Click here to switch to the map view.

The map label for this gene is pemA [H]

Identifier: 52787189

GI number: 52787189

Start: 3337357

End: 3338310

Strand: Reverse

Name: pemA [H]

Synonym: BLi03498

Alternate gene names: 52787189

Gene position: 3338310-3337357 (Counterclockwise)

Preceding gene: 52787190

Following gene: 52787188

Centisome position: 79.06

GC content: 48.85

Gene sequence:

>954_bases
GTGGTTAATCGGGAGGCAGCTGCTATACGGCTGACGGTTTCGAAGGATGGAGACGGCGAATTTCAAACCGTTCAAGAGGC
GATCGATGCTTTGCCTGAGTACAGTCGGGAACAGAAAGTGATATTCATTAAAAAAGGAGTTTACAAAGAAGTCGTTCATA
TTCCGGCTACAAAGCCGTTTGTGAAGCTGATCGGCGAAAATCGTTACGAGACTGTGATTACGTATGACAACTATGCCGGA
AAAGAAAAGGAAGGAGGAGGGAAATACGGCACGACCGGAAGTTCAAGCGTGTTTATTTATGCGGATCATGTTGAAGCCGA
AAATTTGACGTTTGAAAACTCGTTCGACCGCACGAAAGTCGACACGACCGATACGCAAGCGGTGGCGGTTTATGCGAAAG
GAAACAGGATGACGTTCAAATATGTCAGGTTTATAGGCAGACAAGATACACTGTTTGTCAATGACGGTACACAATATTTT
TATCAATGCTATATAGAAGGCGATGTCGATTTCATTTTCGGAGGAGCAAGAGCTGTGTTTGAAGAGTGCCAGATCCATAG
CGCTGACCGCGGGTCCGCTACAAACAACGGTTATGTGACAGCTGCGAGCACGCATATCGCAAAGCCGTTCGGTTTATTGA
TAACAAACTGCAGAGTGACAAGCGATGCCGCAGATGGAACGGTATACCTCGGGCGCCCATGGCATCCGGGAGGAGACCCC
GACGCCATCGCCAGTGTGCTATATCACCGCTGCGATTTAGGCGCACACATCAAGCCTGAAGGATGGACGGATATGTCGGG
CTTTTCTGCGGCCGATGCCAGATTGTACGAATACGGGAATACGGGTCCGGGTGCGATTTCGCATGAGGCCCGAAGGCAGC
TTGCCGATCATGAGGCTGAGAAATGGACAATTGAAAACGTATTGGACGGCTGGAATCCTAAAGTTGAATCATAG

Upstream 100 bases:

>100_bases
AAACGGGCCGATTGATCAAATCAAGCCGTTTTCCGGCAGGGGAAAACGGCTTTTTGTCGTAAACTTTAAGTAAGCGTTTT
CAAAGAGAGGGAGTGTTCAA

Downstream 100 bases:

>100_bases
CAAGGCAACCTGCAGAAAGACCGCTCTCTCGCTGAGATGAGCGGTCTTTTTATGCGAGCGGGTCCGATGTGCCATACGAC
CCTGGTCGTACGGCAAAATC

Product: hypothetical protein

Products: NA

Alternate protein names: PE A; Pectin methylesterase A [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MVNREAAAIRLTVSKDGDGEFQTVQEAIDALPEYSREQKVIFIKKGVYKEVVHIPATKPFVKLIGENRYETVITYDNYAG
KEKEGGGKYGTTGSSSVFIYADHVEAENLTFENSFDRTKVDTTDTQAVAVYAKGNRMTFKYVRFIGRQDTLFVNDGTQYF
YQCYIEGDVDFIFGGARAVFEECQIHSADRGSATNNGYVTAASTHIAKPFGLLITNCRVTSDAADGTVYLGRPWHPGGDP
DAIASVLYHRCDLGAHIKPEGWTDMSGFSAADARLYEYGNTGPGAISHEARRQLADHEAEKWTIENVLDGWNPKVES

