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The map label for this gene is eno [H]

Identifier: 52787346

GI number: 52787346

Start: 3486341

End: 3487633

Strand: Reverse

Name: eno [H]

Synonym: BLi03661

Alternate gene names: 52787346

Gene position: 3487633-3486341 (Counterclockwise)

Preceding gene: 52787347

Following gene: 52787345

Centisome position: 82.59

GC content: 47.56

Gene sequence:

>1293_bases
ATGCCATACATTGTTGATGTTTATGCACGTGAAGTATTAGACTCCCGCGGTAACCCGACGGTTGAAGTTGAAGTATACAC
TGAATCAGGAGCTTTTGGACGCGCGCTGGTTCCAAGCGGAGCTTCCACTGGCGAATACGAAGCTGTTGAGCTTCGCGACG
GCGACAAAGACCGCTACCTTGGAAAAGGCGTTTTAACAGCCGTTAACAACGTGAACGAAATCATCGCACCCGAGCTTATC
GGCTTTGATGTGACTGAGCAAGTATCAATCGACAAATTGCTGATCGAACTTGACGGAACTGAAAATAAAGGCAAGCTCGG
CGCCAACGCCATCCTTGGTGTATCAATGGCCGTTGCCCGCGCAGCTGCGGATTTCTTGCAGATTCCTCTATACCAATACC
TTGGAGGATTCAACTCTAAAACGCTTCCTGTACCGATGATGAACATCGTAAACGGCGGAGAGCATGCGGACAACAACGTT
GACATTCAAGAATTCATGATCATGCCTGTCGGTGCGGAAAACTTCCGCGAAGCACTTCGCATGGGAGCACAAATTTTCCA
CAGCCTGAAATCAGTCTTGAAAGAAAAAGGCTTGAACACAGCTGTAGGTGATGAAGGCGGATTCGCTCCAAACCTTGGAT
CTAACGAAGAAGCGCTTCAAACAATCGTTGAAGCGATCGAAAAAGCAGGATTCAAACCTGGCGAAGAAGTGAAATTGGCA
ATGGATGCTGCATCTTCTGAGTTCTACAACAAAGAAGACGGCAAATACCATCTTGCAGGCGAAGGCGTTGTAAAAACGTC
AGCTGAAATGGTTGACTGGTACGAGGAGCTGACTTCTAAGTACCCAATCATCTCAATCGAAGACGGCCTTGACGAAAACG
ACTGGGAAGGCCACAAACTTCTGACTGAGCGTCTTGGCTCAAAAGTTCAGCTTGTCGGTGACGACCTTTTCGTAACAAAC
ACGAAAAAGCTTGCTGAAGGAATCAAAAACGGCGTCGGCAACTCTATCCTGATCAAAGTAAACCAAATCGGTACATTGAC
TGAAACTTTCGATGCGATCGAAATGGCGAAACGCGCAGGCTACACTGCCGTTATCTCTCACCGCTCCGGTGAAACTGAAG
ACAGCACAATCGCTGACATCGCTGTGGCAACAAACGCAGGACAAATCAAAACAGGTGCTCCGTCTCGTACGGACCGTGTT
GCGAAATACAACCAGCTTCTTCGCATCGAAGATCAATTGGCTGAAACTGCGCAATACCACGGTATTCAATCTTTCTACAA
CTTGAATAAGTAA

Upstream 100 bases:

>100_bases
CTGGCGCCAACTCTTTTAGACTTGCTGGGTGTTGAAAAACCGAAAGAAATGACAGGATCGTCATTAATTCAAAAATAAGC
ATTAAAGGAGAGAAACAAAC

Downstream 100 bases:

