_Identity=24.8239436619718, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=568, Percent_Identity=24.8239436619718, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=512, Percent_Identity=25.1953125, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71996755, Length=515, Percent_Identity=26.0194174757282, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=24.4047619047619, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=23.4086242299795, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=378, Percent_Identity=27.2486772486773, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=536, Percent_Identity=23.3208955223881, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=387, Percent_Identity=25.5813953488372, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=573, Percent_Identity=23.9092495636998, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=573, Percent_Identity=23.9092495636998, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=357, Percent_Identity=25.4901960784314, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=535, Percent_Identity=24.8598130841122, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=536, Percent_Identity=22.7611940298507, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24581924, Length=548, Percent_Identity=27.007299270073, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI18859661, Length=485, Percent_Identity=26.1855670103093, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=24.2884250474383, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922652, Length=533, Percent_Identity=22.8893058161351, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI62472339, Length=536, Percent_Identity=26.3059701492537, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24667955, Length=535, Percent_Identity=26.1682242990654, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356441, Length=375, Percent_Identity=25.6, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24656500, Length=367, Percent_Identity=26.158038147139, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648260, Length=365, Percent_Identity=23.5616438356164, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648253, Length=540, Percent_Identity=22.7777777777778, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355225, Length=552, Percent_Identity=23.3695652173913, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24667959, Length=377, Percent_Identity=26.2599469496021, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356947, Length=378, Percent_Identity=23.2804232804233, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648255, Length=540, Percent_Identity=22.962962962963, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21358303, Length=354, Percent_Identity=22.8813559322034, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=272, Percent_Identity=27.5735294117647, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=404, Percent_Identity=24.7524752475248, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653035, Length=297, Percent_Identity=24.5791245791246, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573631, Length=544, Percent_Identity=20.5882352941176, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130353, Length=544, Percent_Identity=20.5882352941176, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161076582, Length=262, Percent_Identity=24.0458015267176, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.-

Molecular weight: Translated: 59790; Mature: 59790

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGCTTWPKEFADKYRKEGCWRGETFGKMLRERAEKYGSRIALTYGDIHLSYRELDRRA
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCEEEHHHHHHHHH
DSLAGGLLKLGIKKNDRVVVQLPNIIEFFEVCFALFRIGALPVFALPSHRHSEITYFCEF
HHHHCCHHEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEE
SEAAAYIIPDRHAKFDYRKLARQVKEKLQGLRHVIVAGEPEEFEALASLYDQPAPPQNVY
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCCCC
PEDVAFLQLSGGSTGLSKLIPRTHDDYIYSLRVSAEICRLDHNSVYLAALPIAHNYPLSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHEECCCCCEEEEEEEEECCCCCCC
PGVLGTLYAGGRVVLASGGSPDETFPLIDKERVTITGLVPPLALIWLDAASARRDDLSSL
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCE
QVFQVGGAKFSAEAARRVSSVFGCKLQQVYGMAEGLVNYTRLDDPEDTIVHTQGRPMSPF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCH
DEIKIVDEKGRELGPGQTGELLTRGPYTIRGYYKAEEHNAKAFTEDGFYRTGDLVKVNSS
HHEEEECCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCEECCCCCEECCCEEEECCC
GYLIVEGRAKDQINRGGEKVAAEEVENHLLAHPGVLDAAVVSMPDQFLGERACAYIIPRG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCEEEEEECC
AAPAQAELQSFLRERGLAAYKIPDRVEMVEDFPQTGVGKVSKKELRAAIAEKMSAAENVR
CCCHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKGCTTWPKEFADKYRKEGCWRGETFGKMLRERAEKYGSRIALTYGDIHLSYRELDRRA
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCEEEHHHHHHHHH
DSLAGGLLKLGIKKNDRVVVQLPNIIEFFEVCFALFRIGALPVFALPSHRHSEITYFCEF
HHHHCCHHEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEE
SEAAAYIIPDRHAKFDYRKLARQVKEKLQGLRHVIVAGEPEEFEALASLYDQPAPPQNVY
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCCCC
PEDVAFLQLSGGSTGLSKLIPRTHDDYIYSLRVSAEICRLDHNSVYLAALPIAHNYPLSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHEECCCCCEEEEEEEEECCCCCCC
PGVLGTLYAGGRVVLASGGSPDETFPLIDKERVTITGLVPPLALIWLDAASARRDDLSSL
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCE
QVFQVGGAKFSAEAARRVSSVFGCKLQQVYGMAEGLVNYTRLDDPEDTIVHTQGRPMSPF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCH
DEIKIVDEKGRELGPGQTGELLTRGPYTIRGYYKAEEHNAKAFTEDGFYRTGDLVKVNSS
HHEEEECCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCEECCCCCEECCCEEEECCC
GYLIVEGRAKDQINRGGEKVAAEEVENHLLAHPGVLDAAVVSMPDQFLGERACAYIIPRG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCEEEEEECC
AAPAQAELQSFLRERGLAAYKIPDRVEMVEDFPQTGVGKVSKKELRAAIAEKMSAAENVR
CCCHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]