Sequences:

>Translated_317_residues
MVNREAAAIRLTVSKDGDGEFQTVQEAIDALPEYSREQKVIFIKKGVYKEVVHIPATKPFVKLIGENRYETVITYDNYAG
KEKEGGGKYGTTGSSSVFIYADHVEAENLTFENSFDRTKVDTTDTQAVAVYAKGNRMTFKYVRFIGRQDTLFVNDGTQYF
YQCYIEGDVDFIFGGARAVFEECQIHSADRGSATNNGYVTAASTHIAKPFGLLITNCRVTSDAADGTVYLGRPWHPGGDP
DAIASVLYHRCDLGAHIKPEGWTDMSGFSAADARLYEYGNTGPGAISHEARRQLADHEAEKWTIENVLDGWNPKVES
>Mature_317_residues
MVNREAAAIRLTVSKDGDGEFQTVQEAIDALPEYSREQKVIFIKKGVYKEVVHIPATKPFVKLIGENRYETVITYDNYAG
KEKEGGGKYGTTGSSSVFIYADHVEAENLTFENSFDRTKVDTTDTQAVAVYAKGNRMTFKYVRFIGRQDTLFVNDGTQYF
YQCYIEGDVDFIFGGARAVFEECQIHSADRGSATNNGYVTAASTHIAKPFGLLITNCRVTSDAADGTVYLGRPWHPGGDP
DAIASVLYHRCDLGAHIKPEGWTDMSGFSAADARLYEYGNTGPGAISHEARRQLADHEAEKWTIENVLDGWNPKVES

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 35107; Mature: 35107

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00800 PECTINESTERASE_1 ; PS00503 PECTINESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNREAAAIRLTVSKDGDGEFQTVQEAIDALPEYSREQKVIFIKKGVYKEVVHIPATKPF
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHEECCCCCHH
VKLIGENRYETVITYDNYAGKEKEGGGKYGTTGSSSVFIYADHVEAENLTFENSFDRTKV
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCC
DTTDTQAVAVYAKGNRMTFKYVRFIGRQDTLFVNDGTQYFYQCYIEGDVDFIFGGARAVF
CCCCCEEEEEEECCCEEEEHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEEECCHHHHH
EECQIHSADRGSATNNGYVTAASTHIAKPFGLLITNCRVTSDAADGTVYLGRPWHPGGDP
HHHHCCCCCCCCCCCCCEEEEECCHHCCCCEEEEEEEEEECCCCCCEEEECCCCCCCCCH
DAIASVLYHRCDLGAHIKPEGWTDMSGFSAADARLYEYGNTGPGAISHEARRQLADHEAE
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHH
KWTIENVLDGWNPKVES
HEEHHHHHCCCCCCCCC
>Mature Secondary Structure
MVNREAAAIRLTVSKDGDGEFQTVQEAIDALPEYSREQKVIFIKKGVYKEVVHIPATKPF
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHEECCCCCHH
VKLIGENRYETVITYDNYAGKEKEGGGKYGTTGSSSVFIYADHVEAENLTFENSFDRTKV
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCC
DTTDTQAVAVYAKGNRMTFKYVRFIGRQDTLFVNDGTQYFYQCYIEGDVDFIFGGARAVF
CCCCCEEEEEEECCCEEEEHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEEECCHHHHH
EECQIHSADRGSATNNGYVTAASTHIAKPFGLLITNCRVTSDAADGTVYLGRPWHPGGDP
HHHHCCCCCCCCCCCCCEEEEECCHHCCCCEEEEEEEEEECCCCCCEEEECCCCCCCCCH
DAIASVLYHRCDLGAHIKPEGWTDMSGFSAADARLYEYGNTGPGAISHEARRQLADHEAE
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHH
KWTIENVLDGWNPKVES
HEEHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370537 [H]