>100_bases
GCATAAAAAAGAGGTTGGCTGCAGATGCGGCCAACCTCTTTTAAATGTGAAAGCTGAATTTCCTTTACCCTGACAAAAGC
TGTCAGGGTTTTTGATTATA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPYIVDVYAREVLDSRGNPTVEVEVYTESGAFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPELI
GFDVTEQVSIDKLLIELDGTENKGKLGANAILGVSMAVARAAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNV
DIQEFMIMPVGAENFREALRMGAQIFHSLKSVLKEKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
MDAASSEFYNKEDGKYHLAGEGVVKTSAEMVDWYEELTSKYPIISIEDGLDENDWEGHKLLTERLGSKVQLVGDDLFVTN
TKKLAEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRV
AKYNQLLRIEDQLAETAQYHGIQSFYNLNK

Sequences:

>Translated_430_residues
MPYIVDVYAREVLDSRGNPTVEVEVYTESGAFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPELI
GFDVTEQVSIDKLLIELDGTENKGKLGANAILGVSMAVARAAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNV
DIQEFMIMPVGAENFREALRMGAQIFHSLKSVLKEKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
MDAASSEFYNKEDGKYHLAGEGVVKTSAEMVDWYEELTSKYPIISIEDGLDENDWEGHKLLTERLGSKVQLVGDDLFVTN
TKKLAEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRV
AKYNQLLRIEDQLAETAQYHGIQSFYNLNK
>Mature_429_residues
PYIVDVYAREVLDSRGNPTVEVEVYTESGAFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPELIG
FDVTEQVSIDKLLIELDGTENKGKLGANAILGVSMAVARAAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNVD
IQEFMIMPVGAENFREALRMGAQIFHSLKSVLKEKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLAM
DAASSEFYNKEDGKYHLAGEGVVKTSAEMVDWYEELTSKYPIISIEDGLDENDWEGHKLLTERLGSKVQLVGDDLFVTNT
KKLAEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVA
KYNQLLRIEDQLAETAQYHGIQSFYNLNK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=436, Percent_Identity=52.5229357798165, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=51.8348623853211, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897477, Length=440, Percent_Identity=50.2272727272727, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=440, Percent_Identity=50.2272727272727, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=438, Percent_Identity=45.4337899543379, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=25.4237288135593, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=25.4237288135593, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=25.4237288135593, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=65.0234741784037, Blast_Score=537, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71995829, Length=440, Percent_Identity=51.5909090909091, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17536383, Length=440, Percent_Identity=51.5909090909091, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32563855, Length=136, Percent_Identity=64.7058823529412, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=442, Percent_Identity=50.4524886877828, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=442, Percent_Identity=49.7737556561086, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=442, Percent_Identity=49.7737556561086, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=442, Percent_Identity=49.7737556561086, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=442, Percent_Identity=50.4524886877828, Blast_Score=378, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=51.1574074074074, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=51.1574074074074, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=51.1574074074074, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=51.1574074074074, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=51.1574074074074, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=51.1574074074074, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46639; Mature: 46508

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYIVDVYAREVLDSRGNPTVEVEVYTESGAFGRALVPSGASTGEYEAVELRDGDKDRYL
CCEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHHH
GKGVLTAVNNVNEIIAPELIGFDVTEQVSIDKLLIELDGTENKGKLGANAILGVSMAVAR
HHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNVDIQEFMIMPVGAENFREALR
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAQIFHSLKSVLKEKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDAASSEFYNKEDGKYHLAGEGVVKTSAEMVDWYEELTSKYPIISIEDGLDENDWEGHKL
EHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHH
LTERLGSKVQLVGDDLFVTNTKKLAEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDQLAETAQYH
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GIQSFYNLNK
CHHHHHCCCC
>Mature Secondary Structure 
PYIVDVYAREVLDSRGNPTVEVEVYTESGAFGRALVPSGASTGEYEAVELRDGDKDRYL
CEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHHH
GKGVLTAVNNVNEIIAPELIGFDVTEQVSIDKLLIELDGTENKGKLGANAILGVSMAVAR
HHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNVDIQEFMIMPVGAENFREALR
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAQIFHSLKSVLKEKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDAASSEFYNKEDGKYHLAGEGVVKTSAEMVDWYEELTSKYPIISIEDGLDENDWEGHKL
EHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHH
LTERLGSKVQLVGDDLFVTNTKKLAEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDQLAETAQYH
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GIQSFYNLNK
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA